A comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goats
Abstract Renowned for its invaluable undercoat, the cashmere goat is well known. The growth of cashmere fibre initiates when the relatively inactive telogen stage transitions to the anagen stage, which involves active proliferation. However, the molecular mechanisms responsible for this process are...
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BMC
2025-02-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-025-04608-z |
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| author | Rile Nai Chongyan Zhang Yuchun Xie Duhu Man Haijun Li Lina Ma Lu Mi Meng Zhao Qier Mu Lixia Gao Zhihong Liu Jinquan Li |
| author_facet | Rile Nai Chongyan Zhang Yuchun Xie Duhu Man Haijun Li Lina Ma Lu Mi Meng Zhao Qier Mu Lixia Gao Zhihong Liu Jinquan Li |
| author_sort | Rile Nai |
| collection | DOAJ |
| description | Abstract Renowned for its invaluable undercoat, the cashmere goat is well known. The growth of cashmere fibre initiates when the relatively inactive telogen stage transitions to the anagen stage, which involves active proliferation. However, the molecular mechanisms responsible for this process are still unclear. Here, SWATH mass spectrometry (MS), a comparative proteomic analysis, was conducted to examine the proteomic alterations in Inner Mongolia cashmere goat skin samples at two different developmental stages (anagen and telogen). In total, 2414 proteins were detected, with 631 proteins showing differential regulation (503 upregulated proteins and 128 downregulated proteins). Bioinformatic analysis revealed that these proteins, which are differentially regulated, play crucial roles in the pathways associated with metabolism and fatty acids according to the GO and KEGG analyses. Furthermore, interactome analysis revealed that differentially regulated keratins have a crucial impact. The localization of KRT25, KRT71, and KRT82 using immunohistochemistry revealed that these proteins were expressed in the secondary hair follicles of cashmere goat skin. The keratin family plays an irreplaceable and important role in the process of hair follicle growth. |
| format | Article |
| id | doaj-art-53e7555d8fb64b38ab2a5dc5064f3ef3 |
| institution | DOAJ |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-53e7555d8fb64b38ab2a5dc5064f3ef32025-08-20T03:03:58ZengBMCBMC Veterinary Research1746-61482025-02-0121111210.1186/s12917-025-04608-zA comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goatsRile Nai0Chongyan Zhang1Yuchun Xie2Duhu Man3Haijun Li4Lina Ma5Lu Mi6Meng Zhao7Qier Mu8Lixia Gao9Zhihong Liu10Jinquan Li11College of Animal Science, Inner Mongolia Agricultural UniversityCollege of Animal Science, Inner Mongolia Agricultural UniversityCollege of Animal Science and Technology, Hebei Normal University of Science and TechnologyCollege of Agriculture, Hulunbuir UniversityCollege of Veterinary Medicine, Inner Mongolia Agricultural UniversityCollege of Animal Science, Inner Mongolia Agricultural UniversityCollege of Animal Science, Inner Mongolia Agricultural UniversityInner Mongolia Academy of Agriculture and Animal Husbandry SciencesCollege of Animal Science, Inner Mongolia Agricultural UniversityBaotou Light Industry Vocational Technical CollegeCollege of Animal Science, Inner Mongolia Agricultural UniversityCollege of Animal Science, Inner Mongolia Agricultural UniversityAbstract Renowned for its invaluable undercoat, the cashmere goat is well known. The growth of cashmere fibre initiates when the relatively inactive telogen stage transitions to the anagen stage, which involves active proliferation. However, the molecular mechanisms responsible for this process are still unclear. Here, SWATH mass spectrometry (MS), a comparative proteomic analysis, was conducted to examine the proteomic alterations in Inner Mongolia cashmere goat skin samples at two different developmental stages (anagen and telogen). In total, 2414 proteins were detected, with 631 proteins showing differential regulation (503 upregulated proteins and 128 downregulated proteins). Bioinformatic analysis revealed that these proteins, which are differentially regulated, play crucial roles in the pathways associated with metabolism and fatty acids according to the GO and KEGG analyses. Furthermore, interactome analysis revealed that differentially regulated keratins have a crucial impact. The localization of KRT25, KRT71, and KRT82 using immunohistochemistry revealed that these proteins were expressed in the secondary hair follicles of cashmere goat skin. The keratin family plays an irreplaceable and important role in the process of hair follicle growth.https://doi.org/10.1186/s12917-025-04608-zCashmere goatProteomicsHair follicleTelogenAnagen |
| spellingShingle | Rile Nai Chongyan Zhang Yuchun Xie Duhu Man Haijun Li Lina Ma Lu Mi Meng Zhao Qier Mu Lixia Gao Zhihong Liu Jinquan Li A comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goats BMC Veterinary Research Cashmere goat Proteomics Hair follicle Telogen Anagen |
| title | A comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goats |
| title_full | A comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goats |
| title_fullStr | A comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goats |
| title_full_unstemmed | A comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goats |
| title_short | A comparative proteomic-based study identifies essential factors involved in hair follicle growth in inner Mongolia cashmere goats |
| title_sort | comparative proteomic based study identifies essential factors involved in hair follicle growth in inner mongolia cashmere goats |
| topic | Cashmere goat Proteomics Hair follicle Telogen Anagen |
| url | https://doi.org/10.1186/s12917-025-04608-z |
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