Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis
Introduction: Trichomoniasis is a worldwide sexually transmitted disease caused by Trichomonas vaginalis. It inflicts severe complications to the human genitourinary system. The devastating negative effects and the emergence of resistance to common medication impose the search for safer and effecti...
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The Journal of Infection in Developing Countries
2020-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11580 |
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| author | Monira A Selim Eman M Fawzy Eman M Abd El-Rahman Reem S Abdel Hady Mohamed S Badr Enas F Abdel Hamed |
| author_facet | Monira A Selim Eman M Fawzy Eman M Abd El-Rahman Reem S Abdel Hady Mohamed S Badr Enas F Abdel Hamed |
| author_sort | Monira A Selim |
| collection | DOAJ |
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Introduction: Trichomoniasis is a worldwide sexually transmitted disease caused by Trichomonas vaginalis. It inflicts severe complications to the human genitourinary system. The devastating negative effects and the emergence of resistance to common medication impose the search for safer and effective alternatives. This research aimed to investigate the effect of the Allium sativum, Nigella sativa crude extracts (NsCE) and the combination between their most effective doses with metronidazole.
Methodology: Vaginal swabs were obtained from symptomatic patients, and cultured on Diamond's medium. Assessment of various concentrations of these herbs at different follow-up periods was done by counting the number of dead T. vaginalis trophozoites using the hemocytometer and trypan blue staining. Transmission electron microscope study was done.
Results: NsCE 9 mg/mL yielded the highest lethal effect on T. vaginalis trophozoites after 72 hours, compared with metronidazole. Combination of NsCE 9 mg/mL and metronidazole 50 µg/mL gave the best result. Additionally, Tomex90 µg/mL, represents a tolerable effect after 72 hours, but metronidazole 100 µg/mL still has higher effect. These results were confirmed by the ultrastructural changes observed in T. vaginalis trophozoites, signifying severe damage of nucleus and cytoplasm with large vacuolization and cell membrane defects.
Conclusions: NsCE is a promising anti-Trichomonas especially its combination with metronidazole which showed a high synergistic effect.
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| format | Article |
| id | doaj-art-547089d9292d4e7da5cbef46ed4ff37f |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2020-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-547089d9292d4e7da5cbef46ed4ff37f2025-08-20T02:27:09ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-07-01140710.3855/jidc.11580Evaluation of the effect of some medicinal plants on cultured Trichomonas VaginalisMonira A Selim0Eman M Fawzy1Eman M Abd El-Rahman2Reem S Abdel Hady3Mohamed S Badr4Enas F Abdel Hamed5Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, EgyptDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, EgyptDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, EgyptDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, EgyptDepartment of Obstetrics and Gynecology, Faculty of Medicine, Zagazig University, Zagazig, EgyptDepartment of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt Introduction: Trichomoniasis is a worldwide sexually transmitted disease caused by Trichomonas vaginalis. It inflicts severe complications to the human genitourinary system. The devastating negative effects and the emergence of resistance to common medication impose the search for safer and effective alternatives. This research aimed to investigate the effect of the Allium sativum, Nigella sativa crude extracts (NsCE) and the combination between their most effective doses with metronidazole. Methodology: Vaginal swabs were obtained from symptomatic patients, and cultured on Diamond's medium. Assessment of various concentrations of these herbs at different follow-up periods was done by counting the number of dead T. vaginalis trophozoites using the hemocytometer and trypan blue staining. Transmission electron microscope study was done. Results: NsCE 9 mg/mL yielded the highest lethal effect on T. vaginalis trophozoites after 72 hours, compared with metronidazole. Combination of NsCE 9 mg/mL and metronidazole 50 µg/mL gave the best result. Additionally, Tomex90 µg/mL, represents a tolerable effect after 72 hours, but metronidazole 100 µg/mL still has higher effect. These results were confirmed by the ultrastructural changes observed in T. vaginalis trophozoites, signifying severe damage of nucleus and cytoplasm with large vacuolization and cell membrane defects. Conclusions: NsCE is a promising anti-Trichomonas especially its combination with metronidazole which showed a high synergistic effect. https://jidc.org/index.php/journal/article/view/11580Trichomonas VaginalisAllium sativumNigella sativa |
| spellingShingle | Monira A Selim Eman M Fawzy Eman M Abd El-Rahman Reem S Abdel Hady Mohamed S Badr Enas F Abdel Hamed Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis Journal of Infection in Developing Countries Trichomonas Vaginalis Allium sativum Nigella sativa |
| title | Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis |
| title_full | Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis |
| title_fullStr | Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis |
| title_full_unstemmed | Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis |
| title_short | Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis |
| title_sort | evaluation of the effect of some medicinal plants on cultured trichomonas vaginalis |
| topic | Trichomonas Vaginalis Allium sativum Nigella sativa |
| url | https://jidc.org/index.php/journal/article/view/11580 |
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