Hepatitis E virus infection in pigs: a first report from Zambia
While evidence suggests presence of HEV infection in humans in Zambia, currently, there is no information on its occurrence in domestic pigs. Here, we investigated the presence of HEV antibodies and genome in domestic pigs in Zambia. Sera (n = 484) from domestic pigs were screened for antibodies aga...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.2002669 |
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| author | Herman M. Chambaro Michihito Sasaki Walter Muleya Masahiro Kajihara Misheck Shawa Kabemba E. Mwape Hayato Harima Yongjin Qiu William W. Hall Paul Fandamu David Squarre Edgar Simulundu Hirofumi Sawa Yasuko Orba |
| author_facet | Herman M. Chambaro Michihito Sasaki Walter Muleya Masahiro Kajihara Misheck Shawa Kabemba E. Mwape Hayato Harima Yongjin Qiu William W. Hall Paul Fandamu David Squarre Edgar Simulundu Hirofumi Sawa Yasuko Orba |
| author_sort | Herman M. Chambaro |
| collection | DOAJ |
| description | While evidence suggests presence of HEV infection in humans in Zambia, currently, there is no information on its occurrence in domestic pigs. Here, we investigated the presence of HEV antibodies and genome in domestic pigs in Zambia. Sera (n = 484) from domestic pigs were screened for antibodies against HEV by ELISA while genome detection in fecal (n = 25) and liver (n = 100) samples from slaughter pigs was conducted using nested RT–PCR assay. Overall, seroprevalence was 47.7% (231/484) while zoonotic genotype 3 HEV RNA was detected in 16.0% (20/125) of slaughtered pigs. This is the first report to highlight occurrence of HEV infection in domestic pigs in Zambia. This finding suggests possible contamination of the pork supply chain. Moreover, there is a potential risk of zoonotic transmission of HEV to abattoir workers, pig farmers and handlers. |
| format | Article |
| id | doaj-art-5403431abe7e41b8b13a153d51bcc076 |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-5403431abe7e41b8b13a153d51bcc0762025-08-20T02:59:13ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-011012169217210.1080/22221751.2021.2002669Hepatitis E virus infection in pigs: a first report from ZambiaHerman M. Chambaro0Michihito Sasaki1Walter Muleya2Masahiro Kajihara3Misheck Shawa4Kabemba E. Mwape5Hayato Harima6Yongjin Qiu7William W. Hall8Paul Fandamu9David Squarre10Edgar Simulundu11Hirofumi Sawa12Yasuko Orba13Division of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanDepartment of Biomedical Sciences, School of Veterinary Medicine, University of Zambia, Lusaka, ZambiaDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanDepartment of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka, ZambiaDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanNational Virus Reference Laboratory, University College Dublin, Dublin, IrelandDepartment of Veterinary Services, Ministry of Fisheries and Livestock, Lusaka, ZambiaDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanDepartment of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, ZambiaDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanDivision of Molecular Pathobiology, International Institute for Zoonosis Control, Sapporo, JapanWhile evidence suggests presence of HEV infection in humans in Zambia, currently, there is no information on its occurrence in domestic pigs. Here, we investigated the presence of HEV antibodies and genome in domestic pigs in Zambia. Sera (n = 484) from domestic pigs were screened for antibodies against HEV by ELISA while genome detection in fecal (n = 25) and liver (n = 100) samples from slaughter pigs was conducted using nested RT–PCR assay. Overall, seroprevalence was 47.7% (231/484) while zoonotic genotype 3 HEV RNA was detected in 16.0% (20/125) of slaughtered pigs. This is the first report to highlight occurrence of HEV infection in domestic pigs in Zambia. This finding suggests possible contamination of the pork supply chain. Moreover, there is a potential risk of zoonotic transmission of HEV to abattoir workers, pig farmers and handlers.https://www.tandfonline.com/doi/10.1080/22221751.2021.2002669Hepatitis E virusgenotype 3seroprevalencedomestic pigZambia |
| spellingShingle | Herman M. Chambaro Michihito Sasaki Walter Muleya Masahiro Kajihara Misheck Shawa Kabemba E. Mwape Hayato Harima Yongjin Qiu William W. Hall Paul Fandamu David Squarre Edgar Simulundu Hirofumi Sawa Yasuko Orba Hepatitis E virus infection in pigs: a first report from Zambia Emerging Microbes and Infections Hepatitis E virus genotype 3 seroprevalence domestic pig Zambia |
| title | Hepatitis E virus infection in pigs: a first report from Zambia |
| title_full | Hepatitis E virus infection in pigs: a first report from Zambia |
| title_fullStr | Hepatitis E virus infection in pigs: a first report from Zambia |
| title_full_unstemmed | Hepatitis E virus infection in pigs: a first report from Zambia |
| title_short | Hepatitis E virus infection in pigs: a first report from Zambia |
| title_sort | hepatitis e virus infection in pigs a first report from zambia |
| topic | Hepatitis E virus genotype 3 seroprevalence domestic pig Zambia |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.2002669 |
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