Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings
The purpose of coatings is to protect or enhance the functionality of the substrate material, irrespective of the field in which the material was designed. The use of coatings in medicine is rapidly expanding with the objective of enhancing the osseointegration ability of metallic materials such as...
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MDPI AG
2024-11-01
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| author | Cosmin M. Cotrut Alexandru Blidisel Diana M. Vranceanu Alina Vladescu (Dragomir) Elena Ungureanu Iulian Pana Mihaela Dinu Catalin Vitelaru Anca C. Parau Vasile Pruna Mihai S. Magurean Irina Titorencu |
| author_facet | Cosmin M. Cotrut Alexandru Blidisel Diana M. Vranceanu Alina Vladescu (Dragomir) Elena Ungureanu Iulian Pana Mihaela Dinu Catalin Vitelaru Anca C. Parau Vasile Pruna Mihai S. Magurean Irina Titorencu |
| author_sort | Cosmin M. Cotrut |
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| description | The purpose of coatings is to protect or enhance the functionality of the substrate material, irrespective of the field in which the material was designed. The use of coatings in medicine is rapidly expanding with the objective of enhancing the osseointegration ability of metallic materials such as titanium. The aim of this study was to obtain biomimetic hydroxyapatite (HAp)-based coatings on titanium by using the pulsed galvanostatic method. The morphology of the HAp-based coatings revealed the presence of very thin and wide plate-like crystals, grown perpendicular to the Ti substrate, while the chemical composition highlighted a Ca/P ratio of 1.66, which is close to that of stoichiometric HAp (1.67). The main phases and chemical bonds identified confirmed the presence of the HAp phase in the developed coatings. A roughness of 228 nm and a contact angle of approx. 17° were obtained for the HAp coatings, highlighting a hydrophilic character. In terms of biomineralization and electrochemical behavior, it was shown that the HAp coatings have significantly enhanced the titanium properties. Finally, the in vitro cell tests carried out with human mesenchymal stem cells showed that the Ti samples coated with HAp have increased cell viability, extracellular matrix, and Ca intracellular deposition when compared with the uncoated Ti, indicating the beneficial effect. |
| format | Article |
| id | doaj-art-53ffeb4369164abaaef76bbde6e32f87 |
| institution | OA Journals |
| issn | 2313-7673 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomimetics |
| spelling | doaj-art-53ffeb4369164abaaef76bbde6e32f872025-08-20T02:28:11ZengMDPI AGBiomimetics2313-76732024-11-0191170410.3390/biomimetics9110704Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite CoatingsCosmin M. Cotrut0Alexandru Blidisel1Diana M. Vranceanu2Alina Vladescu (Dragomir)3Elena Ungureanu4Iulian Pana5Mihaela Dinu6Catalin Vitelaru7Anca C. Parau8Vasile Pruna9Mihai S. Magurean10Irina Titorencu11Faculty of Materials Science and Engineering, National University of Science and Technology Politehnica Bucharest, 313 Independentei Street, 060042 Bucharest, RomaniaHepato-Bilio-Pancreatic Surgery Center, University Clinic Surgical Semiology and Thoracic Surgery, “Victor Babes” University of Medicine and Pharmacy, Sq. Eftimie Murgu No. 2, 300041 Timisoara, RomaniaFaculty of Materials Science and Engineering, National University of Science and Technology Politehnica Bucharest, 313 Independentei Street, 060042 Bucharest, RomaniaDepartment for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, RomaniaFaculty of Materials Science and Engineering, National University of Science and Technology Politehnica Bucharest, 313 Independentei Street, 060042 Bucharest, RomaniaDepartment for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, RomaniaDepartment for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, RomaniaDepartment for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, RomaniaDepartment for Advanced Surface Processing and Analysis by Vacuum Technologies, National Institute of Research and Development for Optoelectronics—INOE 2000, 077125 Magurele, RomaniaRomanian Academy Institute of Cellular Biology and Pathology “Nicolae Simionescu”, 8 B.P. Hasdeu, 050568 Bucharest, RomaniaColentina Clinical Hospital, 020125 Bucharest, RomaniaRomanian Academy Institute of Cellular Biology and Pathology “Nicolae Simionescu”, 8 B.P. Hasdeu, 050568 Bucharest, RomaniaThe purpose of coatings is to protect or enhance the functionality of the substrate material, irrespective of the field in which the material was designed. The use of coatings in medicine is rapidly expanding with the objective of enhancing the osseointegration ability of metallic materials such as titanium. The aim of this study was to obtain biomimetic hydroxyapatite (HAp)-based coatings on titanium by using the pulsed galvanostatic method. The morphology of the HAp-based coatings revealed the presence of very thin and wide plate-like crystals, grown perpendicular to the Ti substrate, while the chemical composition highlighted a Ca/P ratio of 1.66, which is close to that of stoichiometric HAp (1.67). The main phases and chemical bonds identified confirmed the presence of the HAp phase in the developed coatings. A roughness of 228 nm and a contact angle of approx. 17° were obtained for the HAp coatings, highlighting a hydrophilic character. In terms of biomineralization and electrochemical behavior, it was shown that the HAp coatings have significantly enhanced the titanium properties. Finally, the in vitro cell tests carried out with human mesenchymal stem cells showed that the Ti samples coated with HAp have increased cell viability, extracellular matrix, and Ca intracellular deposition when compared with the uncoated Ti, indicating the beneficial effect.https://www.mdpi.com/2313-7673/9/11/704biomimeticsurface modificationhydroxyapatite coatingselectrochemical depositionbiocompatibility |
| spellingShingle | Cosmin M. Cotrut Alexandru Blidisel Diana M. Vranceanu Alina Vladescu (Dragomir) Elena Ungureanu Iulian Pana Mihaela Dinu Catalin Vitelaru Anca C. Parau Vasile Pruna Mihai S. Magurean Irina Titorencu Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings Biomimetics biomimetic surface modification hydroxyapatite coatings electrochemical deposition biocompatibility |
| title | Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings |
| title_full | Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings |
| title_fullStr | Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings |
| title_full_unstemmed | Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings |
| title_short | Evaluation of the In Vitro Behavior of Electrochemically Deposited Plate-like Crystal Hydroxyapatite Coatings |
| title_sort | evaluation of the in vitro behavior of electrochemically deposited plate like crystal hydroxyapatite coatings |
| topic | biomimetic surface modification hydroxyapatite coatings electrochemical deposition biocompatibility |
| url | https://www.mdpi.com/2313-7673/9/11/704 |
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