Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantation
Background: The global prevalence of obesity and type 2 diabetes, particularly among children, is rising, yet the long-term impacts of early-life fecal microbiota transplantation (FMT) on metabolic health remain poorly understood. Objectives: To investigate how early-life FMT from children to young,...
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Elsevier
2025-07-01
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| Series: | Molecular Metabolism |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221287782500064X |
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| author | Federica La Rosa Maria Angela Guzzardi Mercedes Pardo-Tendero Monica Barone Chiara Ruocco Gabriele Conti Daniele Panetta Daria Riabitch Silvia Bernardi Assuero Giorgetti Daniela Campani Daniel Monleon Enzo Nisoli Patrizia Brigidi Patricia Iozzo |
| author_facet | Federica La Rosa Maria Angela Guzzardi Mercedes Pardo-Tendero Monica Barone Chiara Ruocco Gabriele Conti Daniele Panetta Daria Riabitch Silvia Bernardi Assuero Giorgetti Daniela Campani Daniel Monleon Enzo Nisoli Patrizia Brigidi Patricia Iozzo |
| author_sort | Federica La Rosa |
| collection | DOAJ |
| description | Background: The global prevalence of obesity and type 2 diabetes, particularly among children, is rising, yet the long-term impacts of early-life fecal microbiota transplantation (FMT) on metabolic health remain poorly understood. Objectives: To investigate how early-life FMT from children to young, sex-matched mice influences metabolic outcomes and adipose tissue function in later, adult life. Methods: Germ-free mice were colonized with fecal microbiota from either lean children or children with obesity. The impacts on brown adipose tissue (BAT), white adipose tissue (WAT), glucose metabolism, and gut health were analyzed in male and female mice. Microbial communities and metabolite profiles were characterized using sequencing and metabolomics. Results: Male mice receiving FMT from obese donors exhibited marked BAT whitening and impaired amino acid and glucose metabolism. In contrast, female recipients developed hyperglycemia, accompanied by gut barrier dysfunction and WAT impairment. Distinct microbial and metabolite profiles were associated with these phenotypes: Collinsella and trimethylamine in females; and Paraprevotella, Collinsella, Lachnospiraceae NK4A136, Bacteroides, Coprobacillus, and multiple metabolites in males. These phenotypic effects persisted despite changes in host environment and diet. Conclusions: Early-life FMT induced long-lasting effects on the metabolic landscape, profoundly affecting adipose tissue function and systemic glucose homeostasis in adulthood. Donor dietary habits correlated with the fecal microbial profiles observed in recipient mice. These findings highlight the critical need for identifying and leveraging beneficial exposures during early development to combat obesity and diabetes. |
| format | Article |
| id | doaj-art-54fa7a4e77e94d72bf1524d0ca3c83ef |
| institution | OA Journals |
| issn | 2212-8778 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
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| series | Molecular Metabolism |
| spelling | doaj-art-54fa7a4e77e94d72bf1524d0ca3c83ef2025-08-20T02:30:47ZengElsevierMolecular Metabolism2212-87782025-07-019710215710.1016/j.molmet.2025.102157Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantationFederica La Rosa0Maria Angela Guzzardi1Mercedes Pardo-Tendero2Monica Barone3Chiara Ruocco4Gabriele Conti5Daniele Panetta6Daria Riabitch7Silvia Bernardi8Assuero Giorgetti9Daniela Campani10Daniel Monleon11Enzo Nisoli12Patrizia Brigidi13Patricia Iozzo14Institute of Clinical Physiology, National Research Council, via Moruzzi 1, 56124 Pisa, ItalyInstitute of Clinical Physiology, National Research Council, via Moruzzi 1, 56124 Pisa, Italy; Corresponding author.Department of Pathology, University of Valencia, Health Research Institute INCLIVA/CIBERFES for Frailty and Healthy Aging, Blasco Ibañez, 15, 46010, Valencia, SpainHuman Microbiomics Unit, Department of Medical and Surgical Sciences, University of Bologna, via Massarenti 9, 40138 Bologna ItalyCenter of Study and Research on Obesity, Department of Medical Biotechnology and Translational Medicine, University of Milan, via Vanvitelli 32, 20129 Milan, ItalyHuman Microbiomics Unit, Department of