The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombucha

This research aimed to determine the effects of green tea and black tea on the total acid content, alcohol concentration, and total phenolic content of kombucha. The fermentation process was carried out over 14 days to monitor changes in key characteristics, including total titrated acid, pH, reduci...

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Main Authors: Lembono Pilandari, Firdaus Lieneilson, Tanuwidjaja Kornelius Sophiano
Format: Article
Language:English
Published: EDP Sciences 2025-01-01
Series:BIO Web of Conferences
Subjects:
Online Access:https://www.bio-conferences.org/articles/bioconf/pdf/2025/20/bioconf_isofst2025_01001.pdf
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author Lembono Pilandari
Firdaus Lieneilson
Tanuwidjaja Kornelius Sophiano
author_facet Lembono Pilandari
Firdaus Lieneilson
Tanuwidjaja Kornelius Sophiano
author_sort Lembono Pilandari
collection DOAJ
description This research aimed to determine the effects of green tea and black tea on the total acid content, alcohol concentration, and total phenolic content of kombucha. The fermentation process was carried out over 14 days to monitor changes in key characteristics, including total titrated acid, pH, reducing sugar content, alcohol content, and Total Polyphenol Content (TPC). The results indicated that kombucha made from green tea contained higher alcohol levels and greater phenolic content, whereas kombucha from black tea had a higher acid concentration. By the end of the 14th day of fermentation, the acid content of green tea kombucha reached 0.240 M with a pH of 2.56, while the black tea variant reached 0.246 M with a pH of 2.61. Additionally, the alcohol content peaked on day 14 for both green tea and black tea, at 0.84% and 0.52% (v/v), respectively. The highest Total Polyphenol Content for green tea was recorded on day 12, measuring 344.4 mg/L, while black tea reached its peak of 333.89 mg/L on day 7. Based on the organoleptic tests and the analysis of kombucha contents, the optimal fermentation time for both green tea and black tea kombucha is concluded to be 7 days.
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spelling doaj-art-5472bb5a8f274c469060c98587d5d1362025-08-20T03:42:23ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011690100110.1051/bioconf/202516901001bioconf_isofst2025_01001The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombuchaLembono Pilandari0Firdaus Lieneilson1Tanuwidjaja Kornelius Sophiano2Calvin Institute of Technology, Chemical and Food Processing DepartmentCalvin Institute of Technology, Chemical and Food Processing DepartmentCalvin Institute of Technology, Chemical and Food Processing DepartmentThis research aimed to determine the effects of green tea and black tea on the total acid content, alcohol concentration, and total phenolic content of kombucha. The fermentation process was carried out over 14 days to monitor changes in key characteristics, including total titrated acid, pH, reducing sugar content, alcohol content, and Total Polyphenol Content (TPC). The results indicated that kombucha made from green tea contained higher alcohol levels and greater phenolic content, whereas kombucha from black tea had a higher acid concentration. By the end of the 14th day of fermentation, the acid content of green tea kombucha reached 0.240 M with a pH of 2.56, while the black tea variant reached 0.246 M with a pH of 2.61. Additionally, the alcohol content peaked on day 14 for both green tea and black tea, at 0.84% and 0.52% (v/v), respectively. The highest Total Polyphenol Content for green tea was recorded on day 12, measuring 344.4 mg/L, while black tea reached its peak of 333.89 mg/L on day 7. Based on the organoleptic tests and the analysis of kombucha contents, the optimal fermentation time for both green tea and black tea kombucha is concluded to be 7 days.https://www.bio-conferences.org/articles/bioconf/pdf/2025/20/bioconf_isofst2025_01001.pdfalcoholkombuchapolyphenoltotal acid
spellingShingle Lembono Pilandari
Firdaus Lieneilson
Tanuwidjaja Kornelius Sophiano
The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombucha
BIO Web of Conferences
alcohol
kombucha
polyphenol
total acid
title The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombucha
title_full The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombucha
title_fullStr The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombucha
title_full_unstemmed The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombucha
title_short The effects of green tea and black tea on the total acid, alcohol content, and antioxidant levels in kombucha
title_sort effects of green tea and black tea on the total acid alcohol content and antioxidant levels in kombucha
topic alcohol
kombucha
polyphenol
total acid
url https://www.bio-conferences.org/articles/bioconf/pdf/2025/20/bioconf_isofst2025_01001.pdf
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