Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous Urine
A complex mixture of peptides plays a key role in the regulation of the immune system; different sources as raw materials mainly from animals and vegetables have been reported to provide these extracts. The batch-to-batch product consistency depends on in-process controls established. However, when...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/3683782 |
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| author | Alberto Fragoso Mérida Pedraza-Jiménez Laura Espinoza-González María Luisa Ceja-Mendoza Hugo Sánchez-Mercado Gloria Robles-Pérez Julio Granados Emilio Medina-Rivero |
| author_facet | Alberto Fragoso Mérida Pedraza-Jiménez Laura Espinoza-González María Luisa Ceja-Mendoza Hugo Sánchez-Mercado Gloria Robles-Pérez Julio Granados Emilio Medina-Rivero |
| author_sort | Alberto Fragoso |
| collection | DOAJ |
| description | A complex mixture of peptides plays a key role in the regulation of the immune system; different sources as raw materials mainly from animals and vegetables have been reported to provide these extracts. The batch-to-batch product consistency depends on in-process controls established. However, when an immunomodulator is a customized product obtained from the same volunteer who will receive the product to personalize the treatment, the criteria to establish the consistency between volunteers are different. In this sense, it is expected to have the same molecular weight range although the profile of peptide abundance is different. Here, we characterized the peptide profile of three extracts of an immunomodulator obtained from the urine of different volunteers suffering from three different diseases (i.e., allergic rhinitis, rheumatoid arthritis, and chronic rhinopharyngitis), using size exclusion chromatography (SEC) and mass spectrometry (MS). The peptides contained in the immunomodulators were stable after six months, stored in a refrigerator. Our results showed a chromatographic profile with the same range of low molecular weight (less than 17 kDa) in all analyzed samples by SEC; these results were also confirmed by MS showing an exact mass spectrum from 3 to 13 kDa. The fact that the peptide profiles were conserved during a six-month period at refrigeration conditions (2 to 8°C) maintaining the quality and stability of the immunomodulator supports the notion that it might be an alternative in the treatment of chronic hypersensibility disorders. |
| format | Article |
| id | doaj-art-5472bb1c95344cba8e15ba4fc5948d31 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-5472bb1c95344cba8e15ba4fc5948d312025-02-03T01:26:58ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/36837823683782Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous UrineAlberto Fragoso0Mérida Pedraza-Jiménez1Laura Espinoza-González2María Luisa Ceja-Mendoza3Hugo Sánchez-Mercado4Gloria Robles-Pérez5Julio Granados6Emilio Medina-Rivero7Instituto de Alergias y Autoinmunidad Dr. Maximiliano Ruiz Castañeda A.C., Luisa Isabel Campos #16, col. Revolución, Acambay, Edo. de México 50300, MexicoInstituto de Alergias y Autoinmunidad Dr. Maximiliano Ruiz Castañeda A.C., Luisa Isabel Campos #16, col. Revolución, Acambay, Edo. de México 50300, MexicoInstituto de Alergias y Autoinmunidad Dr. Maximiliano Ruiz Castañeda A.C., Luisa Isabel Campos #16, col. Revolución, Acambay, Edo. de México 50300, MexicoInstituto de Alergias y Autoinmunidad Dr. Maximiliano Ruiz Castañeda A.C., Luisa Isabel Campos #16, col. Revolución, Acambay, Edo. de México 50300, MexicoInstituto de Alergias y Autoinmunidad Dr. Maximiliano Ruiz Castañeda A.C., Luisa Isabel Campos #16, col. Revolución, Acambay, Edo. de México 50300, MexicoInstituto de Alergias y Autoinmunidad Dr. Maximiliano Ruiz Castañeda A.C., Luisa Isabel Campos #16, col. Revolución, Acambay, Edo. de México 50300, MexicoInstituto de Alergias y Autoinmunidad Dr. Maximiliano Ruiz Castañeda A.C., Luisa Isabel Campos #16, col. Revolución, Acambay, Edo. de México 50300, MexicoUnidad de Desarrollo e Investigación en Bioprocesos (UDIBI), Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México 11340, MexicoA complex mixture of peptides plays a key role in the regulation of the immune system; different sources as raw materials mainly from animals and vegetables have been reported to provide these extracts. The batch-to-batch product consistency depends on in-process controls established. However, when an immunomodulator is a customized product obtained from the same volunteer who will receive the product to personalize the treatment, the criteria to establish the consistency between volunteers are different. In this sense, it is expected to have the same molecular weight range although the profile of peptide abundance is different. Here, we characterized the peptide profile of three extracts of an immunomodulator obtained from the urine of different volunteers suffering from three different diseases (i.e., allergic rhinitis, rheumatoid arthritis, and chronic rhinopharyngitis), using size exclusion chromatography (SEC) and mass spectrometry (MS). The peptides contained in the immunomodulators were stable after six months, stored in a refrigerator. Our results showed a chromatographic profile with the same range of low molecular weight (less than 17 kDa) in all analyzed samples by SEC; these results were also confirmed by MS showing an exact mass spectrum from 3 to 13 kDa. The fact that the peptide profiles were conserved during a six-month period at refrigeration conditions (2 to 8°C) maintaining the quality and stability of the immunomodulator supports the notion that it might be an alternative in the treatment of chronic hypersensibility disorders.http://dx.doi.org/10.1155/2020/3683782 |
| spellingShingle | Alberto Fragoso Mérida Pedraza-Jiménez Laura Espinoza-González María Luisa Ceja-Mendoza Hugo Sánchez-Mercado Gloria Robles-Pérez Julio Granados Emilio Medina-Rivero Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous Urine Journal of Immunology Research |
| title | Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous Urine |
| title_full | Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous Urine |
| title_fullStr | Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous Urine |
| title_full_unstemmed | Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous Urine |
| title_short | Characterization of a Complex Mixture of Immunomodulator Peptides Obtained from Autologous Urine |
| title_sort | characterization of a complex mixture of immunomodulator peptides obtained from autologous urine |
| url | http://dx.doi.org/10.1155/2020/3683782 |
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