Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam
Introduction: Data about the prevalence of the A2142C, A2142G, and A2143G mutations in 23S rRNA gene is still limited. The aim of this study was to determine the prevalence of these mutations in 23S rRNA gene of H. pylori vietnamese strains. Methodology: One hundred and sixty-nine patients with...
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The Journal of Infection in Developing Countries
2018-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10000 |
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| author | Van Huy Tran Thi Minh Thi Ha Phan Tuong Quynh Le Viet Nhan Nguyen Trung Nam Phan Bianca Paglietti |
| author_facet | Van Huy Tran Thi Minh Thi Ha Phan Tuong Quynh Le Viet Nhan Nguyen Trung Nam Phan Bianca Paglietti |
| author_sort | Van Huy Tran |
| collection | DOAJ |
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Introduction: Data about the prevalence of the A2142C, A2142G, and A2143G mutations in 23S rRNA gene is still limited. The aim of this study was to determine the prevalence of these mutations in 23S rRNA gene of H. pylori vietnamese strains.
Methodology: One hundred and sixty-nine patients with H. pylori-positive chronic gastritis were examined. H. pylori was detected by rapid urease test and Polymerase chain reaction (PCR). Total DNA was extracted from gastric biopsy specimens. A2142C, A2142G, and A2143G mutations were detected by DNA sequencing and PCR-restriction fragment length polymorphism (PCR-RFLP).
Results: A2143G mutation was detected in 36.1% of samples, A2142G mutation in 3.6%, while A2142C mutation was not found in any case. The mixture of wild-type and mutation strains was found in 50% of specimens with A2142G, in 23% of specimens with A2143G mutation. There was no association of 23S rRNA gene point mutations with gender or age. However, an association between the heterogeneity of mutation and age was evidenced, with mean age of the group of pure A2143G higher than the group of wild-type/A2143G mixture, and rate of the wild-type/A2143G mixture higher in patients under 40 years of age.
Conclusion: A2143G mutation was prominent, while A2142C mutation was not found in the 23S rRNA gene. PCR-RFLP has revealed a reliable assay allowing a rapid and cost-effective detection of clarithromycin-resistant strains. This is useful in countries as Vietnam with high prevalence of clarithromycin-resistance before choosing optimal therapy for H. pylori eradication.
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| format | Article |
| id | doaj-art-5541a5d95c6347cabf938fbe976bf99d |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-07-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-5541a5d95c6347cabf938fbe976bf99d2025-08-20T03:52:41ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-07-01120710.3855/jidc.10000Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in VietnamVan Huy Tran0Thi Minh Thi Ha1Phan Tuong Quynh Le2Viet Nhan Nguyen3Trung Nam Phan4Bianca Paglietti5Hue University, Hue, VietnamHue University, Hue, VietnamHue University, Hue, VietnamHue University, Hue, VietnamHue University, Hue, VietnamUniversity of Sassari, Sassari, Italy Introduction: Data about the prevalence of the A2142C, A2142G, and A2143G mutations in 23S rRNA gene is still limited. The aim of this study was to determine the prevalence of these mutations in 23S rRNA gene of H. pylori vietnamese strains. Methodology: One hundred and sixty-nine patients with H. pylori-positive chronic gastritis were examined. H. pylori was detected by rapid urease test and Polymerase chain reaction (PCR). Total DNA was extracted from gastric biopsy specimens. A2142C, A2142G, and A2143G mutations were detected by DNA sequencing and PCR-restriction fragment length polymorphism (PCR-RFLP). Results: A2143G mutation was detected in 36.1% of samples, A2142G mutation in 3.6%, while A2142C mutation was not found in any case. The mixture of wild-type and mutation strains was found in 50% of specimens with A2142G, in 23% of specimens with A2143G mutation. There was no association of 23S rRNA gene point mutations with gender or age. However, an association between the heterogeneity of mutation and age was evidenced, with mean age of the group of pure A2143G higher than the group of wild-type/A2143G mixture, and rate of the wild-type/A2143G mixture higher in patients under 40 years of age. Conclusion: A2143G mutation was prominent, while A2142C mutation was not found in the 23S rRNA gene. PCR-RFLP has revealed a reliable assay allowing a rapid and cost-effective detection of clarithromycin-resistant strains. This is useful in countries as Vietnam with high prevalence of clarithromycin-resistance before choosing optimal therapy for H. pylori eradication. https://jidc.org/index.php/journal/article/view/10000Clarithromycin resistanceA2142GA2143GA2142C23S rRNA geneHelicobacter pylori |
| spellingShingle | Van Huy Tran Thi Minh Thi Ha Phan Tuong Quynh Le Viet Nhan Nguyen Trung Nam Phan Bianca Paglietti Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam Journal of Infection in Developing Countries Clarithromycin resistance A2142G A2143G A2142C 23S rRNA gene Helicobacter pylori |
| title | Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam |
| title_full | Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam |
| title_fullStr | Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam |
| title_full_unstemmed | Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam |
| title_short | Helicobacter pylori 23S rRNA gene mutations associated with clarithromycin resistance in chronic gastritis in Vietnam |
| title_sort | helicobacter pylori 23s rrna gene mutations associated with clarithromycin resistance in chronic gastritis in vietnam |
| topic | Clarithromycin resistance A2142G A2143G A2142C 23S rRNA gene Helicobacter pylori |
| url | https://jidc.org/index.php/journal/article/view/10000 |
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