Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis
ABSTRACT The declining birth rates and fertility challenges in Europe have intensified global concerns over rising infertility, particularly among women. This study decisively investigates follicular fluid‐related extracellular vesicles (FF‐EVs) from infertile patients with polycystic ovary syndrome...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-03-01
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| Series: | Journal of Extracellular Biology |
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| Online Access: | https://doi.org/10.1002/jex2.70041 |
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| author | Solène Ducarre Regina Maria Chiechio Gregory Moulin Ester Butera Aurélien Dupont Christophe Penno Abdelhak El Amrani Claire Heichette Pascale Even‐Hernandez Valérie Marchi Célia Ravel |
| author_facet | Solène Ducarre Regina Maria Chiechio Gregory Moulin Ester Butera Aurélien Dupont Christophe Penno Abdelhak El Amrani Claire Heichette Pascale Even‐Hernandez Valérie Marchi Célia Ravel |
| author_sort | Solène Ducarre |
| collection | DOAJ |
| description | ABSTRACT The declining birth rates and fertility challenges in Europe have intensified global concerns over rising infertility, particularly among women. This study decisively investigates follicular fluid‐related extracellular vesicles (FF‐EVs) from infertile patients with polycystic ovary syndrome (PCOS) or diminished ovarian reserve (DOR) undergoing in vitro fertilization (IVF), comparing them to a healthy control group. We have identified significant variations in protein content and polydispersity in crude follicular fluid using UV‐Vis absorption and dynamic light scattering (DLS) techniques. Furthermore, the morphology of the extracellular vesicles (EVs) and the patterns of non‐coding RNA content, including miRNAs, reveal distinct differences in infertile patients. These findings offer critical insights into the molecular signatures associated with these conditions. This study plays a vital role in advancing reproductive healthcare by pinpointing potential targets that can enhance diagnosis and deepen our understanding of ovarian disorders. |
| format | Article |
| id | doaj-art-54fe4110f6b34e549eeffe790cc3e053 |
| institution | OA Journals |
| issn | 2768-2811 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Biology |
| spelling | doaj-art-54fe4110f6b34e549eeffe790cc3e0532025-08-20T01:50:22ZengWileyJournal of Extracellular Biology2768-28112025-03-0143n/an/a10.1002/jex2.70041Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content AnalysisSolène Ducarre0Regina Maria Chiechio1Gregory Moulin2Ester Butera3Aurélien Dupont4Christophe Penno5Abdelhak El Amrani6Claire Heichette7Pascale Even‐Hernandez8Valérie Marchi9Célia Ravel10Institut des Sciences Chimiques de Rennes ISCR UMR CNRS 6226 Université de Rennes Rennes FranceInstitut des Sciences Chimiques de Rennes ISCR UMR CNRS 6226 Université de Rennes Rennes FranceCHU Rennes Service de Biologie de la Reproduction‐CECOS Rennes FranceInstitut des Sciences Chimiques de Rennes ISCR UMR CNRS 6226 Université de Rennes Rennes FranceUMR6 UMS 3480 US_S 018, BIOSIT, Univ. Rennes, CNRS, Inserm Rennes FranceECOBIO UMR 6553 CNRS Université de Rennes Rennes FranceECOBIO UMR 6553 CNRS Université de Rennes Rennes FranceUMR6 UMS 3480 US_S 018, BIOSIT, Univ. Rennes, CNRS, Inserm Rennes FranceInstitut des Sciences Chimiques de Rennes ISCR UMR CNRS 6226 Université de Rennes Rennes FranceInstitut des Sciences Chimiques de Rennes ISCR UMR CNRS 6226 Université de Rennes Rennes FranceInserm, EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail) – UMR_S 1085 Université de Rennes Rennes FranceABSTRACT The declining birth rates and fertility challenges in Europe have intensified global concerns over rising infertility, particularly among women. This study decisively investigates follicular fluid‐related extracellular vesicles (FF‐EVs) from infertile patients with polycystic ovary syndrome (PCOS) or diminished ovarian reserve (DOR) undergoing in vitro fertilization (IVF), comparing them to a healthy control group. We have identified significant variations in protein content and polydispersity in crude follicular fluid using UV‐Vis absorption and dynamic light scattering (DLS) techniques. Furthermore, the morphology of the extracellular vesicles (EVs) and the patterns of non‐coding RNA content, including miRNAs, reveal distinct differences in infertile patients. These findings offer critical insights into the molecular signatures associated with these conditions. This study plays a vital role in advancing reproductive healthcare by pinpointing potential targets that can enhance diagnosis and deepen our understanding of ovarian disorders.https://doi.org/10.1002/jex2.70041extracellular vesicles (EVs)polycystic ovarian syndrome (PCOS)diminished ovarian reserve (DOR)follicular fluid (FF)morphologyCryo‐TEM |
| spellingShingle | Solène Ducarre Regina Maria Chiechio Gregory Moulin Ester Butera Aurélien Dupont Christophe Penno Abdelhak El Amrani Claire Heichette Pascale Even‐Hernandez Valérie Marchi Célia Ravel Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis Journal of Extracellular Biology extracellular vesicles (EVs) polycystic ovarian syndrome (PCOS) diminished ovarian reserve (DOR) follicular fluid (FF) morphology Cryo‐TEM |
| title | Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis |
| title_full | Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis |
| title_fullStr | Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis |
| title_full_unstemmed | Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis |
| title_short | Extracellular Vesicles From Follicular Fluid in Infertile Women: Size, Morphology and miRNA Content Analysis |
| title_sort | extracellular vesicles from follicular fluid in infertile women size morphology and mirna content analysis |
| topic | extracellular vesicles (EVs) polycystic ovarian syndrome (PCOS) diminished ovarian reserve (DOR) follicular fluid (FF) morphology Cryo‐TEM |
| url | https://doi.org/10.1002/jex2.70041 |
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