Synthesis and Bioconjugation of Gold Nanoparticles as Potential Molecular Probes for Light-Based Imaging Techniques
We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles make them suitable as contrast agents for optical imaging...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2007-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/2007/29817 |
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| Summary: | We have synthesized and characterized gold nanoparticles (spheres and rods) with optical extinction bands within
the “optical imaging window.” The intense plasmon resonant driven absorption and scattering peaks of these nanoparticles
make them suitable as contrast agents for optical imaging techniques. Further, we have conjugated these gold nanoparticles
to a mouse monoclonal antibody specific to HER2 overexpressing SKBR3 breast carcinoma cells. The bioconjugation
protocol uses noncovalent modes of binding based on a combination of electrostatic and hydrophobic interactions of
the antibody and the gold surface. We discuss various aspects of the synthesis and bioconjugation protocols and the
characterization results of the functionalized nanoparticles. Some proposed applications of these potential molecular
probes in the field of biomedical imaging are also discussed. |
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| ISSN: | 1687-4188 1687-4196 |