Using the Sleeping Beauty Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis

Macrophages are known for engulfing and digesting pathogens and dead cells through a specialized form of endocytosis called phagocytosis. Unfortunately, many macrophage cell lines are refractory to most reagents used for transient transfections. Alternative transient approaches, such as electroporat...

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Bibliographic Details
Main Authors: Parsa Kamali, Gregory Fairn
Format: Article
Language:English
Published: Bio-protocol LLC 2025-02-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5178&type=0
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