Medical and Surgical Sciences, University of Bologna, via Massarenti 9, 40138 Bologna ItalyInstitute of Clinical Physiology, National Research Council, via Moruzzi 1, 56124 Pisa, ItalyInstitute of Clinical Physiology, National Research Council, via Moruzzi 1, 56124 Pisa, ItalyInstitute of Clinical Physiology, National Research Council, via Moruzzi 1, 56124 Pisa, ItalyFondazione Toscana Gabriele Monasterio, via Moruzzi 1, 56124 Pisa, ItalyDepartment of Surgical, Medical, Molecular Pathology and Critical Care Medicine, Division of Pathology, Pisa University Hospital, 56124 Pisa, ItalyDepartment of Pathology, University of Valencia, Health Research Institute INCLIVA/CIBERFES for Frailty and Healthy Aging, Blasco Ibañez, 15, 46010, Valencia, SpainCenter of Study and Research on Obesity, Department of Medical Biotechnology and Translational Medicine, University of Milan, via Vanvitelli 32, 20129 Milan, ItalyHuman Microbiomics Unit, Department of Medical and Surgical Sciences, University of Bologna, via Massarenti 9, 40138 Bologna ItalyInstitute of Clinical Physiology, National Research Council, via Moruzzi 1, 56124 Pisa, Italy; Corresponding author.Background: The global prevalence of obesity and type 2 diabetes, particularly among children, is rising, yet the long-term impacts of early-life fecal microbiota transplantation (FMT) on metabolic health remain poorly understood. Objectives: To investigate how early-life FMT from children to young, sex-matched mice influences metabolic outcomes and adipose tissue function in later, adult life. Methods: Germ-free mice were colonized with fecal microbiota from either lean children or children with obesity. The impacts on brown adipose tissue (BAT), white adipose tissue (WAT), glucose metabolism, and gut health were analyzed in male and female mice. Microbial communities and metabolite profiles were characterized using sequencing and metabolomics. Results: Male mice receiving FMT from obese donors exhibited marked BAT whitening and impaired amino acid and glucose metabolism. In contrast, female recipients developed hyperglycemia, accompanied by gut barrier dysfunction and WAT impairment. Distinct microbial and metabolite profiles were associated with these phenotypes: Collinsella and trimethylamine in females; and Paraprevotella, Collinsella, Lachnospiraceae NK4A136, Bacteroides, Coprobacillus, and multiple metabolites in males. These phenotypic effects persisted despite changes in host environment and diet. Conclusions: Early-life FMT induced long-lasting effects on the metabolic landscape, profoundly affecting adipose tissue function and systemic glucose homeostasis in adulthood. Donor dietary habits correlated with the fecal microbial profiles observed in recipient mice. These findings highlight the critical need for identifying and leveraging beneficial exposures during early development to combat obesity and diabetes.http://www.sciencedirect.com/science/article/pii/S221287782500064XObesityType 2 diabetesPositron emission tomographyGut microbiotaMetabolomicsAdipose tissue |
| spellingShingle | Federica La Rosa Maria Angela Guzzardi Mercedes Pardo-Tendero Monica Barone Chiara Ruocco Gabriele Conti Daniele Panetta Daria Riabitch Silvia Bernardi Assuero Giorgetti Daniela Campani Daniel Monleon Enzo Nisoli Patrizia Brigidi Patricia Iozzo Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantation Molecular Metabolism Obesity Type 2 diabetes Positron emission tomography Gut microbiota Metabolomics Adipose tissue |
| title | Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantation |
| title_full | Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantation |
| title_fullStr | Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantation |
| title_full_unstemmed | Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantation |
| title_short | Effects of children's microbiota on adipose and intestinal development in sex-matched mice persist into adulthood following a single fecal microbiota transplantation |
| title_sort | effects of children s microbiota on adipose and intestinal development in sex matched mice persist into adulthood following a single fecal microbiota transplantation |
| topic | Obesity Type 2 diabetes Positron emission tomography Gut microbiota Metabolomics Adipose tissue |
| url | http://www.sciencedirect.com/science/article/pii/S221287782500064X |
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