The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line
The present study was planned to investigate the potential protective properties of lycopene, an antioxidant carotenoid, on NaF applied renal cell line. NRK-52E cells were cultured under standard in vitro conditions with regular passages. 10.000 NRK-52E cells were planted in each culture platelet. C...
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| Format: | Article |
| Language: | English |
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izzet kara
2017-12-01
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| Series: | International Journal of Secondary Metabolite |
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| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/33748/377756?publisher=ijate |
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| author | Sedat Çetin |
| author_facet | Sedat Çetin |
| author_sort | Sedat Çetin |
| collection | DOAJ |
| description | The present study was planned to investigate the
potential protective properties of lycopene, an antioxidant carotenoid, on NaF
applied renal cell line. NRK-52E cells were cultured under standard in vitro conditions with
regular passages. 10.000 NRK-52E cells were planted in each culture platelet.
Cells were incubated for 24 hours at 37°C in an incubator with CO2.
After the incubation, the medium formed on the cells was removed and the
prepared NaF and lycopene solutions were added. At least 4 wells were used for
each dose. Culture vessels were incubated at 37°C in a CO2 incubator
for 6, 12, and 24 hours for transformation of non-soluble formazan crystals by
MTT stain and the MTT assay was conducted. In conclusion, it was found that low
lycopene concentrations reduced the toxic effect of NaF by 10-20%, while
lycopene increased the toxic effect of NaF synergistically in the high concentration
lycopene treated groups. Thus, it was concluded that administration of lycopene
on NaF applied renal cell line exhibited different effects based on
concentration and duration. |
| format | Article |
| id | doaj-art-54886f80722e4aa8bf00d8b3eaa5a3bf |
| institution | DOAJ |
| issn | 2148-6905 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | izzet kara |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj-art-54886f80722e4aa8bf00d8b3eaa5a3bf2025-08-20T03:18:12Zengizzet karaInternational Journal of Secondary Metabolite2148-69052017-12-0143, Special Issue 250851110.21448/ijsm.377756618The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell LineSedat ÇetinThe present study was planned to investigate the potential protective properties of lycopene, an antioxidant carotenoid, on NaF applied renal cell line. NRK-52E cells were cultured under standard in vitro conditions with regular passages. 10.000 NRK-52E cells were planted in each culture platelet. Cells were incubated for 24 hours at 37°C in an incubator with CO2. After the incubation, the medium formed on the cells was removed and the prepared NaF and lycopene solutions were added. At least 4 wells were used for each dose. Culture vessels were incubated at 37°C in a CO2 incubator for 6, 12, and 24 hours for transformation of non-soluble formazan crystals by MTT stain and the MTT assay was conducted. In conclusion, it was found that low lycopene concentrations reduced the toxic effect of NaF by 10-20%, while lycopene increased the toxic effect of NaF synergistically in the high concentration lycopene treated groups. Thus, it was concluded that administration of lycopene on NaF applied renal cell line exhibited different effects based on concentration and duration.https://dergipark.org.tr/tr/pub/ijsm/issue/33748/377756?publisher=ijatenaflycopenecell culturemttkidney. |
| spellingShingle | Sedat Çetin The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line International Journal of Secondary Metabolite naf lycopene cell culture mtt kidney. |
| title | The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line |
| title_full | The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line |
| title_fullStr | The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line |
| title_full_unstemmed | The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line |
| title_short | The Effects of Lycopene Application on Sodium Fluoride (NaF) Applied Renal Cell Line |
| title_sort | effects of lycopene application on sodium fluoride naf applied renal cell line |
| topic | naf lycopene cell culture mtt kidney. |
| url | https://dergipark.org.tr/tr/pub/ijsm/issue/33748/377756?publisher=ijate |
| work_keys_str_mv | AT sedatcetin theeffectsoflycopeneapplicationonsodiumfluoridenafappliedrenalcellline AT sedatcetin effectsoflycopeneapplicationonsodiumfluoridenafappliedrenalcellline |