Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice
Diabetes mellitus is the most common chronic disease worldwide that causes numerous complications, including male infertility. The prevalence of DM is 451 million people and estimated that would increase to 693 million in 2045. Fluorosis caused by drinking water contaminated with inorganic fluoride...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2019/5269380 |
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| author | Manuel Sánchez-Gutiérrez Evelia Martínez-Loredo Eduardo Osiris Madrigal-Santillán Gabriel Betanzos-Cabrera Araceli Hernández-Zavala María Angélica Mojica-Villegas Jeannett Alejandra Izquierdo-Vega |
| author_facet | Manuel Sánchez-Gutiérrez Evelia Martínez-Loredo Eduardo Osiris Madrigal-Santillán Gabriel Betanzos-Cabrera Araceli Hernández-Zavala María Angélica Mojica-Villegas Jeannett Alejandra Izquierdo-Vega |
| author_sort | Manuel Sánchez-Gutiérrez |
| collection | DOAJ |
| description | Diabetes mellitus is the most common chronic disease worldwide that causes numerous complications, including male infertility. The prevalence of DM is 451 million people and estimated that would increase to 693 million in 2045. Fluorosis caused by drinking water contaminated with inorganic fluoride is a public health problem in many areas around the world. Previous studies have shown that fluoride exposure damages the male reproductive function. This study aimed to evaluate the fluoride sub-chronic exposure on the spermatozoa function in streptozotocin (STZ)-induced diabetic mice. After confirming diabetes by measuring blood glucose levels, the male mice received 45.2 ppm of fluoride added or deionized water. We evaluated several parameters in diabetic mice exposed to fluoride: standard quality analysis, the mitochondrial transmembrane potential (ψm), the caspase activity in spermatozoa, urinary fluoride excretion, and histological evaluation in the testes. After 60 days of fluoride-exposure, diabetic mice, significantly decreased sperm quality (motility, viability, and concentration). Spermatozoa from fluoride-exposure in diabetic mice presented a significant decrease in ψm and a significant increase in activity caspase 3/7. Urinary fluoride excretion was decreased in diabetic mice exposed to fluoride. Subchronic fluoride exposure of mice with STZ-induced diabetes aggravated testicular damage and the spermatozoa function. |
| format | Article |
| id | doaj-art-549aa44de01d4cd5b558e0e5afe5b5b9 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-549aa44de01d4cd5b558e0e5afe5b5b92025-08-20T03:34:20ZengWileyJournal of Toxicology1687-81911687-82052019-01-01201910.1155/2019/52693805269380Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in MiceManuel Sánchez-Gutiérrez0Evelia Martínez-Loredo1Eduardo Osiris Madrigal-Santillán2Gabriel Betanzos-Cabrera3Araceli Hernández-Zavala4María Angélica Mojica-Villegas5Jeannett Alejandra Izquierdo-Vega6Área Académica de Medicina, Instituto de Ciencias de la Salud, Universidad Autonoma del Estado de Hidalgo, Carretera Actopan-Tilcuautla, Ex-hacienda La Concepción S/N, San Agustín Tlaxiaca 42160, Hidalgo, MexicoÁrea Académica de Medicina, Instituto de Ciencias de la Salud, Universidad Autonoma del Estado de Hidalgo, Carretera Actopan-Tilcuautla, Ex-hacienda La Concepción S/N, San Agustín Tlaxiaca 42160, Hidalgo, MexicoÁrea Académica de Medicina, Instituto de Ciencias de la Salud, Universidad Autonoma del Estado de Hidalgo, Carretera Actopan-Tilcuautla, Ex-hacienda La Concepción S/N, San Agustín Tlaxiaca 42160, Hidalgo, MexicoTecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Campus Querétaro, Epigmenio González 500, 76130 Querétaro, MexicoSección de Investigación y Posgrado, Escuela Superior de Medicina del Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón Col, Casco de Santo Tomas, Delegación Miguel Hidalgo, 11340 Ciudad de México, MexicoLaboratorio de Toxicología de la Reproducción-Fertilidad, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av Wilfrido Massieu S/N, Colonia Adolfo López Mateos, Ciudad de México 07738, MexicoÁrea Académica de Medicina, Instituto de Ciencias de la Salud, Universidad Autonoma del Estado de Hidalgo, Carretera Actopan-Tilcuautla, Ex-hacienda La Concepción S/N, San Agustín Tlaxiaca 42160, Hidalgo, MexicoDiabetes mellitus is the most common chronic disease worldwide that causes numerous complications, including male infertility. The prevalence of DM is 451 million people and estimated that would increase to 693 million in 2045. Fluorosis caused by drinking water contaminated with inorganic fluoride is a public health problem in many areas around the world. Previous studies have shown that fluoride exposure damages the male reproductive function. This study aimed to evaluate the fluoride sub-chronic exposure on the spermatozoa function in streptozotocin (STZ)-induced diabetic mice. After confirming diabetes by measuring blood glucose levels, the male mice received 45.2 ppm of fluoride added or deionized water. We evaluated several parameters in diabetic mice exposed to fluoride: standard quality analysis, the mitochondrial transmembrane potential (ψm), the caspase activity in spermatozoa, urinary fluoride excretion, and histological evaluation in the testes. After 60 days of fluoride-exposure, diabetic mice, significantly decreased sperm quality (motility, viability, and concentration). Spermatozoa from fluoride-exposure in diabetic mice presented a significant decrease in ψm and a significant increase in activity caspase 3/7. Urinary fluoride excretion was decreased in diabetic mice exposed to fluoride. Subchronic fluoride exposure of mice with STZ-induced diabetes aggravated testicular damage and the spermatozoa function.http://dx.doi.org/10.1155/2019/5269380 |
| spellingShingle | Manuel Sánchez-Gutiérrez Evelia Martínez-Loredo Eduardo Osiris Madrigal-Santillán Gabriel Betanzos-Cabrera Araceli Hernández-Zavala María Angélica Mojica-Villegas Jeannett Alejandra Izquierdo-Vega Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice Journal of Toxicology |
| title | Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice |
| title_full | Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice |
| title_fullStr | Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice |
| title_full_unstemmed | Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice |
| title_short | Exposure of Fluoride with Streptozotocin-Induced Diabetes Aggravates Testicular Damage and Spermatozoa Parameters in Mice |
| title_sort | exposure of fluoride with streptozotocin induced diabetes aggravates testicular damage and spermatozoa parameters in mice |
| url | http://dx.doi.org/10.1155/2019/5269380 |
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