Genetic markers of insulin resistance in gestational diabetes

Aim. To search for genetic markers of insulin resistance and impaired insulin secretion in pregnant women with gestational diabetes mellitus (GDM). Materials and methods. A total of 100 healthy pregnant women and 185 patients with GDM were available for examination. 80 patients developedGDM during...

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Main Authors: Tatiana Vasil'evna Sebko, Yulia Eduardovna Dobrokhotova, Tatiana Alekseevna Ivanova, Valery Vyacheslavovich Nosikov, Tatiana Alekseevna Osipova, Victor Andreevich Potapov
Format: Article
Language:English
Published: Endocrinology Research Centre 2009-12-01
Series:Сахарный диабет
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Online Access:https://www.dia-endojournals.ru/jour/article/view/5702
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author Tatiana Vasil'evna Sebko
Yulia Eduardovna Dobrokhotova
Tatiana Alekseevna Ivanova
Valery Vyacheslavovich Nosikov
Tatiana Alekseevna Osipova
Victor Andreevich Potapov
author_facet Tatiana Vasil'evna Sebko
Yulia Eduardovna Dobrokhotova
Tatiana Alekseevna Ivanova
Valery Vyacheslavovich Nosikov
Tatiana Alekseevna Osipova
Victor Andreevich Potapov
author_sort Tatiana Vasil'evna Sebko
collection DOAJ
description Aim. To search for genetic markers of insulin resistance and impaired insulin secretion in pregnant women with gestational diabetes mellitus (GDM). Materials and methods. A total of 100 healthy pregnant women and 185 patients with GDM were available for examination. 80 patients developedGDM during current pregnancy, in 105 it was diagnosed 4-19 years ago. 25 of the 105 GDM patients had a history of type 2 DM. The following parameterswere measured: beta-cell secretory activity (proinsulin, ITI, C-peptide), total cholesterol (CH), HDL and LDL CH, triglycerides, HbA1c,fasting glycemia. Molecular-genetic DNA testing using PCR included studies of KCNJ 11, TCF7L2, PPARG2, ADIPOQ, ADIPOR1, ADIPOR2gene polymorphism. These genes were chosen based on the published data associating them with disturbed insulin secretion and sensitivity in DM2patient. Results. Pregnant women with GDM and obesity showed elevated IRI and leptin levels compared with controls. This rise was accompanied bymarked insulin resistance in 75% of these patients. In 50% of the healthy women proinsulin and insulin secretion decreased. Obesity in pregnantpatients was associated with significant elevation of proinsulin, IRI, and C-peptyide levels and GDM with Lys/Lys genotype of polymorphous markerGlu23k of KCNJ11 gene, pro and ala allele of polymorphous marker A219T of ADIPOR2 gene. These associations suggest specific genetic featuresof GDM related to impaired insulin secretion and sensitivity. Conclusion. Studies of common genetic nature of GDM and DM2 permit to identify risk groups at the preclinical stage, plan prevention and treatmentof these disorders.
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spelling doaj-art-54edd9215bed43689089f31bc64aa5652025-08-20T03:48:28ZengEndocrinology Research CentreСахарный диабет2072-03512072-03782009-12-01124384110.14341/2072-0351-57025660Genetic markers of insulin resistance in gestational diabetesTatiana Vasil'evna Sebko0Yulia Eduardovna Dobrokhotova1Tatiana Alekseevna Ivanova2Valery Vyacheslavovich Nosikov3Tatiana Alekseevna Osipova4Victor Andreevich Potapov5Russian State Medical University, MoscowRussian State Medical University, MoscowRussian State Medical University, MoscowState Research Institute of Genetics, MoscowEFIS,Ltd, MoscowState Research Institute of Genetics, MoscowAim. To search for genetic markers of insulin resistance and impaired insulin secretion in pregnant women with gestational diabetes mellitus (GDM). Materials and methods. A total of 100 healthy pregnant women and 185 patients with GDM were available for examination. 80 patients developedGDM during current pregnancy, in 105 it was diagnosed 4-19 years ago. 25 of the 105 GDM patients had a history of type 2 DM. The following parameterswere measured: beta-cell secretory activity (proinsulin, ITI, C-peptide), total cholesterol (CH), HDL and LDL CH, triglycerides, HbA1c,fasting glycemia. Molecular-genetic DNA testing using PCR included studies of KCNJ 11, TCF7L2, PPARG2, ADIPOQ, ADIPOR1, ADIPOR2gene polymorphism. These genes were chosen based on the published data associating them with disturbed insulin secretion and sensitivity in DM2patient. Results. Pregnant women with GDM and obesity showed elevated IRI and leptin levels compared with controls. This rise was accompanied bymarked insulin resistance in 75% of these patients. In 50% of the healthy women proinsulin and insulin secretion decreased. Obesity in pregnantpatients was associated with significant elevation of proinsulin, IRI, and C-peptyide levels and GDM with Lys/Lys genotype of polymorphous markerGlu23k of KCNJ11 gene, pro and ala allele of polymorphous marker A219T of ADIPOR2 gene. These associations suggest specific genetic featuresof GDM related to impaired insulin secretion and sensitivity. Conclusion. Studies of common genetic nature of GDM and DM2 permit to identify risk groups at the preclinical stage, plan prevention and treatmentof these disorders.https://www.dia-endojournals.ru/jour/article/view/5702gestational diabetes mellitusgenetic markersinsulin resistanceobesity
spellingShingle Tatiana Vasil'evna Sebko
Yulia Eduardovna Dobrokhotova
Tatiana Alekseevna Ivanova
Valery Vyacheslavovich Nosikov
Tatiana Alekseevna Osipova
Victor Andreevich Potapov
Genetic markers of insulin resistance in gestational diabetes
Сахарный диабет
gestational diabetes mellitus
genetic markers
insulin resistance
obesity
title Genetic markers of insulin resistance in gestational diabetes
title_full Genetic markers of insulin resistance in gestational diabetes
title_fullStr Genetic markers of insulin resistance in gestational diabetes
title_full_unstemmed Genetic markers of insulin resistance in gestational diabetes
title_short Genetic markers of insulin resistance in gestational diabetes
title_sort genetic markers of insulin resistance in gestational diabetes
topic gestational diabetes mellitus
genetic markers
insulin resistance
obesity
url https://www.dia-endojournals.ru/jour/article/view/5702
work_keys_str_mv AT tatianavasilevnasebko geneticmarkersofinsulinresistanceingestationaldiabetes
AT yuliaeduardovnadobrokhotova geneticmarkersofinsulinresistanceingestationaldiabetes
AT tatianaalekseevnaivanova geneticmarkersofinsulinresistanceingestationaldiabetes
AT valeryvyacheslavovichnosikov geneticmarkersofinsulinresistanceingestationaldiabetes
AT tatianaalekseevnaosipova geneticmarkersofinsulinresistanceingestationaldiabetes
AT victorandreevichpotapov geneticmarkersofinsulinresistanceingestationaldiabetes