Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated
To confirm the characteristics and function of P<sub>38</sub> in the viral particles movement, the cDNA fragment of P<sub>38</sub> gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA<sub>2</sub> seq...
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| Language: | English |
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Zhejiang University Press
2003-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.01.0034 |
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| author | LI Hong-ye CHEN Li-geng ZHOU Xue-ping |
| author_facet | LI Hong-ye CHEN Li-geng ZHOU Xue-ping |
| author_sort | LI Hong-ye |
| collection | DOAJ |
| description | To confirm the characteristics and function of P<sub>38</sub> in the viral particles movement, the cDNA fragment of P<sub>38</sub> gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA<sub>2</sub> sequence of GFLV-F13 and was then inserted into pGEM-T-easy vector. The result of sequence analysis indicated that this gene contained 1044 nucleotides encoding 347 amino acids and were 90% and 96% identical to GFLV-F13 at nucleotide and amino acid level, respectively. Multiple sequence alignment of amino acid sequence showed that homology between P<sub>38</sub> gene of GFLV-H and that of Arabis mosaic virus (ArMV) and that of Tomato ringspot virus(TomRSV) was 85% and 38.6%, respectively, while its homology with Tabacco black ring virus(TBLV), Grapevine chrome mosaic virus (GCMV) and Raspberry ringspot virus (RRSV) was below 30%. |
| format | Article |
| id | doaj-art-5449a0b5eea549a5be9f5b128df7cfae |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-5449a0b5eea549a5be9f5b128df7cfae2025-08-20T03:34:16ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-01-0129343810.3785/1008-9209.2003.01.003410089209Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolatedLI Hong-yeCHEN Li-gengZHOU Xue-pingTo confirm the characteristics and function of P<sub>38</sub> in the viral particles movement, the cDNA fragment of P<sub>38</sub> gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA<sub>2</sub> sequence of GFLV-F13 and was then inserted into pGEM-T-easy vector. The result of sequence analysis indicated that this gene contained 1044 nucleotides encoding 347 amino acids and were 90% and 96% identical to GFLV-F13 at nucleotide and amino acid level, respectively. Multiple sequence alignment of amino acid sequence showed that homology between P<sub>38</sub> gene of GFLV-H and that of Arabis mosaic virus (ArMV) and that of Tomato ringspot virus(TomRSV) was 85% and 38.6%, respectively, while its homology with Tabacco black ring virus(TBLV), Grapevine chrome mosaic virus (GCMV) and Raspberry ringspot virus (RRSV) was below 30%.https://www.academax.com/doi/10.3785/1008-9209.2003.01.0034Hangzhou isolate of <italic>Grapevine fanleaf virus</italic>P<sub>38</sub> proteingene cloningsequence alignment |
| spellingShingle | LI Hong-ye CHEN Li-geng ZHOU Xue-ping Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated 浙江大学学报. 农业与生命科学版 Hangzhou isolate of <italic>Grapevine fanleaf virus</italic> P<sub>38</sub> protein gene cloning sequence alignment |
| title | Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated |
| title_full | Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated |
| title_fullStr | Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated |
| title_full_unstemmed | Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated |
| title_short | Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated |
| title_sort | cloning and sequence analysis of p sub 38 sub gene of grapevine fanleaf virus hangzhou isolated |
| topic | Hangzhou isolate of <italic>Grapevine fanleaf virus</italic> P<sub>38</sub> protein gene cloning sequence alignment |
| url | https://www.academax.com/doi/10.3785/1008-9209.2003.01.0034 |
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