Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activities
Chemical compositions and the antifungal efficacy of essential oils derived from Origanum elongatum, Mentha pulegium, Thymus vulgaris, or Corymbia citriodora were assessed against the grapevine gray mold pathogen Botrytis cinerea, isolated from Moroccan vineyards. Gas chromatography-mass spectrometr...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Firenze University Press
2025-05-01
|
| Series: | Phytopathologia Mediterranea |
| Subjects: | |
| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/15566 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850072891231567872 |
|---|---|
| author | Faical AOUJIL Loubna DRA Chaimae EL GHDAICH Sarah TOUFIQ Hiba YAHYAOUI Majida HAFIDI Aziz AZIZ Khaoula HABBADI |
| author_facet | Faical AOUJIL Loubna DRA Chaimae EL GHDAICH Sarah TOUFIQ Hiba YAHYAOUI Majida HAFIDI Aziz AZIZ Khaoula HABBADI |
| author_sort | Faical AOUJIL |
| collection | DOAJ |
| description | Chemical compositions and the antifungal efficacy of essential oils derived from Origanum elongatum, Mentha pulegium, Thymus vulgaris, or Corymbia citriodora were assessed against the grapevine gray mold pathogen Botrytis cinerea, isolated from Moroccan vineyards. Gas chromatography-mass spectrometry (GC-MS) analyses identified the major constituents of these oils as carvacrol (61.8%) from O. elongatum, pulegone (91.2%) from M. pulegium, thymol (47.8%) from T. vulgaris, and cineol (78.11%) from C. citriodora. All these essential oils had antifungal activity, inhibiting in vitro colony radial growth and conidium germination of B. cinerea. Among the essential oils, that from O. elongatum exhibited the greatest inhibition of mycelium growth, with minimum inhibitory concentrations (MICs) and effective concentrations (EC50), respectively, of 252.5 µL L-1 and 33.27 µL L-1 in direct contact, and 56.17 µL L-1 and 12.75 µL L-1 in fumigation. At 125 µL L-1, origanum essential oil completely inhibited B. cinerea conidium germination. In vivo tests with detached leaves of two grapevine cultivars and grape berries showed that essential oils from M. pulegium and O. elongatum reduced the lesion diameters by, respectively, 78% and 72% on the leaves, and by 58% and 50% on grape berries. The results indicate the potential of using these essential oils as natural and effective alternatives to chemical fungicides for control of B. cinerea, offering a promising strategy for sustainable and environmentally friendly disease management practices. |
| format | Article |
| id | doaj-art-552a8bea92d548b6aa7d8c38af3d7072 |
| institution | DOAJ |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj-art-552a8bea92d548b6aa7d8c38af3d70722025-08-20T02:46:59ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952025-05-0164110912710.36253/phyto-1556614403Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activitiesFaical AOUJIL0https://orcid.org/0009-0004-3763-9841Loubna DRA1Chaimae EL GHDAICH2https://orcid.org/0009-0005-0469-8105Sarah TOUFIQ3https://orcid.org/0009-0008-2293-9007Hiba YAHYAOUI4Majida HAFIDI5https://orcid.org/0000-0002-6207-2822Aziz AZIZ6https://orcid.org/0000-0003-1602-2506Khaoula HABBADI7https://orcid.org/0000-0002-1890-4601Phytobacteriolgy and Biological Control Laboratory, Regional Center of Agricultural Research of Meknes, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principal, Rabat 10090, MoroccoPhytobacteriolgy and Biological Control Laboratory, Regional Center of Agricultural Research of Meknes, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principal, Rabat 10090, MoroccoPhytobacteriolgy and Biological Control Laboratory, Regional Center of Agricultural Research of Meknes, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principal, Rabat 10090, MoroccoFaculty of Agricultural Science, University of Hohenheim, Stuttgart 70593, GermanyPhytobacteriolgy and Biological Control Laboratory, Regional Center of Agricultural Research of Meknes, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principal, Rabat 10090, MoroccoLaboratory of Biotechnology and Bio-Resources Valorization, Faculty of Sciences, Moulay Ismail University, Meknes 50000, MoroccoResearch Unit “Induced Resistance and Plant Bioprotection”, RIBP-USC INRAe 1488, University of Reims Champagne-Ardenne, 51100 Reims, FranceLaboratory of Biotechnology and Bio-Resources Valorization, Faculty of Sciences, Moulay Ismail University, Meknes 50000, MoroccoChemical compositions and the antifungal efficacy of essential oils derived from Origanum elongatum, Mentha pulegium, Thymus vulgaris, or Corymbia citriodora were assessed against the grapevine gray mold pathogen Botrytis cinerea, isolated from Moroccan vineyards. Gas chromatography-mass spectrometry (GC-MS) analyses identified the major constituents of these oils as carvacrol (61.8%) from O. elongatum, pulegone (91.2%) from M. pulegium, thymol (47.8%) from T. vulgaris, and cineol (78.11%) from C. citriodora. All these essential oils had antifungal activity, inhibiting in vitro colony radial growth and conidium germination of B. cinerea. Among the essential oils, that from O. elongatum exhibited the greatest inhibition of mycelium growth, with minimum inhibitory concentrations (MICs) and effective concentrations (EC50), respectively, of 252.5 µL L-1 and 33.27 µL L-1 in direct contact, and 56.17 µL L-1 and 12.75 µL L-1 in fumigation. At 125 µL L-1, origanum essential oil completely inhibited B. cinerea conidium germination. In vivo tests with detached leaves of two grapevine cultivars and grape berries showed that essential oils from M. pulegium and O. elongatum reduced the lesion diameters by, respectively, 78% and 72% on the leaves, and by 58% and 50% on grape berries. The results indicate the potential of using these essential oils as natural and effective alternatives to chemical fungicides for control of B. cinerea, offering a promising strategy for sustainable and environmentally friendly disease management practices.https://oajournals.fupress.net/index.php/pm/article/view/15566 vitis viniferaplant extractschemical compositionsustainable agriculture |
| spellingShingle | Faical AOUJIL Loubna DRA Chaimae EL GHDAICH Sarah TOUFIQ Hiba YAHYAOUI Majida HAFIDI Aziz AZIZ Khaoula HABBADI Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activities Phytopathologia Mediterranea vitis vinifera plant extracts chemical composition sustainable agriculture |
| title | Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activities |
| title_full | Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activities |
| title_fullStr | Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activities |
| title_full_unstemmed | Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activities |
| title_short | Antifungal efficacy of four plant-derived essential oils against Botrytis cinerea: chemical profiles and biological activities |
| title_sort | antifungal efficacy of four plant derived essential oils against botrytis cinerea chemical profiles and biological activities |
| topic | vitis vinifera plant extracts chemical composition sustainable agriculture |
| url | https://oajournals.fupress.net/index.php/pm/article/view/15566 |
| work_keys_str_mv | AT faicalaoujil antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities AT loubnadra antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities AT chaimaeelghdaich antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities AT sarahtoufiq antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities AT hibayahyaoui antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities AT majidahafidi antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities AT azizaziz antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities AT khaoulahabbadi antifungalefficacyoffourplantderivedessentialoilsagainstbotrytiscinereachemicalprofilesandbiologicalactivities |