Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD).
In humans, mutations in electron transfer flavoprotein (ETF) or electron transfer flavoprotein dehydrogenase (ETFDH) lead to MADD/glutaric aciduria type II, an autosomal recessively inherited disorder characterized by a broad spectrum of devastating neurological, systemic and metabolic symptoms. We...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-12-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0008329&type=printable |
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| author | Yuanquan Song Mary A Selak Corey T Watson Christopher Coutts Paul C Scherer Jessica A Panzer Sarah Gibbs Marion O Scott Gregory Willer Ronald G Gregg Declan W Ali Michael J Bennett Rita J Balice-Gordon |
| author_facet | Yuanquan Song Mary A Selak Corey T Watson Christopher Coutts Paul C Scherer Jessica A Panzer Sarah Gibbs Marion O Scott Gregory Willer Ronald G Gregg Declan W Ali Michael J Bennett Rita J Balice-Gordon |
| author_sort | Yuanquan Song |
| collection | DOAJ |
| description | In humans, mutations in electron transfer flavoprotein (ETF) or electron transfer flavoprotein dehydrogenase (ETFDH) lead to MADD/glutaric aciduria type II, an autosomal recessively inherited disorder characterized by a broad spectrum of devastating neurological, systemic and metabolic symptoms. We show that a zebrafish mutant in ETFDH, xavier, and fibroblast cells from MADD patients demonstrate similar mitochondrial and metabolic abnormalities, including reduced oxidative phosphorylation, increased aerobic glycolysis, and upregulation of the PPARG-ERK pathway. This metabolic dysfunction is associated with aberrant neural proliferation in xav, in addition to other neural phenotypes and paralysis. Strikingly, a PPARG antagonist attenuates aberrant neural proliferation and alleviates paralysis in xav, while PPARG agonists increase neural proliferation in wild type embryos. These results show that mitochondrial dysfunction, leading to an increase in aerobic glycolysis, affects neurogenesis through the PPARG-ERK pathway, a potential target for therapeutic intervention. |
| format | Article |
| id | doaj-art-55359c39e9ca49cea285c1631d908dbe |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2009-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-55359c39e9ca49cea285c1631d908dbe2025-08-20T03:19:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-12-01412e832910.1371/journal.pone.0008329Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD).Yuanquan SongMary A SelakCorey T WatsonChristopher CouttsPaul C SchererJessica A PanzerSarah GibbsMarion O ScottGregory WillerRonald G GreggDeclan W AliMichael J BennettRita J Balice-GordonIn humans, mutations in electron transfer flavoprotein (ETF) or electron transfer flavoprotein dehydrogenase (ETFDH) lead to MADD/glutaric aciduria type II, an autosomal recessively inherited disorder characterized by a broad spectrum of devastating neurological, systemic and metabolic symptoms. We show that a zebrafish mutant in ETFDH, xavier, and fibroblast cells from MADD patients demonstrate similar mitochondrial and metabolic abnormalities, including reduced oxidative phosphorylation, increased aerobic glycolysis, and upregulation of the PPARG-ERK pathway. This metabolic dysfunction is associated with aberrant neural proliferation in xav, in addition to other neural phenotypes and paralysis. Strikingly, a PPARG antagonist attenuates aberrant neural proliferation and alleviates paralysis in xav, while PPARG agonists increase neural proliferation in wild type embryos. These results show that mitochondrial dysfunction, leading to an increase in aerobic glycolysis, affects neurogenesis through the PPARG-ERK pathway, a potential target for therapeutic intervention.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0008329&type=printable |
| spellingShingle | Yuanquan Song Mary A Selak Corey T Watson Christopher Coutts Paul C Scherer Jessica A Panzer Sarah Gibbs Marion O Scott Gregory Willer Ronald G Gregg Declan W Ali Michael J Bennett Rita J Balice-Gordon Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD). PLoS ONE |
| title | Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD). |
| title_full | Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD). |
| title_fullStr | Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD). |
| title_full_unstemmed | Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD). |
| title_short | Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD). |
| title_sort | mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl coa dehydrogenase deficiency madd |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0008329&type=printable |
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