Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2
Using Lactobacillus delbrueckii HB49-2 with high optical purity D-lactic acid production as original strain and D-lactic acid yield as the evaluation index, the fermentation conditions of high optical purity D-lactic acid produced by L. delbrueckii HB49-2 were optimized by single factor tests and re...
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Editorial Department of China Brewing
2025-03-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-3-203.pdf |
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| author | WANG Tong, LIU Lan, LI Peng, DENG Tao, YUE Siyuan, NIE Junhui, HUANG Xiaoping |
| author_facet | WANG Tong, LIU Lan, LI Peng, DENG Tao, YUE Siyuan, NIE Junhui, HUANG Xiaoping |
| author_sort | WANG Tong, LIU Lan, LI Peng, DENG Tao, YUE Siyuan, NIE Junhui, HUANG Xiaoping |
| collection | DOAJ |
| description | Using Lactobacillus delbrueckii HB49-2 with high optical purity D-lactic acid production as original strain and D-lactic acid yield as the evaluation index, the fermentation conditions of high optical purity D-lactic acid produced by L. delbrueckii HB49-2 were optimized by single factor tests and response surface tests, and the optical purity of D-lactic acid was calculated. The results showed that the optimal fermentation conditions for the production of D-lactic acid by L. delbrueckii HB49-2 were inoculum 15%, fermentation temperature 39 ℃, and the optimal fermentation medium were glucose 110 g/L, peptone 10 g/L, yeast extract 10 g/L, dipotassium hydrogen phosphate 0.25 g/L, potassium dihydrogen phosphate 0.25 g/L, sodium acetate 0.05 g/L, magnesium sulfate 0.5 g/L, vitamin B<sub>12</sub> 40 mg/L, and calcium carbonate 50 g/L. After 72 h of cultivation under these optimal conditions, the D-lactic acid yield was 82.15 g/L, which was 32.4% higher than that before optimization (62.01 g/L), and the average optical purity reached 99.89%. |
| format | Article |
| id | doaj-art-546bbfb4fb1c4948b56cc080b8ce4c25 |
| institution | OA Journals |
| issn | 0254-5071 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-546bbfb4fb1c4948b56cc080b8ce4c252025-08-20T02:19:44ZengEditorial Department of China BrewingZhongguo niangzao0254-50712025-03-0144320320710.11882/j.issn.0254-5071.2025.03.030Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2WANG Tong, LIU Lan, LI Peng, DENG Tao, YUE Siyuan, NIE Junhui, HUANG Xiaoping0Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang 330096, ChinaUsing Lactobacillus delbrueckii HB49-2 with high optical purity D-lactic acid production as original strain and D-lactic acid yield as the evaluation index, the fermentation conditions of high optical purity D-lactic acid produced by L. delbrueckii HB49-2 were optimized by single factor tests and response surface tests, and the optical purity of D-lactic acid was calculated. The results showed that the optimal fermentation conditions for the production of D-lactic acid by L. delbrueckii HB49-2 were inoculum 15%, fermentation temperature 39 ℃, and the optimal fermentation medium were glucose 110 g/L, peptone 10 g/L, yeast extract 10 g/L, dipotassium hydrogen phosphate 0.25 g/L, potassium dihydrogen phosphate 0.25 g/L, sodium acetate 0.05 g/L, magnesium sulfate 0.5 g/L, vitamin B<sub>12</sub> 40 mg/L, and calcium carbonate 50 g/L. After 72 h of cultivation under these optimal conditions, the D-lactic acid yield was 82.15 g/L, which was 32.4% higher than that before optimization (62.01 g/L), and the average optical purity reached 99.89%.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-3-203.pdflactobacillus delbrueckii|d-lactic acid|high optical purity|fermentation condition|medium|response surface methodology |
| spellingShingle | WANG Tong, LIU Lan, LI Peng, DENG Tao, YUE Siyuan, NIE Junhui, HUANG Xiaoping Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2 Zhongguo niangzao lactobacillus delbrueckii|d-lactic acid|high optical purity|fermentation condition|medium|response surface methodology |
| title | Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2 |
| title_full | Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2 |
| title_fullStr | Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2 |
| title_full_unstemmed | Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2 |
| title_short | Optimization of fermentation conditions for production of D-lactic acid with high optical purity by Lactobacillus delbrueckii HB49-2 |
| title_sort | optimization of fermentation conditions for production of d lactic acid with high optical purity by lactobacillus delbrueckii hb49 2 |
| topic | lactobacillus delbrueckii|d-lactic acid|high optical purity|fermentation condition|medium|response surface methodology |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-3-203.pdf |
| work_keys_str_mv | AT wangtongliulanlipengdengtaoyuesiyuanniejunhuihuangxiaoping optimizationoffermentationconditionsforproductionofdlacticacidwithhighopticalpuritybylactobacillusdelbrueckiihb492 |