Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)

The increasing use of silver nanoparticles (AgNPs) due to its excellent antimicrobial activity in commercial products prompting concerns about their fate in the environment. The toxicity of AgNPs is mainly the result of Ag+ ions. In this study, soil was experimentally contaminated with 100 mg kg-1 o...

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Main Authors: Hartati Oktarina, Ian Singleton
Format: Article
Language:English
Published: Universitas Syiah Kuala, Chemical Engineering Department 2020-08-01
Series:Jurnal Rekayasa Kimia & Lingkungan
Subjects:
Online Access:https://jurnal.usk.ac.id/RKL/article/view/16025
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author Hartati Oktarina
Ian Singleton
author_facet Hartati Oktarina
Ian Singleton
author_sort Hartati Oktarina
collection DOAJ
description The increasing use of silver nanoparticles (AgNPs) due to its excellent antimicrobial activity in commercial products prompting concerns about their fate in the environment. The toxicity of AgNPs is mainly the result of Ag+ ions. In this study, soil was experimentally contaminated with 100 mg kg-1 of AgNO3 to investigate its effect on fungal soil community. Deoxyribonucleic acid (DNA) from the soil was extracted at the 6th, 12th, and 24th month of observation and assessed by Illumina Next Generation Sequencing (NGS). The results show that, the pollutant change fungal community in soil. After 12 months incubated the number of fungal species in the soil reduced significantly and 40% of the community was dominated by one species.
format Article
id doaj-art-549228fced434a7fa0a68079495e76bf
institution Kabale University
issn 1412-5064
2356-1661
language English
publishDate 2020-08-01
publisher Universitas Syiah Kuala, Chemical Engineering Department
record_format Article
series Jurnal Rekayasa Kimia & Lingkungan
spelling doaj-art-549228fced434a7fa0a68079495e76bf2025-08-20T03:50:32ZengUniversitas Syiah Kuala, Chemical Engineering DepartmentJurnal Rekayasa Kimia & Lingkungan1412-50642356-16612020-08-011529910310.23955/rkl.v15i2.1602511461Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)Hartati Oktarina0Ian Singleton1universitas Syiah KualaEdinburgh Napier University, Edinburgh, United KingdomThe increasing use of silver nanoparticles (AgNPs) due to its excellent antimicrobial activity in commercial products prompting concerns about their fate in the environment. The toxicity of AgNPs is mainly the result of Ag+ ions. In this study, soil was experimentally contaminated with 100 mg kg-1 of AgNO3 to investigate its effect on fungal soil community. Deoxyribonucleic acid (DNA) from the soil was extracted at the 6th, 12th, and 24th month of observation and assessed by Illumina Next Generation Sequencing (NGS). The results show that, the pollutant change fungal community in soil. After 12 months incubated the number of fungal species in the soil reduced significantly and 40% of the community was dominated by one species.https://jurnal.usk.ac.id/RKL/article/view/16025agnps, soil contamination, metal pollution, illumine miseq, uparse pipeline
spellingShingle Hartati Oktarina
Ian Singleton
Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)
Jurnal Rekayasa Kimia & Lingkungan
agnps, soil contamination, metal pollution, illumine miseq, uparse pipeline
title Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)
title_full Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)
title_fullStr Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)
title_full_unstemmed Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)
title_short Soil Fungal Community Responses to the Silver Nanoparticles Contamination as Assessed by Illumina Next Generation Sequencing (NGS)
title_sort soil fungal community responses to the silver nanoparticles contamination as assessed by illumina next generation sequencing ngs
topic agnps, soil contamination, metal pollution, illumine miseq, uparse pipeline
url https://jurnal.usk.ac.id/RKL/article/view/16025
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AT iansingleton soilfungalcommunityresponsestothesilvernanoparticlescontaminationasassessedbyilluminanextgenerationsequencingngs