Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons
Human astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on leveraging reverse genetics (RG) and replicon...
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Bio-protocol LLC
2025-02-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5201&type=0 |
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| author | Hashim Ali David Noyvert Valeria Lulla |
| author_facet | Hashim Ali David Noyvert Valeria Lulla |
| author_sort | Hashim Ali |
| collection | DOAJ |
| description | Human astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on leveraging reverse genetics (RG) and replicon systems to unravel the biology of MLB genotypes, a key group of neurotropic astroviruses. Using reverse genetics and replicon systems, we identified critical genetic deletions linked to viral attenuation and neurotropism, pushing forward vaccine development. We also uncovered novel replication mechanisms involving ER membrane interactions, opening doors to new antiviral targets. Reverse genetics and replicon systems are essential for advancing our understanding of astrovirus biology, identifying virulence factors, and developing effective treatments and vaccines to combat their growing public health impact. |
| format | Article |
| id | doaj-art-5441640acb884f099b86eb6b94c78bb1 |
| institution | OA Journals |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-5441640acb884f099b86eb6b94c78bb12025-08-20T02:02:05ZengBio-protocol LLCBio-Protocol2331-83252025-02-0115410.21769/BioProtoc.5201Development and Application of MLB Human Astrovirus Reverse Genetics Clones and RepliconsHashim Ali0David Noyvert1Valeria Lulla2Department of Pathology, University of Cambridge, Cambridge, UKPresent address: Division of Infection and Immunity, University College London, London, UKDepartment of Pathology, University of Cambridge, Cambridge, UKDepartment of Pathology, University of Cambridge, Cambridge, UKHuman astroviruses pose a significant public health threat, especially to children, the elderly, and immunocompromised individuals. Nevertheless, these viruses remain largely understudied, with no approved antivirals or vaccines. This protocol focuses on leveraging reverse genetics (RG) and replicon systems to unravel the biology of MLB genotypes, a key group of neurotropic astroviruses. Using reverse genetics and replicon systems, we identified critical genetic deletions linked to viral attenuation and neurotropism, pushing forward vaccine development. We also uncovered novel replication mechanisms involving ER membrane interactions, opening doors to new antiviral targets. Reverse genetics and replicon systems are essential for advancing our understanding of astrovirus biology, identifying virulence factors, and developing effective treatments and vaccines to combat their growing public health impact.https://bio-protocol.org/en/bpdetail?id=5201&type=0 |
| spellingShingle | Hashim Ali David Noyvert Valeria Lulla Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons Bio-Protocol |
| title | Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons |
| title_full | Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons |
| title_fullStr | Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons |
| title_full_unstemmed | Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons |
| title_short | Development and Application of MLB Human Astrovirus Reverse Genetics Clones and Replicons |
| title_sort | development and application of mlb human astrovirus reverse genetics clones and replicons |
| url | https://bio-protocol.org/en/bpdetail?id=5201&type=0 |
| work_keys_str_mv | AT hashimali developmentandapplicationofmlbhumanastrovirusreversegeneticsclonesandreplicons AT davidnoyvert developmentandapplicationofmlbhumanastrovirusreversegeneticsclonesandreplicons AT valerialulla developmentandapplicationofmlbhumanastrovirusreversegeneticsclonesandreplicons |