Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone
Diabetes affects over 38 million individuals in the U.S. and is associated with a heightened risk of fractures despite normal or elevated bone mineral density (BMD). This increased fracture susceptibility may be linked to the accumulation of advanced glycation end products (AGEs), which are theorize...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | Bone Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235218722500018X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850103421969891328 |
|---|---|
| author | Katelynn R. Gallagher Olivia N. White Andrew A. Tomaschke Dyann M. Segvich Joseph M. Wallace |
| author_facet | Katelynn R. Gallagher Olivia N. White Andrew A. Tomaschke Dyann M. Segvich Joseph M. Wallace |
| author_sort | Katelynn R. Gallagher |
| collection | DOAJ |
| description | Diabetes affects over 38 million individuals in the U.S. and is associated with a heightened risk of fractures despite normal or elevated bone mineral density (BMD). This increased fracture susceptibility may be linked to the accumulation of advanced glycation end products (AGEs), which are theorized to compromise bone quality by stiffening the collagen network, leading to tissue embrittlement. In this study, the mechanical effects of AGE accumulation in human cortical bone were evaluated in vitro. Bone beams, derived from a human femur, were incubated in a ribose solution to induce AGE accumulation, while control beams were incubated in a control solution. Dynamic Mechanical Analysis (DMA) and three-point bending tests were conducted to assess the mechanical properties of the bone beams. Fluorescent AGE analysis was performed to quantify and compare AGE levels between the groups. The study found no significant differences in mechanical properties between the control and ribose-treated groups, despite a significant elevation in normalized AGE content in the ribose group. These results suggest that AGE accumulation may have a weaker impact on the mechanical properties of human bone than previously hypothesized. However, this study emphasizes the need for further research to explore the relationship between AGE accumulation and bone quality. Understanding this relationship is crucial for developing strategies to reduce fracture risk in populations with high AGE levels, such as diabetic and elderly individuals. |
| format | Article |
| id | doaj-art-555e9fe24d7f4d8a8dd3f0dfabdc7c04 |
| institution | DOAJ |
| issn | 2352-1872 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Bone Reports |
| spelling | doaj-art-555e9fe24d7f4d8a8dd3f0dfabdc7c042025-08-20T02:39:33ZengElsevierBone Reports2352-18722025-06-012510184110.1016/j.bonr.2025.101841Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical boneKatelynn R. Gallagher0Olivia N. White1Andrew A. Tomaschke2Dyann M. Segvich3Joseph M. Wallace4Weldon School of Biomedical Engineering, Department of Purdue University, 723 W Michigan St (SL220), Indianapolis, IN, USA 46202Weldon School of Biomedical Engineering, Department of Purdue University, 723 W Michigan St (SL220), Indianapolis, IN, USA 46202Weldon School of Biomedical Engineering, Department of Purdue University, 723 W Michigan St (SL220), Indianapolis, IN, USA 46202Weldon School of Biomedical Engineering, Department of Purdue University, 723 W Michigan St (SL220), Indianapolis, IN, USA 46202Corresponding author at: Weldon School of Biomedical Engineering, Purdue University, Indianapolis, IN, USA.; Weldon School of Biomedical Engineering, Department of Purdue University, 723 W Michigan St (SL220), Indianapolis, IN, USA 46202Diabetes affects over 38 million individuals in the U.S. and is associated with a heightened risk of fractures despite normal or elevated bone mineral density (BMD). This increased fracture susceptibility may be linked to the accumulation of advanced glycation end products (AGEs), which are theorized to compromise bone quality by stiffening the collagen network, leading to tissue embrittlement. In this study, the mechanical effects of AGE accumulation in human cortical bone were evaluated in vitro. Bone beams, derived from a human femur, were incubated in a ribose solution to induce AGE accumulation, while control beams were incubated in a control solution. Dynamic Mechanical Analysis (DMA) and three-point bending tests were conducted to assess the mechanical properties of the bone beams. Fluorescent AGE analysis was performed to quantify and compare AGE levels between the groups. The study found no significant differences in mechanical properties between the control and ribose-treated groups, despite a significant elevation in normalized AGE content in the ribose group. These results suggest that AGE accumulation may have a weaker impact on the mechanical properties of human bone than previously hypothesized. However, this study emphasizes the need for further research to explore the relationship between AGE accumulation and bone quality. Understanding this relationship is crucial for developing strategies to reduce fracture risk in populations with high AGE levels, such as diabetic and elderly individuals.http://www.sciencedirect.com/science/article/pii/S235218722500018XDiabetesMicrocomputed tomographyDynamic mechanical analysisRibose/ribosylationFluorescence |
| spellingShingle | Katelynn R. Gallagher Olivia N. White Andrew A. Tomaschke Dyann M. Segvich Joseph M. Wallace Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone Bone Reports Diabetes Microcomputed tomography Dynamic mechanical analysis Ribose/ribosylation Fluorescence |
| title | Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone |
| title_full | Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone |
| title_fullStr | Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone |
| title_full_unstemmed | Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone |
| title_short | Ribosylation-induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone |
| title_sort | ribosylation induced increase in advanced glycation end products has limited impacts on mechanical properties in human cortical bone |
| topic | Diabetes Microcomputed tomography Dynamic mechanical analysis Ribose/ribosylation Fluorescence |
| url | http://www.sciencedirect.com/science/article/pii/S235218722500018X |
| work_keys_str_mv | AT katelynnrgallagher ribosylationinducedincreaseinadvancedglycationendproductshaslimitedimpactsonmechanicalpropertiesinhumancorticalbone AT olivianwhite ribosylationinducedincreaseinadvancedglycationendproductshaslimitedimpactsonmechanicalpropertiesinhumancorticalbone AT andrewatomaschke ribosylationinducedincreaseinadvancedglycationendproductshaslimitedimpactsonmechanicalpropertiesinhumancorticalbone AT dyannmsegvich ribosylationinducedincreaseinadvancedglycationendproductshaslimitedimpactsonmechanicalpropertiesinhumancorticalbone AT josephmwallace ribosylationinducedincreaseinadvancedglycationendproductshaslimitedimpactsonmechanicalpropertiesinhumancorticalbone |