A bacterial dual positive and negative selection system for dCas9 activity.
The engineering of switchable or activatable dCas9 proteins would benefit from a single system for both positive and negative selection of dCas9 activity. Most systems that are used to interrogate dCas9 libraries use a fluorescent protein screen or an antibiotic selection for active dCas9 variants....
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| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0269270&type=printable |
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| author | Shaun Spisak Brett O'Brien Marc Ostermeier |
| author_facet | Shaun Spisak Brett O'Brien Marc Ostermeier |
| author_sort | Shaun Spisak |
| collection | DOAJ |
| description | The engineering of switchable or activatable dCas9 proteins would benefit from a single system for both positive and negative selection of dCas9 activity. Most systems that are used to interrogate dCas9 libraries use a fluorescent protein screen or an antibiotic selection for active dCas9 variants. To avoid some of the limitations of these systems, we have developed a single system capable of selecting for either active or inactive dCas9 variants. E. coli expressing active dCas9 variants are isolated in the positive selection system through growth in the presence of ampicillin. The negative selection can isolate cells lacking dCas9 activity through two separate mechanisms: growth in M9 minimal media or growth in media containing streptomycin. This system is capable of enriching for rare dCas9 variants up to 9,000-fold and possesses potential utility in directed evolution experiments to create switchable dCas9 proteins. |
| format | Article |
| id | doaj-art-54450482c9ef40349fb4200a28fa32e5 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-54450482c9ef40349fb4200a28fa32e52025-08-20T03:01:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01176e026927010.1371/journal.pone.0269270A bacterial dual positive and negative selection system for dCas9 activity.Shaun SpisakBrett O'BrienMarc OstermeierThe engineering of switchable or activatable dCas9 proteins would benefit from a single system for both positive and negative selection of dCas9 activity. Most systems that are used to interrogate dCas9 libraries use a fluorescent protein screen or an antibiotic selection for active dCas9 variants. To avoid some of the limitations of these systems, we have developed a single system capable of selecting for either active or inactive dCas9 variants. E. coli expressing active dCas9 variants are isolated in the positive selection system through growth in the presence of ampicillin. The negative selection can isolate cells lacking dCas9 activity through two separate mechanisms: growth in M9 minimal media or growth in media containing streptomycin. This system is capable of enriching for rare dCas9 variants up to 9,000-fold and possesses potential utility in directed evolution experiments to create switchable dCas9 proteins.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0269270&type=printable |
| spellingShingle | Shaun Spisak Brett O'Brien Marc Ostermeier A bacterial dual positive and negative selection system for dCas9 activity. PLoS ONE |
| title | A bacterial dual positive and negative selection system for dCas9 activity. |
| title_full | A bacterial dual positive and negative selection system for dCas9 activity. |
| title_fullStr | A bacterial dual positive and negative selection system for dCas9 activity. |
| title_full_unstemmed | A bacterial dual positive and negative selection system for dCas9 activity. |
| title_short | A bacterial dual positive and negative selection system for dCas9 activity. |
| title_sort | bacterial dual positive and negative selection system for dcas9 activity |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0269270&type=printable |
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