Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic Dogs
We used terminal restriction fragment polymorphism (T-RFLP) analysis to assess (1) stability of the fecal microbiota in dogs living in environments characterized by varying degrees of exposure to factors that might alter the microbiota and (2) changes in the microbiota associated with acute episodes...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Interdisciplinary Perspectives on Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/2008/149694 |
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| author | Julia A. Bell Jamie J. Kopper Judy A. Turnbull Nicholas I. Barbu Alice J. Murphy Linda S. Mansfield |
| author_facet | Julia A. Bell Jamie J. Kopper Judy A. Turnbull Nicholas I. Barbu Alice J. Murphy Linda S. Mansfield |
| author_sort | Julia A. Bell |
| collection | DOAJ |
| description | We used terminal restriction fragment polymorphism (T-RFLP) analysis to assess (1) stability of the fecal microbiota in dogs living in environments characterized by varying degrees of exposure to factors that might alter the microbiota and (2) changes in the microbiota associated with acute episodes of diarrhea. Results showed that the healthy canine GI tract harbors potential enteric pathogens. Dogs living in an environment providing minimal exposure to factors that might alter the microbiota had similar microbiotas; the microbiotas of dogs kept in more variable environments were more variable. Substantial changes in the microbiota occurred during diarrheic episodes, including increased levels of Clostridium perfringens, Enterococcus faecalis, and Enterococcus faecium. When diet and medications of a dog having a previously stable microbiota were changed repeatedly, the microbiota also changed repeatedly. Temporal trend analysis showed directional changes in the microbiota after perturbation, a return to the starting condition, and then fluctuating changes over time. |
| format | Article |
| id | doaj-art-549dd0ff9a554ce59ccdf4ad7f5b9980 |
| institution | Kabale University |
| issn | 1687-708X 1687-7098 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Interdisciplinary Perspectives on Infectious Diseases |
| spelling | doaj-art-549dd0ff9a554ce59ccdf4ad7f5b99802025-08-20T03:34:20ZengWileyInterdisciplinary Perspectives on Infectious Diseases1687-708X1687-70982008-01-01200810.1155/2008/149694149694Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic DogsJulia A. Bell0Jamie J. Kopper1Judy A. Turnbull2Nicholas I. Barbu3Alice J. Murphy4Linda S. Mansfield5Comparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, 181 Food Safety Building, East Lansing, MI 48824, USAComparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, 181 Food Safety Building, East Lansing, MI 48824, USAComparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, 181 Food Safety Building, East Lansing, MI 48824, USAComparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, 181 Food Safety Building, East Lansing, MI 48824, USAComparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, 181 Food Safety Building, East Lansing, MI 48824, USAComparative Enteric Diseases Laboratory, National Food Safety and Toxicology Center, Michigan State University, 181 Food Safety Building, East Lansing, MI 48824, USAWe used terminal restriction fragment polymorphism (T-RFLP) analysis to assess (1) stability of the fecal microbiota in dogs living in environments characterized by varying degrees of exposure to factors that might alter the microbiota and (2) changes in the microbiota associated with acute episodes of diarrhea. Results showed that the healthy canine GI tract harbors potential enteric pathogens. Dogs living in an environment providing minimal exposure to factors that might alter the microbiota had similar microbiotas; the microbiotas of dogs kept in more variable environments were more variable. Substantial changes in the microbiota occurred during diarrheic episodes, including increased levels of Clostridium perfringens, Enterococcus faecalis, and Enterococcus faecium. When diet and medications of a dog having a previously stable microbiota were changed repeatedly, the microbiota also changed repeatedly. Temporal trend analysis showed directional changes in the microbiota after perturbation, a return to the starting condition, and then fluctuating changes over time.http://dx.doi.org/10.1155/2008/149694 |
| spellingShingle | Julia A. Bell Jamie J. Kopper Judy A. Turnbull Nicholas I. Barbu Alice J. Murphy Linda S. Mansfield Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic Dogs Interdisciplinary Perspectives on Infectious Diseases |
| title | Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic Dogs |
| title_full | Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic Dogs |
| title_fullStr | Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic Dogs |
| title_full_unstemmed | Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic Dogs |
| title_short | Ecological Characterization of the Colonic Microbiota of Normal and Diarrheic Dogs |
| title_sort | ecological characterization of the colonic microbiota of normal and diarrheic dogs |
| url | http://dx.doi.org/10.1155/2008/149694 |
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