Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis
The present study reports the structural characteristics of 3 polysaccharide fractions (SPS-F1, SPS- F2 and SPS-F3) isolated and purified from squash. SPS-F1 (molecular weight (Mw) = 12.30 kDa) and SPS-F2 (Mw = 19.40 kDa) were likely to contain HG and RG-I domain of pectic polysaccharide, respective...
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Tsinghua University Press
2024-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250237 |
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| author | Li Liang Fuming Zhang Quanhong Li Baoguo Sun Yuyu Zhang Robert J. Linhardt |
| author_facet | Li Liang Fuming Zhang Quanhong Li Baoguo Sun Yuyu Zhang Robert J. Linhardt |
| author_sort | Li Liang |
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| description | The present study reports the structural characteristics of 3 polysaccharide fractions (SPS-F1, SPS- F2 and SPS-F3) isolated and purified from squash. SPS-F1 (molecular weight (Mw) = 12.30 kDa) and SPS-F2 (Mw = 19.40 kDa) were likely to contain HG and RG-I domain of pectic polysaccharide, respectively. SPS-F2 (Mw = 270.4 kDa) was mainly composed of rhamnose, galactose and arabinose. The treatment with SPS decreased body weight gain, glucose and TG levels in type 2 diabetes rats. Besides, 25 differential metabolites were identified based on urinary metabolomics analysis, which are crucial to the anti-diabetic effect of SPS. The regulation of nicotinamide N-oxide, histamine, cis-aconitate, citrate, L-malic acid, 3-(3-hydroxyphenyl) propanoic acid and N-acetyl-L-aspartic acid were mainly associated with energy metabolism, gut microbiota and inflammation. Study of surface plasmon resonance revealed the binding kinetics with galectin-3 (Gal-3) and fibroblast growth factor 2 (FGF2). The KD values of SPS-F2 and SPS-F3 to Gal-3 were 4.97 × 10-3 and 1.48 × 10-3 mol/L, indicating a weak binding affinity. All 3 fractions showed moderate binding to FGF2 and the affinity was SPS-F3 > SPS-F2 > SPS-F1. Thus, the metabolomics and SPR approach were proved to be a promising tool in exploring the anti-diabetes effects of SPS and provided a deep understanding of the mechanisms. |
| format | Article |
| id | doaj-art-5483ed5be6a14e07969eeae79aee0e05 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-5483ed5be6a14e07969eeae79aee0e052025-01-10T06:56:56ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011352937294610.26599/FSHW.2022.9250237Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysisLi Liang0Fuming Zhang1Quanhong Li2Baoguo Sun3Yuyu Zhang4Robert J. Linhardt5Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University (BTBU), Beijing 100048, ChinaDepartments of Chemical and Biological Engineering, Chemistry and Chemical Biology, Biomedical Engineering and Biological Science, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy 12180, USACollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University (BTBU), Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University (BTBU), Beijing 100048, ChinaDepartments of Chemical and Biological Engineering, Chemistry and Chemical Biology, Biomedical Engineering and Biological Science, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy 12180, USAThe present study reports the structural characteristics of 3 polysaccharide fractions (SPS-F1, SPS- F2 and SPS-F3) isolated and purified from squash. SPS-F1 (molecular weight (Mw) = 12.30 kDa) and SPS-F2 (Mw = 19.40 kDa) were likely to contain HG and RG-I domain of pectic polysaccharide, respectively. SPS-F2 (Mw = 270.4 kDa) was mainly composed of rhamnose, galactose and arabinose. The treatment with SPS decreased body weight gain, glucose and TG levels in type 2 diabetes rats. Besides, 25 differential metabolites were identified based on urinary metabolomics analysis, which are crucial to the anti-diabetic effect of SPS. The regulation of nicotinamide N-oxide, histamine, cis-aconitate, citrate, L-malic acid, 3-(3-hydroxyphenyl) propanoic acid and N-acetyl-L-aspartic acid were mainly associated with energy metabolism, gut microbiota and inflammation. Study of surface plasmon resonance revealed the binding kinetics with galectin-3 (Gal-3) and fibroblast growth factor 2 (FGF2). The KD values of SPS-F2 and SPS-F3 to Gal-3 were 4.97 × 10-3 and 1.48 × 10-3 mol/L, indicating a weak binding affinity. All 3 fractions showed moderate binding to FGF2 and the affinity was SPS-F3 > SPS-F2 > SPS-F1. Thus, the metabolomics and SPR approach were proved to be a promising tool in exploring the anti-diabetes effects of SPS and provided a deep understanding of the mechanisms.https://www.sciopen.com/article/10.26599/FSHW.2022.9250237polysaccharidemetabolomicstype 2 diabetessurface plasmon resonance |
| spellingShingle | Li Liang Fuming Zhang Quanhong Li Baoguo Sun Yuyu Zhang Robert J. Linhardt Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis Food Science and Human Wellness polysaccharide metabolomics type 2 diabetes surface plasmon resonance |
| title | Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis |
| title_full | Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis |
| title_fullStr | Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis |
| title_full_unstemmed | Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis |
| title_short | Characterization of squash polysaccharide and the anti-diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis |
| title_sort | characterization of squash polysaccharide and the anti diabetic effect on type 2 diabetic rat revealed by urine metabolomics analysis |
| topic | polysaccharide metabolomics type 2 diabetes surface plasmon resonance |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250237 |
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