Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig
One of the inflammatory responses of the eye to local application of platelet-activating factor (PAF) is oedema of the conjunctiva, caused by extravasation of plasma. Aim of the study was to investigate if fluorescein would leak from the blood into the tears together with plasma protein after applic...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1997-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629359791532 |
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| _version_ | 1832547430905675776 |
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| author | J. L. Van Delft F. Meijer J. A. Van Best N. J. Van Haeringen |
| author_facet | J. L. Van Delft F. Meijer J. A. Van Best N. J. Van Haeringen |
| author_sort | J. L. Van Delft |
| collection | DOAJ |
| description | One of the inflammatory responses of the eye to local application of platelet-activating factor (PAF) is oedema of the conjunctiva, caused by extravasation of plasma. Aim of the study was to investigate if fluorescein would leak from the blood into the tears together with plasma protein after application of PAF to the eye. Fluorescein was given intraperitoneally 30 min prior to application of 25 μl of 0.1% solution of PAF. Thirty min after PAF the tear film was collected by washing the surface of the eye with 25 μl of phosphate buffered saline (PBS). Fluorescein in eye washings and in plasma was measured by fluorophotometry and albumin by immunodiffusion. Both fluorescein and albumin appeared in a related fashion in tears, being absent in washings of placebo-treated control eyes. Extravasation of fluorescein can be used as a measure for plasma leakage in the conjunctiva with the advantage over the Evans Blue method that the former is a non-invasive method. |
| format | Article |
| id | doaj-art-5574a902449b4e1d822c2daabb9de2d4 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1997-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5574a902449b4e1d822c2daabb9de2d42025-02-03T06:44:45ZengWileyMediators of Inflammation0962-93511466-18611997-01-0165-638138310.1080/09629359791532Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pigJ. L. Van Delft0F. Meijer1J. A. Van Best2N. J. Van Haeringen3Department of Ophthalmology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The NetherlandsThe Netherlands Ophthalmic Research Institute, Amsterdam, The NetherlandsDepartment of Ophthalmology, Leiden University Medical Center, Albinusdreef 2, Leiden 2333 ZA, The NetherlandsThe Netherlands Ophthalmic Research Institute, Amsterdam, The NetherlandsOne of the inflammatory responses of the eye to local application of platelet-activating factor (PAF) is oedema of the conjunctiva, caused by extravasation of plasma. Aim of the study was to investigate if fluorescein would leak from the blood into the tears together with plasma protein after application of PAF to the eye. Fluorescein was given intraperitoneally 30 min prior to application of 25 μl of 0.1% solution of PAF. Thirty min after PAF the tear film was collected by washing the surface of the eye with 25 μl of phosphate buffered saline (PBS). Fluorescein in eye washings and in plasma was measured by fluorophotometry and albumin by immunodiffusion. Both fluorescein and albumin appeared in a related fashion in tears, being absent in washings of placebo-treated control eyes. Extravasation of fluorescein can be used as a measure for plasma leakage in the conjunctiva with the advantage over the Evans Blue method that the former is a non-invasive method.http://dx.doi.org/10.1080/09629359791532 |
| spellingShingle | J. L. Van Delft F. Meijer J. A. Van Best N. J. Van Haeringen Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig Mediators of Inflammation |
| title | Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig |
| title_full | Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig |
| title_fullStr | Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig |
| title_full_unstemmed | Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig |
| title_short | Permeability of blood-tear barrier to fluorescein and albumin after application of platelet-activating factor to the eye of the guinea pig |
| title_sort | permeability of blood tear barrier to fluorescein and albumin after application of platelet activating factor to the eye of the guinea pig |
| url | http://dx.doi.org/10.1080/09629359791532 |
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