Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In Vitro
Tea polyphenols have been reported to decrease the rate of starch hydrolysis by inhibiting α-glucosidase. However, the effect of the tea harvesting season and the structure of catechin monomers on the inhibitory activity of α-glucosidase is not understood. In this study, the inhibitory effect and un...
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2024-12-01
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| author | Rourou Wen Xianghua Chai Pingping Wang Kegang Wu Xuejuan Duan Jiasi Chen Tong Zhang Liya Zeng |
| author_facet | Rourou Wen Xianghua Chai Pingping Wang Kegang Wu Xuejuan Duan Jiasi Chen Tong Zhang Liya Zeng |
| author_sort | Rourou Wen |
| collection | DOAJ |
| description | Tea polyphenols have been reported to decrease the rate of starch hydrolysis by inhibiting α-glucosidase. However, the effect of the tea harvesting season and the structure of catechin monomers on the inhibitory activity of α-glucosidase is not understood. In this study, the inhibitory effect and underlying mechanism of four seasons of Dancong tea against α-glucosidase were investigated by in vivo and in vitro experiments, multi-spectroscope and molecular dynamic. The Dancong tea harvested in spring and winter showed a stronger inhibitory effect on α-glucosidase due to a higher content of catechin, especially EGCG ((-)-epigallocatechin-3-gallate). The results of in vivo and in vitro experiments showed that EGCG and ECG ((-)-epicatechin-3-gallate) with a higher content of gallate and hydroxyl groups exhibited a stronger inhibitory effect on starch hydrolysis, rise of postprandial blood glucose and activities of α-glucosidase compared to EGC ((-)-epigallocatechin) and EC ((-)-epicatechin). These gallate and hydroxy groups were more effective in interacting with the amino acid residues in the active site of α-glucosidase, leading to structural changes in the enzyme. Certainly, the inhibitory effect of Dancong tea on α-glucosidase explains one of the mechanisms by which it helps alleviate diabetes; the other hypoglycaemic mechanisms of Dancong tea will be further explored. |
| format | Article |
| id | doaj-art-54a8f1c834db49f7858ad87b67af80ea |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-54a8f1c834db49f7858ad87b67af80ea2025-08-20T02:53:33ZengMDPI AGFoods2304-81582024-12-011324418310.3390/foods13244183Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In VitroRourou Wen0Xianghua Chai1Pingping Wang2Kegang Wu3Xuejuan Duan4Jiasi Chen5Tong Zhang6Liya Zeng7School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaSchool of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, ChinaTea polyphenols have been reported to decrease the rate of starch hydrolysis by inhibiting α-glucosidase. However, the effect of the tea harvesting season and the structure of catechin monomers on the inhibitory activity of α-glucosidase is not understood. In this study, the inhibitory effect and underlying mechanism of four seasons of Dancong tea against α-glucosidase were investigated by in vivo and in vitro experiments, multi-spectroscope and molecular dynamic. The Dancong tea harvested in spring and winter showed a stronger inhibitory effect on α-glucosidase due to a higher content of catechin, especially EGCG ((-)-epigallocatechin-3-gallate). The results of in vivo and in vitro experiments showed that EGCG and ECG ((-)-epicatechin-3-gallate) with a higher content of gallate and hydroxyl groups exhibited a stronger inhibitory effect on starch hydrolysis, rise of postprandial blood glucose and activities of α-glucosidase compared to EGC ((-)-epigallocatechin) and EC ((-)-epicatechin). These gallate and hydroxy groups were more effective in interacting with the amino acid residues in the active site of α-glucosidase, leading to structural changes in the enzyme. Certainly, the inhibitory effect of Dancong tea on α-glucosidase explains one of the mechanisms by which it helps alleviate diabetes; the other hypoglycaemic mechanisms of Dancong tea will be further explored.https://www.mdpi.com/2304-8158/13/24/4183Phoenix single dancong teacatechin monomersEGCGenzyme inhibition |
| spellingShingle | Rourou Wen Xianghua Chai Pingping Wang Kegang Wu Xuejuan Duan Jiasi Chen Tong Zhang Liya Zeng Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In Vitro Foods Phoenix single dancong tea catechin monomers EGCG enzyme inhibition |
| title | Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In Vitro |
| title_full | Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In Vitro |
| title_fullStr | Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In Vitro |
| title_full_unstemmed | Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In Vitro |
| title_short | Inhibitory Effect and Mechanism of Dancong Tea from Different Harvesting Season on the α-Glucosidase Inhibition In Vivo and In Vitro |
| title_sort | inhibitory effect and mechanism of dancong tea from different harvesting season on the α glucosidase inhibition in vivo and in vitro |
| topic | Phoenix single dancong tea catechin monomers EGCG enzyme inhibition |
| url | https://www.mdpi.com/2304-8158/13/24/4183 |
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