Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesis
Myosin VI is the only actin-based motor known to move toward the minus end of actin filaments. This protein is involved in many different cellular processes, such as endocytosis, autophagy, secretion, and regulation of actin organization and dynamics. Myosin VI has also been suggested to play an imp...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1535117/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850157187679125504 |
|---|---|
| author | Robert Lenartowski Robert Lenartowski Jakub Ostrowski Anna Suwińska Anna Suwińska Anna Richert Przemysław Zakrzewski Magdalena Izdebska Wioletta Arendt Kathryn G. Miller Marta Lenartowska Marta Lenartowska |
| author_facet | Robert Lenartowski Robert Lenartowski Jakub Ostrowski Anna Suwińska Anna Suwińska Anna Richert Przemysław Zakrzewski Magdalena Izdebska Wioletta Arendt Kathryn G. Miller Marta Lenartowska Marta Lenartowska |
| author_sort | Robert Lenartowski |
| collection | DOAJ |
| description | Myosin VI is the only actin-based motor known to move toward the minus end of actin filaments. This protein is involved in many different cellular processes, such as endocytosis, autophagy, secretion, and regulation of actin organization and dynamics. Myosin VI has also been suggested to play an important role in collective migration of border cells and egg chamber development during Drosophila oogenesis. Here we show for the first time that myosin VI is expressed in Drosophila germarium as well as in early ovarian follicles, especially in the developing oocyte. As oogenesis progresses, the level of myosin VI in maturing egg chambers decreases, but this protein is present both in the nascent border cell cluster, during its delamination from the epithelium, and then during the early stages of border cell migration. However, we demonstrate that myosin VI deficiency in border cells, or even complete lack of this protein in myosin VI mutant do not inhibit border cell migration. Moreover, deficiency/lack of myosin VI does not cause any serious defects in ovarian morphology, egg chamber morphogenesis, oogenesis, and egg development. Thus we conclude that myosin VI is not a key player in Drosophila oogenesis. |
| format | Article |
| id | doaj-art-54ca8de16d1441cba0974a59fcd0eaed |
| institution | OA Journals |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-54ca8de16d1441cba0974a59fcd0eaed2025-08-20T02:24:15ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-06-011310.3389/fcell.2025.15351171535117Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesisRobert Lenartowski0Robert Lenartowski1Jakub Ostrowski2Anna Suwińska3Anna Suwińska4Anna Richert5Przemysław Zakrzewski6Magdalena Izdebska7Wioletta Arendt8Kathryn G. Miller9Marta Lenartowska10Marta Lenartowska11Department of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, Torun, PolandDepartment of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Torun, PolandDepartment of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, Torun, PolandDepartment of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Torun, PolandSchool of Cellular and Molecular Medicine, Faculty of Life Sciences, University of Bristol, Bristol, United KingdomDepartment of Histology and Embryology, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Torun, PolandDepartment of Histology and Embryology, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Torun, PolandDepartment of Biology, Washington University in St. Louis, St. Louis, MO, United StatesDepartment of Cellular and Molecular Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, Torun, PolandMyosin VI is the only actin-based motor known to move toward the minus end of actin filaments. This protein is involved in many different cellular processes, such as endocytosis, autophagy, secretion, and regulation of actin organization and dynamics. Myosin VI has also been suggested to play an important role in collective migration of border cells and egg chamber development during Drosophila oogenesis. Here we show for the first time that myosin VI is expressed in Drosophila germarium as well as in early ovarian follicles, especially in the developing oocyte. As oogenesis progresses, the level of myosin VI in maturing egg chambers decreases, but this protein is present both in the nascent border cell cluster, during its delamination from the epithelium, and then during the early stages of border cell migration. However, we demonstrate that myosin VI deficiency in border cells, or even complete lack of this protein in myosin VI mutant do not inhibit border cell migration. Moreover, deficiency/lack of myosin VI does not cause any serious defects in ovarian morphology, egg chamber morphogenesis, oogenesis, and egg development. Thus we conclude that myosin VI is not a key player in Drosophila oogenesis.https://www.frontiersin.org/articles/10.3389/fcell.2025.1535117/fullbinary systemborder cell migrationcell/molecular biologyDrosophila oogenesisRNAimyosin VI mutant |
| spellingShingle | Robert Lenartowski Robert Lenartowski Jakub Ostrowski Anna Suwińska Anna Suwińska Anna Richert Przemysław Zakrzewski Magdalena Izdebska Wioletta Arendt Kathryn G. Miller Marta Lenartowska Marta Lenartowska Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesis Frontiers in Cell and Developmental Biology binary system border cell migration cell/molecular biology Drosophila oogenesis RNAi myosin VI mutant |
| title | Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesis |
| title_full | Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesis |
| title_fullStr | Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesis |
| title_full_unstemmed | Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesis |
| title_short | Myosin VI is expressed in developing ovarian follicles in Drosophila but is not essential for effective oogenesis |
| title_sort | myosin vi is expressed in developing ovarian follicles in drosophila but is not essential for effective oogenesis |
| topic | binary system border cell migration cell/molecular biology Drosophila oogenesis RNAi myosin VI mutant |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1535117/full |
| work_keys_str_mv | AT robertlenartowski myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT robertlenartowski myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT jakubostrowski myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT annasuwinska myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT annasuwinska myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT annarichert myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT przemysławzakrzewski myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT magdalenaizdebska myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT wiolettaarendt myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT kathryngmiller myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT martalenartowska myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis AT martalenartowska myosinviisexpressedindevelopingovarianfolliclesindrosophilabutisnotessentialforeffectiveoogenesis |