Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin
Purpose. The transcription factor Krüppel-like factor 6 (KLF6) regulates various cellular functions, such as metabolism, cell proliferation, and differentiation. KLF6 plays a key role in the development and progression of multiple human cancers. Methods. Fifty primary biopsies and 10 normal nasophar...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2018/9654067 |
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| author | Saoussen Debouki-Joudi Sonia Mhirsi Nihel Ammous-Boukhris Nehla Mokni-Baizig Hayet Mhamdi Said Gritli Raja Mokdad Gargouri Mohamed Nejib Marzouki |
| author_facet | Saoussen Debouki-Joudi Sonia Mhirsi Nihel Ammous-Boukhris Nehla Mokni-Baizig Hayet Mhamdi Said Gritli Raja Mokdad Gargouri Mohamed Nejib Marzouki |
| author_sort | Saoussen Debouki-Joudi |
| collection | DOAJ |
| description | Purpose. The transcription factor Krüppel-like factor 6 (KLF6) regulates various cellular functions, such as metabolism, cell proliferation, and differentiation. KLF6 plays a key role in the development and progression of multiple human cancers. Methods. Fifty primary biopsies and 10 normal nasopharyngeal mucosae were used to analyze by RT-QPCR the expression and the copy number of wtKLF6 and the spliced variants (KLF6-SV1, KLF6-SV2, and KLF6-SV3) in Tunisian patients with nasopharyngeal carcinoma. The expression analysis of E-cadherin and cyclin D1 was conducted by RT-QPCR and Western blot, respectively. Results. The wtKLF6 was significantly downexpressed in tumors compared to normal tissues (p=0.0015), whereas KLF6-SV1 and KLF6-SV2 were overexpressed in tumors compared to wtKLF6 and KLF6-SV3 (p<0.0001). Copy number variation was reduced in tumors compared to normal tissues (p=0.0071). Interestingly, KLF6-SV1 is associated with the juvenile form (p=0.0003) which is more aggressive than the adult form of NPC. Furthermore, the oncogenic variant KLF6-SV1 was overexpressed in tumors lacking the expression of E-cadherin (p=0.0022) suggesting its role in metastasis and tumor progression. The wtKLF6 is associated negatively with cyclin D1 in tumor tissues (p=0.048). Conclusion. The wtKLF6 was downexpressed in contrast with the oncogenic variants. Overexpression of KLF6-SV1 is associated with young patients, and loss of E-cadherin suggests that this variant correlated with the aggressiveness of NPC. |
| format | Article |
| id | doaj-art-551f8e94fcac45abb362c26b005b2177 |
| institution | OA Journals |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-551f8e94fcac45abb362c26b005b21772025-08-20T02:05:07ZengWileyAnalytical Cellular Pathology2210-71772210-71852018-01-01201810.1155/2018/96540679654067Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-CadherinSaoussen Debouki-Joudi0Sonia Mhirsi1Nihel Ammous-Boukhris2Nehla Mokni-Baizig3Hayet Mhamdi4Said Gritli5Raja Mokdad Gargouri6Mohamed Nejib Marzouki7Laboratoire d'Ingénierie des Protéines et des Molécules Bioactives, Institut National des Sciences Appliquées et de Technologie (INSAT), Tunis, TunisiaLaboratoire d'Ingénierie des Protéines et des Molécules Bioactives, Institut National des Sciences Appliquées et de Technologie (INSAT), Tunis, TunisiaLaboratoire de Biotechnologie Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax, TunisiaInstitut de Carcinologie Salah Azaiz, Tunis, TunisiaInstitut de Carcinologie Salah Azaiz, Tunis, TunisiaInstitut de Carcinologie Salah Azaiz, Tunis, TunisiaLaboratoire de Biotechnologie Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax, TunisiaLaboratoire d'Ingénierie des Protéines et des Molécules Bioactives, Institut National des Sciences Appliquées et de Technologie (INSAT), Tunis, TunisiaPurpose. The transcription factor Krüppel-like factor 6 (KLF6) regulates various cellular functions, such as metabolism, cell proliferation, and differentiation. KLF6 plays a key role in the development and progression of multiple human cancers. Methods. Fifty primary biopsies and 10 normal nasopharyngeal mucosae were used to analyze by RT-QPCR the expression and the copy number of wtKLF6 and the spliced variants (KLF6-SV1, KLF6-SV2, and KLF6-SV3) in Tunisian patients with nasopharyngeal carcinoma. The expression analysis of E-cadherin and cyclin D1 was conducted by RT-QPCR and Western blot, respectively. Results. The wtKLF6 was significantly downexpressed in tumors compared to normal tissues (p=0.0015), whereas KLF6-SV1 and KLF6-SV2 were overexpressed in tumors compared to wtKLF6 and KLF6-SV3 (p<0.0001). Copy number variation was reduced in tumors compared to normal tissues (p=0.0071). Interestingly, KLF6-SV1 is associated with the juvenile form (p=0.0003) which is more aggressive than the adult form of NPC. Furthermore, the oncogenic variant KLF6-SV1 was overexpressed in tumors lacking the expression of E-cadherin (p=0.0022) suggesting its role in metastasis and tumor progression. The wtKLF6 is associated negatively with cyclin D1 in tumor tissues (p=0.048). Conclusion. The wtKLF6 was downexpressed in contrast with the oncogenic variants. Overexpression of KLF6-SV1 is associated with young patients, and loss of E-cadherin suggests that this variant correlated with the aggressiveness of NPC.http://dx.doi.org/10.1155/2018/9654067 |
| spellingShingle | Saoussen Debouki-Joudi Sonia Mhirsi Nihel Ammous-Boukhris Nehla Mokni-Baizig Hayet Mhamdi Said Gritli Raja Mokdad Gargouri Mohamed Nejib Marzouki Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin Analytical Cellular Pathology |
| title | Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin |
| title_full | Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin |
| title_fullStr | Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin |
| title_full_unstemmed | Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin |
| title_short | Overexpression of the Oncogenic Variant (KLF6-SV1) in Young NPC Patients and Correlation with Lack of E-Cadherin |
| title_sort | overexpression of the oncogenic variant klf6 sv1 in young npc patients and correlation with lack of e cadherin |
| url | http://dx.doi.org/10.1155/2018/9654067 |
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