Using the Sleeping Beauty Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis
Macrophages are known for engulfing and digesting pathogens and dead cells through a specialized form of endocytosis called phagocytosis. Unfortunately, many macrophage cell lines are refractory to most reagents used for transient transfections. Alternative transient approaches, such as electroporat...
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Main Authors: | Parsa Kamali, Gregory Fairn |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2025-02-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=5178&type=0 |
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