Effect of baicalein on the differentiation of Naïve CD4+T cells into Th1 cells and Treg cells
Objective To investigate the effect of baicalein on the differentiation of Naïve CD4+ T cells into Th1 cells and Treg cells. Methods Naïve CD4+ T cells were isolated from the spleen of male BALB/c mice aged 6-8 weeks and were divided into groups A to F. The cells in group A were inoculated into a we...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Precision Medicine
2025-02-01
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| Series: | 精准医学杂志 |
| Subjects: | |
| Online Access: | https://jpmed.qdu.edu.cn/fileup/2096-529X/PDF/1744957601340-2055959425.pdf |
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| Summary: | Objective To investigate the effect of baicalein on the differentiation of Naïve CD4+ T cells into Th1 cells and Treg cells. Methods Naïve CD4+ T cells were isolated from the spleen of male BALB/c mice aged 6-8 weeks and were divided into groups A to F. The cells in group A were inoculated into a well plate coated with anti-mouse CD3 antibody and were cultured in a medium containing anti-mouse CD28 antibody for 72 h; in addition to the treatment in group A, the cells in group B were treated with interleukin-2 (IL-2), IL-12, and anti-mouse IL-4 antibodies; in addition to the treatment in group B, the cells in group C were further treated with baicalein. The cells in group D were cultured in the same culture system as group A; in addition to the treatment in group D, the cells in group E were further treated with IL-2 and transforming growth factor-β (TGF-β); in addition to the treatment in group E, the cells in group F were further treated with baicalein. Flow cytometry was used to measure the percen-tage of Th1 cells in groups A to C and the percentage of Treg cells in groups D to F; RT-qPCR was used to measure the relative expression levels of T-box transcription factor (T-bet), interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) in groups A to C and the relative expression levels of forkhead box P3 (Foxp3) and IL-10 in groups D to F; immunofluorescent staining was used to measure the positive rate of Th1 cells in groups A to C and the positive rate of Treg cells in groups D to F. Results Flow cytometry showed that group C had a significantly lower percentage of Th1 cells than group B (F=46.91,q=7.95,P<0.05), and group F had a significantly higher percentage of Treg cells than group E (F=145.20,q=8.64,P<0.05). RT-qPCR showed that group C had significantly lower relative expression levels of T-bet, IFN-γ, and TNF-α than group B (F=15.77-862.50,q=7.05-26.34,P<0.05), and group F had significantly higher relative expression levels of Foxp3 and IL-10 than group E (F=38.25,49.24,q=4.65,7.64,P<0.05). Immunofluorescence assay showed that group C had a significantly lower positive rate of Th1 cells than group B (F=11.64,q=4.50,P<0.05), and group F had a significantly higher positive rate of Treg cells than group E (F=20.31,q=4.64,P<0.05). Conclusion Baicalein has an inhibitory effect on the differentiation of Naïve CD4+ T cells into Th1 cells and a promotional effect on the differentiation of Naïve CD4+ T cells into Treg cells. |
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| ISSN: | 2096-529X |