The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth
Adipose issue is a source of mesenchymal stem cells (MSC) that can be used to stimulate blood vessel growth in ischemic tissues. Various metabolicdisorders including hypeglycemia may have negative effect on therapeutic properties of these cells. Aim. To study the influence of high glucose concentra...
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| Format: | Article |
| Language: | English |
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Endocrinology Research Centre
2011-06-01
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| Series: | Сахарный диабет |
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| Online Access: | https://www.dia-endojournals.ru/jour/article/view/5631 |
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| author | Zhanna Alekseevna Akopyan Georgy Vladimirovich Sharonov Tatiana Nikolaevna Kochegura Natalya Fedorovna Il'yashenko Igor Eduardovich Belyanko Valentina Ivanovna Dimitrova Andrey Evgen'evich Zotikov Natalya Igorevna Kalinina Elena Viktorovna Parfenova |
| author_facet | Zhanna Alekseevna Akopyan Georgy Vladimirovich Sharonov Tatiana Nikolaevna Kochegura Natalya Fedorovna Il'yashenko Igor Eduardovich Belyanko Valentina Ivanovna Dimitrova Andrey Evgen'evich Zotikov Natalya Igorevna Kalinina Elena Viktorovna Parfenova |
| author_sort | Zhanna Alekseevna Akopyan |
| collection | DOAJ |
| description | Adipose issue is a source of mesenchymal stem cells (MSC) that can be used to stimulate blood vessel growth in ischemic tissues. Various metabolicdisorders including hypeglycemia may have negative effect on therapeutic properties of these cells. Aim. To study the influence of high glucose concentration on functional activity in human adipose tissue. Materials and methods. Flow cytometry and real time PCR were used to study functional activity of cultured MSC from human adipose issue at highglucose concentration. Results. Prolonged (10-12 days) incubation at a high glucose concentration (25 mM) suppressed the ability of MSC to stimulate angiogenesis. Also,glucose modified expression of genes activating and inhibiting angiogenesis but had no effect on MSC proliferation and apoptosis. Conclusion. High glucose concentration suppresses angiogenic activity of MSC in adipose tissue; it may account for incomplete restoration of bloodflow in diabetic patients. |
| format | Article |
| id | doaj-art-549fadb525d940bd80e45d1ac7c24ce9 |
| institution | DOAJ |
| issn | 2072-0351 2072-0378 |
| language | English |
| publishDate | 2011-06-01 |
| publisher | Endocrinology Research Centre |
| record_format | Article |
| series | Сахарный диабет |
| spelling | doaj-art-549fadb525d940bd80e45d1ac7c24ce92025-08-20T03:09:01ZengEndocrinology Research CentreСахарный диабет2072-03512072-03782011-06-01142323510.14341/2072-0351-56315589The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growthZhanna Alekseevna Akopyan0Georgy Vladimirovich Sharonov1Tatiana Nikolaevna Kochegura2Natalya Fedorovna Il'yashenko3Igor Eduardovich Belyanko4Valentina Ivanovna Dimitrova5Andrey Evgen'evich Zotikov6Natalya Igorevna Kalinina7Elena Viktorovna Parfenova8Department of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowDepartment of Fundamental Medicine, M.V.Lomonosov Moscow State University, MoscowAdipose issue is a source of mesenchymal stem cells (MSC) that can be used to stimulate blood vessel growth in ischemic tissues. Various metabolicdisorders including hypeglycemia may have negative effect on therapeutic properties of these cells. Aim. To study the influence of high glucose concentration on functional activity in human adipose tissue. Materials and methods. Flow cytometry and real time PCR were used to study functional activity of cultured MSC from human adipose issue at highglucose concentration. Results. Prolonged (10-12 days) incubation at a high glucose concentration (25 mM) suppressed the ability of MSC to stimulate angiogenesis. Also,glucose modified expression of genes activating and inhibiting angiogenesis but had no effect on MSC proliferation and apoptosis. Conclusion. High glucose concentration suppresses angiogenic activity of MSC in adipose tissue; it may account for incomplete restoration of bloodflow in diabetic patients.https://www.dia-endojournals.ru/jour/article/view/5631hyperglycemiaangiogenesismesenchymal stem cellsgrowth factors |
| spellingShingle | Zhanna Alekseevna Akopyan Georgy Vladimirovich Sharonov Tatiana Nikolaevna Kochegura Natalya Fedorovna Il'yashenko Igor Eduardovich Belyanko Valentina Ivanovna Dimitrova Andrey Evgen'evich Zotikov Natalya Igorevna Kalinina Elena Viktorovna Parfenova The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth Сахарный диабет hyperglycemia angiogenesis mesenchymal stem cells growth factors |
| title | The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth |
| title_full | The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth |
| title_fullStr | The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth |
| title_full_unstemmed | The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth |
| title_short | The influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth |
| title_sort | influence of high glucose concentration on the ability of mesenchymal stromal cells to stimulate blood vessel growth |
| topic | hyperglycemia angiogenesis mesenchymal stem cells growth factors |
| url | https://www.dia-endojournals.ru/jour/article/view/5631 |
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