Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis
Toll-like receptor (TLR) 10, mainly expressed on B cells, has emerged as a modulatory receptor in inflammation. Nonetheless, the clinical significance of TLR10 in rheumatoid arthritis (RA) remains unclear. In this study, we explored the expression of TLR10 in B cells and B cell subsets in RA subject...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/9372436 |
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| author | Ying Zhang Rong Cao Haijian Ying Juping Du Shuaishuai Chen Na Wang Bo Shen |
| author_facet | Ying Zhang Rong Cao Haijian Ying Juping Du Shuaishuai Chen Na Wang Bo Shen |
| author_sort | Ying Zhang |
| collection | DOAJ |
| description | Toll-like receptor (TLR) 10, mainly expressed on B cells, has emerged as a modulatory receptor in inflammation. Nonetheless, the clinical significance of TLR10 in rheumatoid arthritis (RA) remains unclear. In this study, we explored the expression of TLR10 in B cells and B cell subsets in RA subjects and healthy controls (HCs) and determined its relevance to disease activity and inflammatory biomarkers. TLR10 levels in B cells and B cell subsets (CD19+CD27+, CD19+CD27−, CD27+IgD−, CD27+IgD+, CD27−IgD+, D27−IgD−, CD19+CD5+, and CD19+CD5−) and inflammatory biomarker concentrations in peripheral blood (PB) obtained from RA subjects and HCs were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. The correlations of TLR10 expression with disease activity and inflammatory biomarkers were then analysed. Similar levels of TLR10 in all CD19+ B cells were observed in the RA subjects and HCs. Compared to that in the HCs, TLR10 was elevated significantly in the CD19+CD27−IgD− and CD19+CD5+ subsets in the RA subjects. In addition, almost all subsets expressing TLR10 were increased with disease activity. The present study reveals that enhanced TLR10 in B cell subsets is positively correlated with disease activity in RA subjects. |
| format | Article |
| id | doaj-art-550bf3ef8fb74cafa71ed4cf209bba25 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-550bf3ef8fb74cafa71ed4cf209bba252025-02-03T05:45:55ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/93724369372436Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid ArthritisYing Zhang0Rong Cao1Haijian Ying2Juping Du3Shuaishuai Chen4Na Wang5Bo Shen6School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaSchool of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaDepartment of Clinical Laboratory, Taizhou Hospital of Zhejiang Province, Taizhou Enze Medical Center (Group), Taizhou, Zhejiang, ChinaDepartment of Clinical Laboratory, Taizhou Hospital of Zhejiang Province, Taizhou Enze Medical Center (Group), Taizhou, Zhejiang, ChinaDepartment of Clinical Laboratory, Taizhou Hospital of Zhejiang Province, Taizhou Enze Medical Center (Group), Taizhou, Zhejiang, ChinaDepartment of Clinical Laboratory, Taizhou Hospital of Zhejiang Province, Taizhou Enze Medical Center (Group), Taizhou, Zhejiang, ChinaSchool of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, ChinaToll-like receptor (TLR) 10, mainly expressed on B cells, has emerged as a modulatory receptor in inflammation. Nonetheless, the clinical significance of TLR10 in rheumatoid arthritis (RA) remains unclear. In this study, we explored the expression of TLR10 in B cells and B cell subsets in RA subjects and healthy controls (HCs) and determined its relevance to disease activity and inflammatory biomarkers. TLR10 levels in B cells and B cell subsets (CD19+CD27+, CD19+CD27−, CD27+IgD−, CD27+IgD+, CD27−IgD+, D27−IgD−, CD19+CD5+, and CD19+CD5−) and inflammatory biomarker concentrations in peripheral blood (PB) obtained from RA subjects and HCs were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. The correlations of TLR10 expression with disease activity and inflammatory biomarkers were then analysed. Similar levels of TLR10 in all CD19+ B cells were observed in the RA subjects and HCs. Compared to that in the HCs, TLR10 was elevated significantly in the CD19+CD27−IgD− and CD19+CD5+ subsets in the RA subjects. In addition, almost all subsets expressing TLR10 were increased with disease activity. The present study reveals that enhanced TLR10 in B cell subsets is positively correlated with disease activity in RA subjects.http://dx.doi.org/10.1155/2018/9372436 |
| spellingShingle | Ying Zhang Rong Cao Haijian Ying Juping Du Shuaishuai Chen Na Wang Bo Shen Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis Mediators of Inflammation |
| title | Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis |
| title_full | Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis |
| title_fullStr | Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis |
| title_full_unstemmed | Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis |
| title_short | Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis |
| title_sort | increased expression of tlr10 in b cell subsets correlates with disease activity in rheumatoid arthritis |
| url | http://dx.doi.org/10.1155/2018/9372436 |
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