Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal study
Antibiotic resistance genes (ARGs) have become one of the threats to human health worldwide. In this study, SmartChip real-time fluorescent quantitative PCR and qPCR was employed to quantify the ARGs and mobile genetic elements (MGEs) at 24 sampling sites of western Hainan. High-throughput sequencin...
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KeAi Communications Co., Ltd.
2025-12-01
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| Series: | Emerging Contaminants |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405665025000848 |
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| author | Chi Yang Yongqiang Qin Yingying Zhang Ardavan Farhadi Sai Wang Xiaoping Diao Jia Xie |
| author_facet | Chi Yang Yongqiang Qin Yingying Zhang Ardavan Farhadi Sai Wang Xiaoping Diao Jia Xie |
| author_sort | Chi Yang |
| collection | DOAJ |
| description | Antibiotic resistance genes (ARGs) have become one of the threats to human health worldwide. In this study, SmartChip real-time fluorescent quantitative PCR and qPCR was employed to quantify the ARGs and mobile genetic elements (MGEs) at 24 sampling sites of western Hainan. High-throughput sequencing of 16S rRNA was utilized to analyze the structure of microbial communities. The correlation among ARGs, environmental factors, MGEs, and microbial diversity was analyzed to elucidate the distribution, possible sources, and potential hosts of ARGs during the dry and rainy seasons. The results showed that 122 ARG species with a 93.85 % detection rate and 13 MGE species with a 100 % detection in the inshore waters of western Hainan during the dry and rainy seasons. The absolute abundance of ARGs and MGEs was higher during the dry seasons compared to the rainy seasons. Among these, aminoglycoside resistance genes exhibited the highest absolute abundance during the dry season, followed by MLSB resistance genes, while peptide resistance genes demonstrated the lowest abundance. During the rainy season, the absolute abundance of aminoglycoside resistance genes is the highest, followed by sulfonamides resistance genes, while peptide resistance genes remain the least abundant. ARGs were significantly correlated with MGEs, suggesting that MGEs may mediate the horizontal transfer and spread of ARGs. Environmental factor analyses showed that Temperature, dissolved oxygen, and total phosphorus play a key role in affecting the spatial distribution of ARGs. At the phylum level, Proteobacteria, Actinobacteria, and Bacteroidetes are the core microorganisms during the dry and rainy seasons. |
| format | Article |
| id | doaj-art-5459208a1e2f4bdda4a32c6f16fd051f |
| institution | Kabale University |
| issn | 2405-6650 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Emerging Contaminants |
| spelling | doaj-art-5459208a1e2f4bdda4a32c6f16fd051f2025-08-23T04:48:45ZengKeAi Communications Co., Ltd.Emerging Contaminants2405-66502025-12-0111410055010.1016/j.emcon.2025.100550Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal studyChi Yang0Yongqiang Qin1Yingying Zhang2Ardavan Farhadi3Sai Wang4Xiaoping Diao5Jia Xie6Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, School of Marine Biology and Fisheries, Hainan University, Haikou, Hainan, 570228, ChinaMinistry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, School of Marine Biology and Fisheries, Hainan University, Haikou, Hainan, 570228, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, School of Marine Biology and Fisheries, Hainan University, Haikou, Hainan, 570228, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, ChinaKey Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, School of Marine Biology and Fisheries, Hainan University, Haikou, Hainan, 570228, China; Corresponding author.Antibiotic resistance genes (ARGs) have become one of the threats to human health worldwide. In this study, SmartChip real-time fluorescent quantitative PCR and qPCR was employed to quantify the ARGs and mobile genetic elements (MGEs) at 24 sampling sites of western Hainan. High-throughput sequencing of 16S rRNA was utilized to analyze the structure of microbial communities. The correlation among ARGs, environmental factors, MGEs, and microbial diversity was analyzed to elucidate the distribution, possible sources, and potential hosts of ARGs during the dry and rainy seasons. The results showed that 122 ARG species with a 93.85 % detection rate and 13 MGE species with a 100 % detection in the inshore waters of western Hainan during the dry and rainy seasons. The absolute abundance of ARGs and MGEs was higher during the dry seasons compared to the rainy seasons. Among these, aminoglycoside resistance genes exhibited the highest absolute abundance during the dry season, followed by MLSB resistance genes, while peptide resistance genes demonstrated the lowest abundance. During the rainy season, the absolute abundance of aminoglycoside resistance genes is the highest, followed by sulfonamides resistance genes, while peptide resistance genes remain the least abundant. ARGs were significantly correlated with MGEs, suggesting that MGEs may mediate the horizontal transfer and spread of ARGs. Environmental factor analyses showed that Temperature, dissolved oxygen, and total phosphorus play a key role in affecting the spatial distribution of ARGs. At the phylum level, Proteobacteria, Actinobacteria, and Bacteroidetes are the core microorganisms during the dry and rainy seasons.http://www.sciencedirect.com/science/article/pii/S2405665025000848Antibiotic resistance geneMicrobial diversityNetwork analysisInshore seawaters |
| spellingShingle | Chi Yang Yongqiang Qin Yingying Zhang Ardavan Farhadi Sai Wang Xiaoping Diao Jia Xie Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal study Emerging Contaminants Antibiotic resistance gene Microbial diversity Network analysis Inshore seawaters |
| title | Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal study |
| title_full | Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal study |
| title_fullStr | Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal study |
| title_full_unstemmed | Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal study |
| title_short | Distribution and occurrence of antibiotic resistance genes and microbial diversity in western Hainan's inshore seawaters: A seasonal study |
| title_sort | distribution and occurrence of antibiotic resistance genes and microbial diversity in western hainan s inshore seawaters a seasonal study |
| topic | Antibiotic resistance gene Microbial diversity Network analysis Inshore seawaters |
| url | http://www.sciencedirect.com/science/article/pii/S2405665025000848 |
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