Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11
Background. A major challenge to malaria vaccine development is identification of protective epitopes and respective protective immune responses. Objective. To characterize naturally acquired Immunoglobulin G (IgG) responses to the synthetic peptide AS202.11, a malaria vaccine candidate. Methodology...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2017/6843701 |
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| author | Rebeka Nazareth Pius Horumpende Tolbert Sonda Arnold Ndaro Edson Mollel Eliakim Paul Emmanuel Athanase Jaffu Chilongola |
| author_facet | Rebeka Nazareth Pius Horumpende Tolbert Sonda Arnold Ndaro Edson Mollel Eliakim Paul Emmanuel Athanase Jaffu Chilongola |
| author_sort | Rebeka Nazareth |
| collection | DOAJ |
| description | Background. A major challenge to malaria vaccine development is identification of protective epitopes and respective protective immune responses. Objective. To characterize naturally acquired Immunoglobulin G (IgG) responses to the synthetic peptide AS202.11, a malaria vaccine candidate. Methodology. This community based cross-sectional study enrolled 320 participants aged 1 year and above. Demographic information was recorded through interviews. Detection of P. falciparum infection was done by microscopy, malaria rapid diagnostic test, and polymerase chain reaction. ELISA was used to detect IgG antibody. Data was analyzed using STATA. Results. The overall AS202.11 IgG seropositivity was 78.8% (73.9–82.9). Seropositivity by age categories was ≤12 years [74.3% (67.4–80.2)], 13–40 years [85.3% (76.5–91.1)], and >40 years [82.6% (68.7–91.1)]. Compared to the ≤ 12-year-old group, aORs for the other groups were 2.22 (1.14–4.32), p=0.019, and 1.87 (0.81–4.35), p=0.143, for the 13–40-year-old and >40-year-old groups, respectively. The 13–40-year-old group had more seropositive individuals compared to the ≤ 12-year-old group. Conclusion. We report a high degree of recognition of AS202.11 by IgG elicited by field P. falciparum strains, suggesting its close similarity to native P. falciparum antigens and possible suitability of the peptide as a future malaria vaccine candidate. |
| format | Article |
| id | doaj-art-548e54dfd38b470a8e112c9da3e878a6 |
| institution | Kabale University |
| issn | 1687-9686 1687-9694 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-548e54dfd38b470a8e112c9da3e878a62025-02-03T00:59:51ZengWileyJournal of Tropical Medicine1687-96861687-96942017-01-01201710.1155/2017/68437016843701Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11Rebeka Nazareth0Pius Horumpende1Tolbert Sonda2Arnold Ndaro3Edson Mollel4Eliakim Paul5Emmanuel Athanase6Jaffu Chilongola7Kilimanjaro Christian Medical University College, P.O. Box 2240, Moshi, TanzaniaKilimanjaro Christian Medical University College, P.O. Box 2240, Moshi, TanzaniaKilimanjaro Christian Medical University College, P.O. Box 2240, Moshi, TanzaniaKilimanjaro Clinical Research Institute, P.O. Box 2236, Moshi, TanzaniaKibong’oto Infectious Diseases Hospital, P.O. Box 12, Sanya Juu, Siha, TanzaniaKilimanjaro Christian Medical University College, P.O. Box 2240, Moshi, TanzaniaNewala Town Council Hospital, P.O. Box 39, Newala, TanzaniaKilimanjaro Christian Medical University College, P.O. Box 2240, Moshi, TanzaniaBackground. A major challenge to malaria vaccine development is identification of protective epitopes and respective protective immune responses. Objective. To characterize naturally acquired Immunoglobulin G (IgG) responses to the synthetic peptide AS202.11, a malaria vaccine candidate. Methodology. This community based cross-sectional study enrolled 320 participants aged 1 year and above. Demographic information was recorded through interviews. Detection of P. falciparum infection was done by microscopy, malaria rapid diagnostic test, and polymerase chain reaction. ELISA was used to detect IgG antibody. Data was analyzed using STATA. Results. The overall AS202.11 IgG seropositivity was 78.8% (73.9–82.9). Seropositivity by age categories was ≤12 years [74.3% (67.4–80.2)], 13–40 years [85.3% (76.5–91.1)], and >40 years [82.6% (68.7–91.1)]. Compared to the ≤ 12-year-old group, aORs for the other groups were 2.22 (1.14–4.32), p=0.019, and 1.87 (0.81–4.35), p=0.143, for the 13–40-year-old and >40-year-old groups, respectively. The 13–40-year-old group had more seropositive individuals compared to the ≤ 12-year-old group. Conclusion. We report a high degree of recognition of AS202.11 by IgG elicited by field P. falciparum strains, suggesting its close similarity to native P. falciparum antigens and possible suitability of the peptide as a future malaria vaccine candidate.http://dx.doi.org/10.1155/2017/6843701 |
| spellingShingle | Rebeka Nazareth Pius Horumpende Tolbert Sonda Arnold Ndaro Edson Mollel Eliakim Paul Emmanuel Athanase Jaffu Chilongola Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 Journal of Tropical Medicine |
| title | Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 |
| title_full | Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 |
| title_fullStr | Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 |
| title_full_unstemmed | Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 |
| title_short | Naturally Acquired Antibody Responses to a Synthetic Malaria Antigen AS202.11 |
| title_sort | naturally acquired antibody responses to a synthetic malaria antigen as202 11 |
| url | http://dx.doi.org/10.1155/2017/6843701 |
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