The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice Authors
Introduction: Psoriasis is a chronic inflammatory skin disease depicted by deforming, inconsistent penetrations, and repeated proliferative and inflammatory skin ailment. Local treatment with topical agents is one of the substantial modalities in the remediation of this ailment. Efforts are directed...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Belgorod National Research University
2024-12-01
|
| Series: | Research Results in Pharmacology |
| Subjects: | |
| Online Access: | https://rrpharmacology.ru/index.php/journal/article/view/530 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841554473667788800 |
|---|---|
| author | Zainab A. Mahdi Fouad K. Gatea Omeed M. Hassan Zeena A. Hussein |
| author_facet | Zainab A. Mahdi Fouad K. Gatea Omeed M. Hassan Zeena A. Hussein |
| author_sort | Zainab A. Mahdi |
| collection | DOAJ |
| description | Introduction: Psoriasis is a chronic inflammatory skin disease depicted by deforming, inconsistent penetrations, and repeated proliferative and inflammatory skin ailment. Local treatment with topical agents is one of the substantial modalities in the remediation of this ailment. Efforts are directed toward the development of novel efficacious topical medicaments. The study examined the potential anti-psoriatic effect of topical amlodipine as a 5% ointment in mice based on observational, histopathological, and biomarker outcomes.
Materials and Methods: Forty-five male Swiss Albino mice were randomly allocated into five distinct groups (I-V), with 9 mice in each group (n=9) that underwent shaving of the dorsal hair. In Group I; animals served as control, while animals of other groups received imiquimod (IMQ) on their shaved backs for six consecutive days to induce psoriasis. Groups from III to V received continuous application of IMQ following day 6 along with a vehicle, clobetasol, andamlodipine 5% ointment for 8 consecutive days. During the timeline of the experiment, observation changes were assessed daily, followed by animal euthanasia and sample collection for biochemical and histopathological evaluation.
Results: Topical amlodipine ointment markedly reduced the inflammatory signs of psoriatic lesions, and histopathological inspection confirmed these findings. The levels of interleukin-17 (IL-17), tumor necrosis factor-alpha (TNF-a), and vascular endothelial growth factor (VEGF) were significantly ameliorated by topical amlodipine Ointment in comparison with the untreated imiquimod-induced psoriatic mice group.
Conclusion: The study concludes that topical amlodipine showed an anti-psoriatic effect comparable to that of topical clobetasol owing to antioxidant and anti-inflammatory effects making amlodipine a promising agent in the future of psoriasis management as an adjuvant treatment to the standard. |
| format | Article |
| id | doaj-art-5428241dd91340178cd7675488b448c3 |
| institution | Kabale University |
| issn | 2658-381X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Belgorod National Research University |
| record_format | Article |
| series | Research Results in Pharmacology |
| spelling | doaj-art-5428241dd91340178cd7675488b448c32025-01-08T13:29:06ZengBelgorod National Research UniversityResearch Results in Pharmacology2658-381X2024-12-0110414315310.18413/rrpharmacology.10.530The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice AuthorsZainab A. Mahdi0Fouad K. Gatea1https://orcid.org/0000-0002-4067-7986Omeed M. Hassan2https://orcid.org/0009-0000-3182-5775Zeena A. Hussein3https://orcid.org/0000-0001-7011-5186University of Kirkuk Al-Nahrain UniversityUniversity of KirkukAl-Nahrain UniversityIntroduction: Psoriasis is a chronic inflammatory skin disease depicted by deforming, inconsistent penetrations, and repeated proliferative and inflammatory skin ailment. Local treatment with topical agents is one of the substantial modalities in the remediation of this ailment. Efforts are directed toward the development of novel efficacious topical medicaments. The study examined the potential anti-psoriatic effect of topical amlodipine as a 5% ointment in mice based on observational, histopathological, and biomarker outcomes. Materials and Methods: Forty-five male Swiss Albino mice were randomly allocated into five distinct groups (I-V), with 9 mice in each group (n=9) that underwent shaving of the dorsal hair. In Group I; animals served as control, while animals of other groups received imiquimod (IMQ) on their shaved backs for six consecutive days to induce psoriasis. Groups from III to V received continuous application of IMQ following day 6 along with a vehicle, clobetasol, andamlodipine 5% ointment for 8 consecutive days. During the timeline of the experiment, observation changes were assessed daily, followed by animal euthanasia and sample collection for biochemical and histopathological evaluation. Results: Topical amlodipine ointment markedly reduced the inflammatory signs of psoriatic lesions, and histopathological inspection confirmed these findings. The levels of interleukin-17 (IL-17), tumor necrosis factor-alpha (TNF-a), and vascular endothelial growth factor (VEGF) were significantly ameliorated by topical amlodipine Ointment in comparison with the untreated imiquimod-induced psoriatic mice group. Conclusion: The study concludes that topical amlodipine showed an anti-psoriatic effect comparable to that of topical clobetasol owing to antioxidant and anti-inflammatory effects making amlodipine a promising agent in the future of psoriasis management as an adjuvant treatment to the standard.https://rrpharmacology.ru/index.php/journal/article/view/530amlodipinecalcium channel blockersvascular endothelial growth factorinterleukin-17psoriasisimiquimod |
| spellingShingle | Zainab A. Mahdi Fouad K. Gatea Omeed M. Hassan Zeena A. Hussein The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice Authors Research Results in Pharmacology amlodipine calcium channel blockers vascular endothelial growth factor interleukin-17 psoriasis imiquimod |
| title | The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice Authors |
| title_full | The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice Authors |
| title_fullStr | The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice Authors |
| title_full_unstemmed | The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice Authors |
| title_short | The mitigating effect of a topical preparation of amlodipine on imiquimod-induced psoriasis-like lesion in mice Authors |
| title_sort | mitigating effect of a topical preparation of amlodipine on imiquimod induced psoriasis like lesion in mice authors |
| topic | amlodipine calcium channel blockers vascular endothelial growth factor interleukin-17 psoriasis imiquimod |
| url | https://rrpharmacology.ru/index.php/journal/article/view/530 |
| work_keys_str_mv | AT zainabamahdi themitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors AT fouadkgatea themitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors AT omeedmhassan themitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors AT zeenaahussein themitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors AT zainabamahdi mitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors AT fouadkgatea mitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors AT omeedmhassan mitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors AT zeenaahussein mitigatingeffectofatopicalpreparationofamlodipineonimiquimodinducedpsoriasislikelesioninmiceauthors |