From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona
Iron oxide magnetic nanoparticles (IONPs) are widely utilized in biomedical and industrial applications due to their unique properties, including biocompatibility, superparamagnetism, and ease of functionalization. However, their behavior in biological environments is heavily influenced by surface f...
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Elsevier
2025-10-01
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| Series: | JCIS Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666934X25000169 |
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| author | Solange Mailen Selzer Laura Vanesa Bonnet María Belén Piccoli Santiago Daniel Salas Mauricio Galiano Raquel Viviana Vico Nancy Fabiana Ferreyra |
| author_facet | Solange Mailen Selzer Laura Vanesa Bonnet María Belén Piccoli Santiago Daniel Salas Mauricio Galiano Raquel Viviana Vico Nancy Fabiana Ferreyra |
| author_sort | Solange Mailen Selzer |
| collection | DOAJ |
| description | Iron oxide magnetic nanoparticles (IONPs) are widely utilized in biomedical and industrial applications due to their unique properties, including biocompatibility, superparamagnetism, and ease of functionalization. However, their behavior in biological environments is heavily influenced by surface functionalization and the formation of the protein corona. This study investigates the impact of aryl-functionalization of iron oxide nanoparticles with carboxylic and amine groups on colloidal stability, protein corona formation, and biological interactions. The IONPs were synthesized and characterized for their physicochemical properties, including size, zeta potential, magnetic properties, and dispersibility in different media. The interaction of the nanoparticles with dipalmitoylphosphatidylcholine monolayers, as a model membrane, was evaluated. Cytotoxicity and autophagy induction were assessed in Chinese hamster ovary (CHO-K1) and cervical cancer (HeLa) cells, respectively. The results demonstrate that surface functionalization significantly alters protein corona composition, which in turn modulates nanoparticle stability, cellular uptake, and biological responses. The aryl-functionalized nanoparticles exhibited reduced interactions with cell membranes compared to unfunctionalized counterparts, and also lower autophagy induction, emphasizing the importance of surface design in minimizing adverse effects. |
| format | Article |
| id | doaj-art-54407d7954cb4c679edbb01e2fde3500 |
| institution | Kabale University |
| issn | 2666-934X |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JCIS Open |
| spelling | doaj-art-54407d7954cb4c679edbb01e2fde35002025-08-20T03:55:53ZengElsevierJCIS Open2666-934X2025-10-011910014310.1016/j.jciso.2025.100143From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein coronaSolange Mailen Selzer0Laura Vanesa Bonnet1María Belén Piccoli2Santiago Daniel Salas3Mauricio Galiano4Raquel Viviana Vico5Nancy Fabiana Ferreyra6Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Fisicoquímica, X5000HUA, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), Ciudad Universitaria, X5000HUA, Córdoba, ArgentinaUniversidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Biológica Ranwel Caputto, Ciudad Universitaria, X5000HUA, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Ciudad Universitaria, X5000HUA, Córdoba, ArgentinaUniversidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Fisicoquímica, X5000HUA, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), Ciudad Universitaria, X5000HUA, Córdoba, ArgentinaUniversidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Orgánica, X5000HUA, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), Ciudad Universitaria, X5000HUA, Córdoba, ArgentinaUniversidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Biológica Ranwel Caputto, Ciudad Universitaria, X5000HUA, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Ciudad Universitaria, X5000HUA, Córdoba, ArgentinaUniversidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Orgánica, X5000HUA, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), Ciudad Universitaria, X5000HUA, Córdoba, Argentina; Corresponding author. Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Orgánica, X5000HUA, Córdoba, Argentina.Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Fisicoquímica, X5000HUA, Córdoba, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), Ciudad Universitaria, X5000HUA, Córdoba, Argentina; Corresponding author. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Investigaciones en Fisicoquímica de Córdoba (INFIQC), Ciudad Universitaria, X5000HUA, Córdoba, Argentina.Iron oxide magnetic nanoparticles (IONPs) are widely utilized in biomedical and industrial applications due to their unique properties, including biocompatibility, superparamagnetism, and ease of functionalization. However, their behavior in biological environments is heavily influenced by surface functionalization and the formation of the protein corona. This study investigates the impact of aryl-functionalization of iron oxide nanoparticles with carboxylic and amine groups on colloidal stability, protein corona formation, and biological interactions. The IONPs were synthesized and characterized for their physicochemical properties, including size, zeta potential, magnetic properties, and dispersibility in different media. The interaction of the nanoparticles with dipalmitoylphosphatidylcholine monolayers, as a model membrane, was evaluated. Cytotoxicity and autophagy induction were assessed in Chinese hamster ovary (CHO-K1) and cervical cancer (HeLa) cells, respectively. The results demonstrate that surface functionalization significantly alters protein corona composition, which in turn modulates nanoparticle stability, cellular uptake, and biological responses. The aryl-functionalized nanoparticles exhibited reduced interactions with cell membranes compared to unfunctionalized counterparts, and also lower autophagy induction, emphasizing the importance of surface design in minimizing adverse effects.http://www.sciencedirect.com/science/article/pii/S2666934X25000169Aryl-functionalized iron oxide nanoparticlesColloidal stabilityProtein coronaAdsorption Gibbs isothermsCytotoxicityAutophagy |
| spellingShingle | Solange Mailen Selzer Laura Vanesa Bonnet María Belén Piccoli Santiago Daniel Salas Mauricio Galiano Raquel Viviana Vico Nancy Fabiana Ferreyra From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona JCIS Open Aryl-functionalized iron oxide nanoparticles Colloidal stability Protein corona Adsorption Gibbs isotherms Cytotoxicity Autophagy |
| title | From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona |
| title_full | From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona |
| title_fullStr | From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona |
| title_full_unstemmed | From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona |
| title_short | From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona |
| title_sort | from surface design to cellular response insights into aryl functionalized iron oxide nanoparticles with and without protein corona |
| topic | Aryl-functionalized iron oxide nanoparticles Colloidal stability Protein corona Adsorption Gibbs isotherms Cytotoxicity Autophagy |
| url | http://www.sciencedirect.com/science/article/pii/S2666934X25000169 |
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