Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields
Abstract Bacterial wilt caused by Ralstonia solanacearum is one of the most severe plant diseases all over the world. Currently, many scientists are using SynCom (synthetic microbial community) to control this disease. However, designing of highly efficient SynCom remains challenging. In this study,...
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| Format: | Article |
| Language: | English |
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Springer
2025-07-01
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| Series: | Applied Microbiology and Biotechnology |
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| Online Access: | https://doi.org/10.1007/s00253-025-13547-6 |
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| author | Yanxia Liu Weiqi Zhang Li Guo Han Li Jingwei Zhu Xiang Li Xiaoni Zhang Xinrun Yang Yangchun Xu Qirong Shen Tianjie Yang Zhong Wei |
| author_facet | Yanxia Liu Weiqi Zhang Li Guo Han Li Jingwei Zhu Xiang Li Xiaoni Zhang Xinrun Yang Yangchun Xu Qirong Shen Tianjie Yang Zhong Wei |
| author_sort | Yanxia Liu |
| collection | DOAJ |
| description | Abstract Bacterial wilt caused by Ralstonia solanacearum is one of the most severe plant diseases all over the world. Currently, many scientists are using SynCom (synthetic microbial community) to control this disease. However, designing of highly efficient SynCom remains challenging. In this study, we isolated 372 bacteria with different morphologies from the rhizosphere soil of healthy tobacco plants in a diseased field. Based on the antagonistic activity, compatibility, and presence of cheA gene, a marker gene of chemotaxis, we constructed a chemotactic SynCom comprising two Pseudomonas strains and one Bacillus strain. In vitro experiments revealed that vitamin C, propionic acid, and esculetin significantly promoted the growth and antagonistic ability of chemotactic SynCom strains. Next, we optimized the proportion of organic fertilizer to support the growth of the SynCom strains. The application of the bioorganic fertilizer containing chemotactic SynCom can protect diseased tobacco field from R. solanacearum invasion and enhance yields. Finally, rhizosphere bacterial community analysis showed that phyla Bacillota and Chloroflexiota were significantly enriched. This study highlights the potential of chemotactic SynCom for effective biocontrol, offering a new approach for managing bacterial wilt and enhancing crop yields. Graphical Abstract Key points • The bioorganic fertilizer containing chemotactic SynCom suppresses R. solanacearum and boosts tobacco yields. • Root exudate-selected prebiotics enhance the antagonistic effects of SynCom against pathogens. • SynCom bioorganic fertilizer regulates the rhizosphere microbiome, enriching beneficial bacterial phyla. |
| format | Article |
| id | doaj-art-54320b4e109f49ca95f6db8be240c6cb |
| institution | DOAJ |
| issn | 1432-0614 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Applied Microbiology and Biotechnology |
| spelling | doaj-art-54320b4e109f49ca95f6db8be240c6cb2025-08-20T03:04:26ZengSpringerApplied Microbiology and Biotechnology1432-06142025-07-01109111310.1007/s00253-025-13547-6Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fieldsYanxia Liu0Weiqi Zhang1Li Guo2Han Li3Jingwei Zhu4Xiang Li5Xiaoni Zhang6Xinrun Yang7Yangchun Xu8Qirong Shen9Tianjie Yang10Zhong Wei11Guizhou Academy of Tobacco ScienceJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityGuizhou Academy of Tobacco ScienceGuizhou Academy of Tobacco ScienceGuizhou Tobacco Corporation of CNTCJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityJiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural UniversityAbstract Bacterial wilt caused by Ralstonia solanacearum is one of the most severe plant diseases all over the world. Currently, many scientists are using SynCom (synthetic microbial community) to control this disease. However, designing of highly efficient SynCom remains challenging. In this study, we isolated 372 bacteria with different morphologies from the rhizosphere soil of healthy tobacco plants in a diseased field. Based on the antagonistic activity, compatibility, and presence of cheA gene, a marker gene of chemotaxis, we constructed a chemotactic SynCom comprising two Pseudomonas strains and one Bacillus strain. In vitro experiments revealed that vitamin C, propionic acid, and esculetin significantly promoted the growth and antagonistic ability of chemotactic SynCom strains. Next, we optimized the proportion of organic fertilizer to support the growth of the SynCom strains. The application of the bioorganic fertilizer containing chemotactic SynCom can protect diseased tobacco field from R. solanacearum invasion and enhance yields. Finally, rhizosphere bacterial community analysis showed that phyla Bacillota and Chloroflexiota were significantly enriched. This study highlights the potential of chemotactic SynCom for effective biocontrol, offering a new approach for managing bacterial wilt and enhancing crop yields. Graphical Abstract Key points • The bioorganic fertilizer containing chemotactic SynCom suppresses R. solanacearum and boosts tobacco yields. • Root exudate-selected prebiotics enhance the antagonistic effects of SynCom against pathogens. • SynCom bioorganic fertilizer regulates the rhizosphere microbiome, enriching beneficial bacterial phyla.https://doi.org/10.1007/s00253-025-13547-6Ralstonia solanacearumChemotaxisBacterial wiltSynComBioorganic fertilizer |
| spellingShingle | Yanxia Liu Weiqi Zhang Li Guo Han Li Jingwei Zhu Xiang Li Xiaoni Zhang Xinrun Yang Yangchun Xu Qirong Shen Tianjie Yang Zhong Wei Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields Applied Microbiology and Biotechnology Ralstonia solanacearum Chemotaxis Bacterial wilt SynCom Bioorganic fertilizer |
| title | Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields |
| title_full | Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields |
| title_fullStr | Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields |
| title_full_unstemmed | Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields |
| title_short | Development of a chemotactic SynCom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields |
| title_sort | development of a chemotactic syncom bioorganic fertilizer for biocontrol of bacterial wilt in tobacco fields |
| topic | Ralstonia solanacearum Chemotaxis Bacterial wilt SynCom Bioorganic fertilizer |
| url | https://doi.org/10.1007/s00253-025-13547-6 |
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