Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated
To confirm the characteristics and function of P<sub>38</sub> in the viral particles movement, the cDNA fragment of P<sub>38</sub> gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA<sub>2</sub> seq...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.01.0034 |
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| Summary: | To confirm the characteristics and function of P<sub>38</sub> in the viral particles movement, the cDNA fragment of P<sub>38</sub> gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA<sub>2</sub> sequence of GFLV-F13 and was then inserted into pGEM-T-easy vector. The result of sequence analysis indicated that this gene contained 1044 nucleotides encoding 347 amino acids and were 90% and 96% identical to GFLV-F13 at nucleotide and amino acid level, respectively. Multiple sequence alignment of amino acid sequence showed that homology between P<sub>38</sub> gene of GFLV-H and that of Arabis mosaic virus (ArMV) and that of Tomato ringspot virus(TomRSV) was 85% and 38.6%, respectively, while its homology with Tabacco black ring virus(TBLV), Grapevine chrome mosaic virus (GCMV) and Raspberry ringspot virus (RRSV) was below 30%. |
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| ISSN: | 1008-9209 2097-5155 |