Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannamei

Ammonia, nitrite and sulfide are aquatic systemic toxicants that threaten the health of aquatic animals. They directly affect aquatic animal survival and aquaculture productivity, and cause damage to aquatic animal tissues. This experiment investigated the effects of differences in concentrations an...

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Main Authors: Lulu Han, Peiyu Yan, Mengqiang Wang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Comparative Immunology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950311625000254
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author Lulu Han
Peiyu Yan
Mengqiang Wang
author_facet Lulu Han
Peiyu Yan
Mengqiang Wang
author_sort Lulu Han
collection DOAJ
description Ammonia, nitrite and sulfide are aquatic systemic toxicants that threaten the health of aquatic animals. They directly affect aquatic animal survival and aquaculture productivity, and cause damage to aquatic animal tissues. This experiment investigated the effects of differences in concentrations and types of toxicants in aquatic systems on the survival rate of shrimp and the degree of damage to various tissues, which will provide a reference to the maintenance of the water quality conditions in shrimp aquaculture process. All the three chemicals affected shrimp survival compared to the control, and there was no significant difference between the effects of different concentrations of ammonia (10 mg/L, 20 mg/L and 30 mg/L ammonia-N) on survival at the end of stress, while both nitrite (20 mg/L, 40 mg/L and 60 mg/L nitrite-N) and sulfide (2 mg/L, 3 mg/L and 4 mg/L sulfide) gradually decreased the survival rate at the end of stress with increasing stress concentration. Ammonia and sulfide gradually increased the damage to the hepatopancreas with increasing stress concentrations, and all the three concentrations of nitrite caused severe damage to the hepatopancreas. The separation of the epithelial cells of the midgut from the basement membrane was more pronounced with increasing stress concentrations of all the three chemicals. Dissociation of muscle tissue was more severe at higher concentrations of ammonia and sulfide, and severe dissociation of muscle occurred at all the three concentrations of nitrite. All the three chemicals showed progressively more damage to gill with increasing stress concentrations. In summary, survival and tissue damage to Litopenaeus vannamei progressively increased with increasing concentrations of ammonia, nitrite, and sulfide, with nitrite overall being more damaging to survival and tissue than ammonia and sulfide.
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spelling doaj-art-5580e63484cb42409d0811eb7738d34c2025-08-20T02:31:12ZengElsevierComparative Immunology Reports2950-31162025-06-01820021910.1016/j.cirep.2025.200219Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannameiLulu Han0Peiyu Yan1Mengqiang Wang2MOE Key Laboratory of Marine Genetics and Breeding, Shandong Key Laboratory of Marine Seed Industry (preparatory), and Qingdao Institute of Maritime Silk Road (Qingdao Institute of Blue Seed Industry), Ocean University of China, Qingdao 266003, PR China; Hainan Key Laboratory of Tropical Aquatic Germplasm, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, PR ChinaMOE Key Laboratory of Marine Genetics and Breeding, Shandong Key Laboratory of Marine Seed Industry (preparatory), and Qingdao Institute of Maritime Silk Road (Qingdao Institute of Blue Seed Industry), Ocean University of China, Qingdao 266003, PR China; Hainan Key Laboratory of Tropical Aquatic Germplasm, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, PR China; Co-Corresponding author at: MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, PR China.MOE Key Laboratory of Marine Genetics and Breeding, Shandong Key Laboratory of Marine Seed Industry (preparatory), and Qingdao Institute of Maritime Silk Road (Qingdao Institute of Blue Seed Industry), Ocean University of China, Qingdao 266003, PR China; Hainan Key Laboratory of Tropical Aquatic Germplasm, Sanya Oceanographic Institution, Ocean University of China, Sanya 572024, PR China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, PR China; Corresponding author at: MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, PR China.Ammonia, nitrite and sulfide are aquatic systemic toxicants that threaten the health of aquatic animals. They directly affect aquatic animal survival and aquaculture productivity, and cause damage to aquatic animal tissues. This experiment investigated the effects of differences in concentrations and types of toxicants in aquatic systems on the survival rate of shrimp and the degree of damage to various tissues, which will provide a reference to the maintenance of the water quality conditions in shrimp aquaculture process. All the three chemicals affected shrimp survival compared to the control, and there was no significant difference between the effects of different concentrations of ammonia (10 mg/L, 20 mg/L and 30 mg/L ammonia-N) on survival at the end of stress, while both nitrite (20 mg/L, 40 mg/L and 60 mg/L nitrite-N) and sulfide (2 mg/L, 3 mg/L and 4 mg/L sulfide) gradually decreased the survival rate at the end of stress with increasing stress concentration. Ammonia and sulfide gradually increased the damage to the hepatopancreas with increasing stress concentrations, and all the three concentrations of nitrite caused severe damage to the hepatopancreas. The separation of the epithelial cells of the midgut from the basement membrane was more pronounced with increasing stress concentrations of all the three chemicals. Dissociation of muscle tissue was more severe at higher concentrations of ammonia and sulfide, and severe dissociation of muscle occurred at all the three concentrations of nitrite. All the three chemicals showed progressively more damage to gill with increasing stress concentrations. In summary, survival and tissue damage to Litopenaeus vannamei progressively increased with increasing concentrations of ammonia, nitrite, and sulfide, with nitrite overall being more damaging to survival and tissue than ammonia and sulfide.http://www.sciencedirect.com/science/article/pii/S2950311625000254Litopenaeus vannameiAmmonia stressNitrite stressSulfide stressHistological analysis
spellingShingle Lulu Han
Peiyu Yan
Mengqiang Wang
Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannamei
Comparative Immunology Reports
Litopenaeus vannamei
Ammonia stress
Nitrite stress
Sulfide stress
Histological analysis
title Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannamei
title_full Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannamei
title_fullStr Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannamei
title_full_unstemmed Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannamei
title_short Comparative analysis on survival and tissue damage of different environmental stress factors in Pacific white shrimp Litopenaeus vannamei
title_sort comparative analysis on survival and tissue damage of different environmental stress factors in pacific white shrimp litopenaeus vannamei
topic Litopenaeus vannamei
Ammonia stress
Nitrite stress
Sulfide stress
Histological analysis
url http://www.sciencedirect.com/science/article/pii/S2950311625000254
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AT peiyuyan comparativeanalysisonsurvivalandtissuedamageofdifferentenvironmentalstressfactorsinpacificwhiteshrimplitopenaeusvannamei
AT mengqiangwang comparativeanalysisonsurvivalandtissuedamageofdifferentenvironmentalstressfactorsinpacificwhiteshrimplitopenaeusvannamei