Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors
Cholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), play critical roles in neurotransmission and are key targets for inhibitors with therapeutic and toxicological significance. This review focuses on the development and application of fluorometric and colorime...
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MDPI AG
2025-04-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/25/9/2674 |
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| author | Miroslav Pohanka |
| author_facet | Miroslav Pohanka |
| author_sort | Miroslav Pohanka |
| collection | DOAJ |
| description | Cholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), play critical roles in neurotransmission and are key targets for inhibitors with therapeutic and toxicological significance. This review focuses on the development and application of fluorometric and colorimetric biosensors for the detection of cholinesterase inhibitors. These biosensors take advantage of the unique properties of AChE and BChE to provide sensitive and selective detection methods essential for environmental monitoring, food safety, and clinical diagnostics. Recent advances in assay techniques, including the use of gold nanoparticles, pseudoperoxidase nanomaterials, and innovative enzyme–substrate interactions, are highlighted. This review also discusses challenges and future directions for optimizing these biosensors for practical applications, emphasizing their potential to enhance public health and safety. |
| format | Article |
| id | doaj-art-53e7985f48424162ad812bd0bb21bca7 |
| institution | Kabale University |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-53e7985f48424162ad812bd0bb21bca72025-08-20T03:53:01ZengMDPI AGSensors1424-82202025-04-01259267410.3390/s25092674Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase InhibitorsMiroslav Pohanka0Military Faculty of Medicine, University of Defense, Trebesska 1575, 500 02 Hradec Kralove, Czech RepublicCholinesterases, specifically acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), play critical roles in neurotransmission and are key targets for inhibitors with therapeutic and toxicological significance. This review focuses on the development and application of fluorometric and colorimetric biosensors for the detection of cholinesterase inhibitors. These biosensors take advantage of the unique properties of AChE and BChE to provide sensitive and selective detection methods essential for environmental monitoring, food safety, and clinical diagnostics. Recent advances in assay techniques, including the use of gold nanoparticles, pseudoperoxidase nanomaterials, and innovative enzyme–substrate interactions, are highlighted. This review also discusses challenges and future directions for optimizing these biosensors for practical applications, emphasizing their potential to enhance public health and safety.https://www.mdpi.com/1424-8220/25/9/2674acetylcholinesteraseassaybutyrylcholinesteraseenzymegold nanoparticlesnerve agents |
| spellingShingle | Miroslav Pohanka Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors Sensors acetylcholinesterase assay butyrylcholinesterase enzyme gold nanoparticles nerve agents |
| title | Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors |
| title_full | Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors |
| title_fullStr | Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors |
| title_full_unstemmed | Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors |
| title_short | Fluorometric and Colorimetric Biosensors for the Assay of Cholinesterase Inhibitors |
| title_sort | fluorometric and colorimetric biosensors for the assay of cholinesterase inhibitors |
| topic | acetylcholinesterase assay butyrylcholinesterase enzyme gold nanoparticles nerve agents |
| url | https://www.mdpi.com/1424-8220/25/9/2674 |
| work_keys_str_mv | AT miroslavpohanka fluorometricandcolorimetricbiosensorsfortheassayofcholinesteraseinhibitors |