Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis Severity

<i>Background and Objectives</i>: The role of adenosine deaminase (ADA) in pulmonary tuberculosis (PTB) remains insufficiently defined in advanced forms of disease. Likewise, the systemic immune inflammatory index (SII) has not been validated in severe PTB. This 6-year prospective observ...

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Main Authors: Ioan Anton Arghir, Oana Cristina Arghir, Marina Ruxandra Otelea, Iulia Tania Andronache, Ileana Ion
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Medicina
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Online Access:https://www.mdpi.com/1648-9144/61/6/1096
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author Ioan Anton Arghir
Oana Cristina Arghir
Marina Ruxandra Otelea
Iulia Tania Andronache
Ileana Ion
author_facet Ioan Anton Arghir
Oana Cristina Arghir
Marina Ruxandra Otelea
Iulia Tania Andronache
Ileana Ion
author_sort Ioan Anton Arghir
collection DOAJ
description <i>Background and Objectives</i>: The role of adenosine deaminase (ADA) in pulmonary tuberculosis (PTB) remains insufficiently defined in advanced forms of disease. Likewise, the systemic immune inflammatory index (SII) has not been validated in severe PTB. This 6-year prospective observational study aims to evaluate biomarker signatures of serum ADA and SII. <i>Materials and Methods</i>: According to the PTB case definition, 232 adult patients were divided into group 1, with a positive bacteriologic exam (n = 168), and group 2, without bacteriological confirmation (n = 64). ADA serum levels were compared by age, gender, nutritional status, morphologic and bacteriological pattern of PTB lesions, survival status, along with serum levels of other inflammatory biomarkers. All patients with comorbidities, interfering with the level of ADA, were excluded to avoid bias. <i>Results</i>: A total cohort of 208 PTB adults, aged 54.37 ± 14.365 years, included 156 males. The overall mortality was 11.53%. Death occurred after a mean interval of 1.63 ± 3.228 months after PTB diagnosis. ADA serum mean levels were 32.94 ± 9.146 IU/L, significantly higher in G1 (<i>p</i> = 0.002), in patients with delayed diagnosis of PTB (<i>p</i> = 0.000), with lung cavitation (<i>p</i> = 0.003), and death as a poor outcome (<i>p</i> ˂ 0.02). SII had a mean value of 1752.226 ± 2704.150, significantly increased in bacteriologically confirmed PTB cases (<i>p</i> = 0.018), delayed diagnosis (<i>p</i> = 0.002), cavitary advanced pulmonary tuberculosis (APT) (<i>p</i> = 0.002), and deceased (<i>p</i> = 0.003). Both an ADA cut-off elevated risk value of over 30 IU/L and SII of over 902 were fulfilled by 73 patients, with 2.10 higher risk of advanced PTB (<i>p</i> = 0.006) and 4.49 higher risk of mortality (<i>p</i> = 0.000). <i>Conclusions</i>: Serum ADA and SII are recommended as predictors of advanced and severe pulmonary TB. These findings indicate that ADA and SII, when elevated together, delineate a high-risk PTB phenotype with greater disease severity and early mortality. The combination offers a pragmatic, biomarker-based approach to risk stratification in PTB.
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spelling doaj-art-53e73c96ad0d427db4e79f3812ea80902025-08-20T03:16:19ZengMDPI AGMedicina1010-660X1648-91442025-06-01616109610.3390/medicina61061096Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis SeverityIoan Anton Arghir0Oana Cristina Arghir1Marina Ruxandra Otelea2Iulia Tania Andronache3Ileana Ion4Doctoral School of Medicine, “Ovidius” University of Constanta, 1 University Alley, 900470 Constanta, RomaniaDoctoral School of Medicine, “Ovidius” University of Constanta, 1 University Alley, 900470 Constanta, RomaniaOccupational Diseases Department, Medicine Faculty, “Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari Blvd., 050474 Bucharest, RomaniaInternal Medicine Clinic, “Dr. Alexandru Gafencu” Military Emergency Hospital, 96 Mamaia Blvd., 900527 Constanta, RomaniaDoctoral School of Medicine, “Ovidius” University of Constanta, 1 University Alley, 900470 Constanta, Romania<i>Background and Objectives</i>: The role of adenosine deaminase (ADA) in pulmonary tuberculosis (PTB) remains insufficiently defined in advanced forms of disease. Likewise, the systemic immune inflammatory index (SII) has not been validated in severe PTB. This 6-year prospective observational study aims to evaluate biomarker signatures of serum ADA and SII. <i>Materials and Methods</i>: According to the PTB case definition, 232 adult patients were divided into group 1, with a positive bacteriologic exam (n = 168), and group 2, without bacteriological confirmation (n = 64). ADA serum levels were compared by age, gender, nutritional status, morphologic and bacteriological pattern of PTB lesions, survival status, along with serum levels of other inflammatory biomarkers. All patients with comorbidities, interfering with the level of ADA, were excluded to avoid bias. <i>Results</i>: A total cohort of 208 PTB adults, aged 54.37 ± 14.365 years, included 156 males. The overall mortality was 11.53%. Death occurred after a mean interval of 1.63 ± 3.228 months after PTB diagnosis. ADA serum mean levels were 32.94 ± 9.146 IU/L, significantly higher in G1 (<i>p</i> = 0.002), in patients with delayed diagnosis of PTB (<i>p</i> = 0.000), with lung cavitation (<i>p</i> = 0.003), and death as a poor outcome (<i>p</i> ˂ 0.02). SII had a mean value of 1752.226 ± 2704.150, significantly increased in bacteriologically confirmed PTB cases (<i>p</i> = 0.018), delayed diagnosis (<i>p</i> = 0.002), cavitary advanced pulmonary tuberculosis (APT) (<i>p</i> = 0.002), and deceased (<i>p</i> = 0.003). Both an ADA cut-off elevated risk value of over 30 IU/L and SII of over 902 were fulfilled by 73 patients, with 2.10 higher risk of advanced PTB (<i>p</i> = 0.006) and 4.49 higher risk of mortality (<i>p</i> = 0.000). <i>Conclusions</i>: Serum ADA and SII are recommended as predictors of advanced and severe pulmonary TB. These findings indicate that ADA and SII, when elevated together, delineate a high-risk PTB phenotype with greater disease severity and early mortality. The combination offers a pragmatic, biomarker-based approach to risk stratification in PTB.https://www.mdpi.com/1648-9144/61/6/1096pulmonary tuberculosisserum adenosine deaminasesystemic immune inflammatory indexbiomarkers
spellingShingle Ioan Anton Arghir
Oana Cristina Arghir
Marina Ruxandra Otelea
Iulia Tania Andronache
Ileana Ion
Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis Severity
Medicina
pulmonary tuberculosis
serum adenosine deaminase
systemic immune inflammatory index
biomarkers
title Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis Severity
title_full Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis Severity
title_fullStr Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis Severity
title_full_unstemmed Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis Severity
title_short Adenosine Deaminase and Systemic Immune Inflammatory Index—A Biomarker Duet Signature of Pulmonary Tuberculosis Severity
title_sort adenosine deaminase and systemic immune inflammatory index a biomarker duet signature of pulmonary tuberculosis severity
topic pulmonary tuberculosis
serum adenosine deaminase
systemic immune inflammatory index
biomarkers
url https://www.mdpi.com/1648-9144/61/6/1096
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