UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice
Summary: The coordination of numerous proteins is necessary for spermatogenesis, including degradation through the ubiquitin-proteasome pathway. Ubiquitin binding enzyme E2 (UBE2J2) is involved in the degradation of endoplasmic reticulum-associated proteins, while its role in spermatogenesis remains...
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Elsevier
2025-08-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225011393 |
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| author | Xiaochen Yu Jie Cen Yaxuan Zhang Tongtong Li Mingyu Zhang Fei Gao Hongbin Liu Yongzhi Cao |
| author_facet | Xiaochen Yu Jie Cen Yaxuan Zhang Tongtong Li Mingyu Zhang Fei Gao Hongbin Liu Yongzhi Cao |
| author_sort | Xiaochen Yu |
| collection | DOAJ |
| description | Summary: The coordination of numerous proteins is necessary for spermatogenesis, including degradation through the ubiquitin-proteasome pathway. Ubiquitin binding enzyme E2 (UBE2J2) is involved in the degradation of endoplasmic reticulum-associated proteins, while its role in spermatogenesis remains unclear. We found that Ube2j2−/− mice exhibit azoospermia and spermatocytes undergo meiotic arrest at the mid-pachytene stage. Examining homologous recombination (HR) markers indicated that HR intermediate complexes are unstable and fail to form crossovers in Ube2j2−/− spermatocytes. Proteomics analysis uncovered an extensive suite of meiosis- and chromosome segregation–associated proteins unexpressed in mouse spermatocytes lacking functional UBE2J2. Our findings suggest that UBE2J2 could possibly play a key role in SC disassembly, ensuring meiosis can proceed in the late pachynema during male germline cell development in mice, and serves as an essential factor in meiotic recombination and spermatogenesis. |
| format | Article |
| id | doaj-art-548f2e95bd704167aef35f85d4c0d2f6 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-548f2e95bd704167aef35f85d4c0d2f62025-08-20T03:49:56ZengElsevieriScience2589-00422025-08-0128811287810.1016/j.isci.2025.112878UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in miceXiaochen Yu0Jie Cen1Yaxuan Zhang2Tongtong Li3Mingyu Zhang4Fei Gao5Hongbin Liu6Yongzhi Cao7Institute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong 250012, China; Center for Reproductive Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, ChinaInstitute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, ChinaInstitute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, ChinaInstitute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, ChinaInstitute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, ChinaInstitute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaInstitute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, ChinaInstitute of Women, Children and Reproductive Health, Shandong University, Jinan 250012, China; State Key Laboratory of Reproductive Medicine and Offspring Health, Shandong University, Jinan, Shandong 250012, China; National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong 250012, China; Key Laboratory of Reproductive Endocrinology (Shandong University), Ministry of Education, Jinan, Shandong 250012, China; Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong 250012, China; Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, Shandong 250012, China; Model Animal Research Center, Shandong University, Jinan, Shandong 250012, China; Corresponding authorSummary: The coordination of numerous proteins is necessary for spermatogenesis, including degradation through the ubiquitin-proteasome pathway. Ubiquitin binding enzyme E2 (UBE2J2) is involved in the degradation of endoplasmic reticulum-associated proteins, while its role in spermatogenesis remains unclear. We found that Ube2j2−/− mice exhibit azoospermia and spermatocytes undergo meiotic arrest at the mid-pachytene stage. Examining homologous recombination (HR) markers indicated that HR intermediate complexes are unstable and fail to form crossovers in Ube2j2−/− spermatocytes. Proteomics analysis uncovered an extensive suite of meiosis- and chromosome segregation–associated proteins unexpressed in mouse spermatocytes lacking functional UBE2J2. Our findings suggest that UBE2J2 could possibly play a key role in SC disassembly, ensuring meiosis can proceed in the late pachynema during male germline cell development in mice, and serves as an essential factor in meiotic recombination and spermatogenesis.http://www.sciencedirect.com/science/article/pii/S2589004225011393Molecular biologyCell biologyOrganizational aspects of cell biology |
| spellingShingle | Xiaochen Yu Jie Cen Yaxuan Zhang Tongtong Li Mingyu Zhang Fei Gao Hongbin Liu Yongzhi Cao UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice iScience Molecular biology Cell biology Organizational aspects of cell biology |
| title | UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice |
| title_full | UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice |
| title_fullStr | UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice |
| title_full_unstemmed | UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice |
| title_short | UBE2J2 is essential for the progression of meiosis prophase I during spermatogenesis in mice |
| title_sort | ube2j2 is essential for the progression of meiosis prophase i during spermatogenesis in mice |
| topic | Molecular biology Cell biology Organizational aspects of cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225011393 |
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