Electrophysiological properties of embryonic stem cell-derived neurons.
In vitro generation of functional neurons from embryonic stem (ES) cells and induced pluripotent stem cells offers exciting opportunities for dissecting gene function, disease modelling, and therapeutic drug screening. To realize the potential of stem cells in these biomedical applications, a comple...
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| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0024169&type=printable |
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| author | Jessica R Risner-Janiczek Mark A Ungless Meng Li |
| author_facet | Jessica R Risner-Janiczek Mark A Ungless Meng Li |
| author_sort | Jessica R Risner-Janiczek |
| collection | DOAJ |
| description | In vitro generation of functional neurons from embryonic stem (ES) cells and induced pluripotent stem cells offers exciting opportunities for dissecting gene function, disease modelling, and therapeutic drug screening. To realize the potential of stem cells in these biomedical applications, a complete understanding of the cell models of interest is required. While rapid advances have been made in developing the technologies for directed induction of defined neuronal subtypes, most published works focus on the molecular characterization of the derived neural cultures. To characterize the functional properties of these neural cultures, we utilized an ES cell model that gave rise to neurons expressing the green fluorescent protein (GFP) and conducted targeted whole-cell electrophysiological recordings from ES cell-derived neurons. Current-clamp recordings revealed that most neurons could fire single overshooting action potentials; in some cases multiple action potentials could be evoked by depolarization, or occurred spontaneously. Voltage-clamp recordings revealed that neurons exhibited neuronal-like currents, including an outward current typical of a delayed rectifier potassium conductance and a fast-activating, fast-inactivating inward current, typical of a sodium conductance. Taken together, these results indicate that ES cell-derived GFP(+) neurons in culture display functional neuronal properties even at early stages of differentiation. |
| format | Article |
| id | doaj-art-54f35ea47643489d863bd6447102fa03 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-54f35ea47643489d863bd6447102fa032025-08-20T02:30:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0168e2416910.1371/journal.pone.0024169Electrophysiological properties of embryonic stem cell-derived neurons.Jessica R Risner-JaniczekMark A UnglessMeng LiIn vitro generation of functional neurons from embryonic stem (ES) cells and induced pluripotent stem cells offers exciting opportunities for dissecting gene function, disease modelling, and therapeutic drug screening. To realize the potential of stem cells in these biomedical applications, a complete understanding of the cell models of interest is required. While rapid advances have been made in developing the technologies for directed induction of defined neuronal subtypes, most published works focus on the molecular characterization of the derived neural cultures. To characterize the functional properties of these neural cultures, we utilized an ES cell model that gave rise to neurons expressing the green fluorescent protein (GFP) and conducted targeted whole-cell electrophysiological recordings from ES cell-derived neurons. Current-clamp recordings revealed that most neurons could fire single overshooting action potentials; in some cases multiple action potentials could be evoked by depolarization, or occurred spontaneously. Voltage-clamp recordings revealed that neurons exhibited neuronal-like currents, including an outward current typical of a delayed rectifier potassium conductance and a fast-activating, fast-inactivating inward current, typical of a sodium conductance. Taken together, these results indicate that ES cell-derived GFP(+) neurons in culture display functional neuronal properties even at early stages of differentiation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0024169&type=printable |
| spellingShingle | Jessica R Risner-Janiczek Mark A Ungless Meng Li Electrophysiological properties of embryonic stem cell-derived neurons. PLoS ONE |
| title | Electrophysiological properties of embryonic stem cell-derived neurons. |
| title_full | Electrophysiological properties of embryonic stem cell-derived neurons. |
| title_fullStr | Electrophysiological properties of embryonic stem cell-derived neurons. |
| title_full_unstemmed | Electrophysiological properties of embryonic stem cell-derived neurons. |
| title_short | Electrophysiological properties of embryonic stem cell-derived neurons. |
| title_sort | electrophysiological properties of embryonic stem cell derived neurons |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0024169&type=printable |
| work_keys_str_mv | AT jessicarrisnerjaniczek electrophysiologicalpropertiesofembryonicstemcellderivedneurons AT markaungless electrophysiologicalpropertiesofembryonicstemcellderivedneurons AT mengli electrophysiologicalpropertiesofembryonicstemcellderivedneurons |