Genetic diversity and population structure of Aedes caspius in Iran

Abstract The floodwater mosquito Aedes caspius is a competent vector of several human pathogens and has a global distribution across Asia, Europe, Africa, and the Americas. This study aimed to investigate the genetic diversity and population genetic structure of Ae. caspius from Iran. Aedes caspius...

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Main Authors: Azim Paksa, Alireza Sanei‑Dehkordi, Saeed Shahabi, Yaser Salim Abadi, Saideh Yousefi
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-99340-x
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author Azim Paksa
Alireza Sanei‑Dehkordi
Saeed Shahabi
Yaser Salim Abadi
Saideh Yousefi
author_facet Azim Paksa
Alireza Sanei‑Dehkordi
Saeed Shahabi
Yaser Salim Abadi
Saideh Yousefi
author_sort Azim Paksa
collection DOAJ
description Abstract The floodwater mosquito Aedes caspius is a competent vector of several human pathogens and has a global distribution across Asia, Europe, Africa, and the Americas. This study aimed to investigate the genetic diversity and population genetic structure of Ae. caspius from Iran. Aedes caspius mosquitoes were collected from Iran, and genetic diversity was assessed using the mitochondrial DNA-encoded cytochrome c oxidase subunit I (mtDNA-COI) gene. BLAST analysis confirmed the morphological identification of all specimens as Ae. caspius, with 100% similarity to GenBank sequences. Haplotype diversity (Hd) and haplotype diversity variance of the Ae. caspius mosquitoes based on the COI gene were found to be 0.954 and 0.00006, respectively. Nucleotide diversity (Pi) of the Ae. caspius sequences based on the COI gene was calculated to be 0.01495, with 56 segregation sites identified. Phylogenetic analysis demonstrated high genetic diversity in Iranian Ae. caspius populations, suggesting its long-standing establishment in Iran and indicating its native status. The origin of this population in Iran may be linked to populations from the United Arab Emirates (UAE), Pakistan, China, and Europe. Further studies should explore the genetic variability of Ae. caspius in various countries and continents.
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spelling doaj-art-53ef426eba2b49ddb0ce00574757a26c2025-08-20T02:55:38ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-99340-xGenetic diversity and population structure of Aedes caspius in IranAzim Paksa0Alireza Sanei‑Dehkordi1Saeed Shahabi2Yaser Salim Abadi3Saideh Yousefi4Department of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical SciencesInfectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical SciencesDepartment of Biology and Control of Disease Vectors, School of Health, Shiraz University of Medical SciencesDepartment of Health Services and Health Promotion, School of Health, Rafsanjan University of Medical SciencesSirjan School of Medical SciencesAbstract The floodwater mosquito Aedes caspius is a competent vector of several human pathogens and has a global distribution across Asia, Europe, Africa, and the Americas. This study aimed to investigate the genetic diversity and population genetic structure of Ae. caspius from Iran. Aedes caspius mosquitoes were collected from Iran, and genetic diversity was assessed using the mitochondrial DNA-encoded cytochrome c oxidase subunit I (mtDNA-COI) gene. BLAST analysis confirmed the morphological identification of all specimens as Ae. caspius, with 100% similarity to GenBank sequences. Haplotype diversity (Hd) and haplotype diversity variance of the Ae. caspius mosquitoes based on the COI gene were found to be 0.954 and 0.00006, respectively. Nucleotide diversity (Pi) of the Ae. caspius sequences based on the COI gene was calculated to be 0.01495, with 56 segregation sites identified. Phylogenetic analysis demonstrated high genetic diversity in Iranian Ae. caspius populations, suggesting its long-standing establishment in Iran and indicating its native status. The origin of this population in Iran may be linked to populations from the United Arab Emirates (UAE), Pakistan, China, and Europe. Further studies should explore the genetic variability of Ae. caspius in various countries and continents.https://doi.org/10.1038/s41598-025-99340-xAedes caspiusmtDNA-COI geneGenetic diversityHaplotype
spellingShingle Azim Paksa
Alireza Sanei‑Dehkordi
Saeed Shahabi
Yaser Salim Abadi
Saideh Yousefi
Genetic diversity and population structure of Aedes caspius in Iran
Scientific Reports
Aedes caspius
mtDNA-COI gene
Genetic diversity
Haplotype
title Genetic diversity and population structure of Aedes caspius in Iran
title_full Genetic diversity and population structure of Aedes caspius in Iran
title_fullStr Genetic diversity and population structure of Aedes caspius in Iran
title_full_unstemmed Genetic diversity and population structure of Aedes caspius in Iran
title_short Genetic diversity and population structure of Aedes caspius in Iran
title_sort genetic diversity and population structure of aedes caspius in iran
topic Aedes caspius
mtDNA-COI gene
Genetic diversity
Haplotype
url https://doi.org/10.1038/s41598-025-99340-x
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