Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass Spectrometry
A novel and efficient analytical protocol based on paper spray tandem mass spectrometry was developed for the determination of isomeric <i>O</i>-glycoside flavanones in citrus juices and beverages. This approach significantly reduces sample preparation time and solvent consumption compar...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/14/1/20 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589325649313792 |
|---|---|
| author | Lucia Bartella Fabio Mazzotti Ilaria Santoro Ines Rosita Talarico Leonardo Di Donna |
| author_facet | Lucia Bartella Fabio Mazzotti Ilaria Santoro Ines Rosita Talarico Leonardo Di Donna |
| author_sort | Lucia Bartella |
| collection | DOAJ |
| description | A novel and efficient analytical protocol based on paper spray tandem mass spectrometry was developed for the determination of isomeric <i>O</i>-glycoside flavanones in citrus juices and beverages. This approach significantly reduces sample preparation time and solvent consumption compared to traditional chromatographic techniques. By exploiting the unique fragmentation patterns of these compounds, accurate quantification of both diglycosides and their individual isomers (neohesperidoside and rutinose derivatives) was achieved. The method demonstrated excellent analytical performance, with high accuracy, selectivity, and reproducibility. The impact of matrix effects was mitigated through the construction of ratio calibration curves, ensuring reliable quantification in complex matrices. Finally, a simple DPPH experiment to assay the antioxidant activity of each single positional isomer was performed, indicating the superior ability of neohesperidose conjugates. This simplified method offers a valuable tool for quality control, authenticity assessment and the study of health benefits associated with citrus consumption. |
| format | Article |
| id | doaj-art-53ea204d464346e092072dc5b07c2cb0 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-53ea204d464346e092072dc5b07c2cb02025-01-24T13:19:10ZengMDPI AGAntioxidants2076-39212024-12-011412010.3390/antiox14010020Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass SpectrometryLucia Bartella0Fabio Mazzotti1Ilaria Santoro2Ines Rosita Talarico3Leonardo Di Donna4Dipartimento di Chimica e Tecnologie Chimiche, Università Della Calabria, Via Pietro Bucci Cubo 12/D, I-87030 Rende, CS, ItalyDipartimento di Chimica e Tecnologie Chimiche, Università Della Calabria, Via Pietro Bucci Cubo 12/D, I-87030 Rende, CS, ItalyQUASIORA Laboratory, AGRINFRA Research Net, Università Della Calabria, Via Pietro Bucci Cubo 12/D, I-87036 Rende, CS, ItalyDipartimento di Chimica e Tecnologie Chimiche, Università Della Calabria, Via Pietro Bucci Cubo 12/D, I-87030 Rende, CS, ItalyDipartimento di Chimica e Tecnologie Chimiche, Università Della Calabria, Via Pietro Bucci Cubo 12/D, I-87030 Rende, CS, ItalyA novel and efficient analytical protocol based on paper spray tandem mass spectrometry was developed for the determination of isomeric <i>O</i>-glycoside flavanones in citrus juices and beverages. This approach significantly reduces sample preparation time and solvent consumption compared to traditional chromatographic techniques. By exploiting the unique fragmentation patterns of these compounds, accurate quantification of both diglycosides and their individual isomers (neohesperidoside and rutinose derivatives) was achieved. The method demonstrated excellent analytical performance, with high accuracy, selectivity, and reproducibility. The impact of matrix effects was mitigated through the construction of ratio calibration curves, ensuring reliable quantification in complex matrices. Finally, a simple DPPH experiment to assay the antioxidant activity of each single positional isomer was performed, indicating the superior ability of neohesperidose conjugates. This simplified method offers a valuable tool for quality control, authenticity assessment and the study of health benefits associated with citrus consumption.https://www.mdpi.com/2076-3921/14/1/20flavanone isomersantioxidant compoundspaper spray ionizationtandem mass spectrometrycitrus juices |
| spellingShingle | Lucia Bartella Fabio Mazzotti Ilaria Santoro Ines Rosita Talarico Leonardo Di Donna Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass Spectrometry Antioxidants flavanone isomers antioxidant compounds paper spray ionization tandem mass spectrometry citrus juices |
| title | Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass Spectrometry |
| title_full | Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass Spectrometry |
| title_fullStr | Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass Spectrometry |
| title_full_unstemmed | Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass Spectrometry |
| title_short | Direct Determination of Flavanone Isomers in Citrus Juice by Paper Spray Tandem Mass Spectrometry |
| title_sort | direct determination of flavanone isomers in citrus juice by paper spray tandem mass spectrometry |
| topic | flavanone isomers antioxidant compounds paper spray ionization tandem mass spectrometry citrus juices |
| url | https://www.mdpi.com/2076-3921/14/1/20 |
| work_keys_str_mv | AT luciabartella directdeterminationofflavanoneisomersincitrusjuicebypaperspraytandemmassspectrometry AT fabiomazzotti directdeterminationofflavanoneisomersincitrusjuicebypaperspraytandemmassspectrometry AT ilariasantoro directdeterminationofflavanoneisomersincitrusjuicebypaperspraytandemmassspectrometry AT inesrositatalarico directdeterminationofflavanoneisomersincitrusjuicebypaperspraytandemmassspectrometry AT leonardodidonna directdeterminationofflavanoneisomersincitrusjuicebypaperspraytandemmassspectrometry |