Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages
ObjectivesThis study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation.Materials and MethodsCell viability assays, cell adherence, yeast phagocytosis of Saccharomyc...
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Korean Academy of Conservative Dentistry
2022-08-01
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| Series: | Restorative Dentistry & Endodontics |
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| Online Access: | http://www.rde.ac/upload/pdf/rde-47-e31.pdf |
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| author | Danilo Couto da Silva Leonardo Gomes Vaz Warley Luciano Fonseca Tavares Leda Quercia Vieira Ricardo Reis de Oliveira Antônio Paulino Ribeiro Sobrinho |
| author_facet | Danilo Couto da Silva Leonardo Gomes Vaz Warley Luciano Fonseca Tavares Leda Quercia Vieira Ricardo Reis de Oliveira Antônio Paulino Ribeiro Sobrinho |
| author_sort | Danilo Couto da Silva |
| collection | DOAJ |
| description | ObjectivesThis study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation.Materials and MethodsCell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not.ResultsAfter long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS.ConclusionsOur results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters. |
| format | Article |
| id | doaj-art-53d786a6748e4b3cae8b7cc93db25291 |
| institution | OA Journals |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2022-08-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-53d786a6748e4b3cae8b7cc93db252912025-08-20T02:38:46ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662022-08-01473e3110.5395/rde.2022.47.e311028Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophagesDanilo Couto da Silva0Leonardo Gomes Vaz1Warley Luciano Fonseca Tavares2Leda Quercia Vieira3Ricardo Reis de Oliveira4Antônio Paulino Ribeiro Sobrinho5Department of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, BrazilDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, BrazilDepartment of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, BrazilDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, BrazilDepartment of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, BrazilDepartment of Restorative Dentistry, Faculty of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, BrazilObjectivesThis study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation.Materials and MethodsCell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not.ResultsAfter long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS.ConclusionsOur results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.http://www.rde.ac/upload/pdf/rde-47-e31.pdfcytotoxicitymacrophagesdental cementresin cement |
| spellingShingle | Danilo Couto da Silva Leonardo Gomes Vaz Warley Luciano Fonseca Tavares Leda Quercia Vieira Ricardo Reis de Oliveira Antônio Paulino Ribeiro Sobrinho Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages Restorative Dentistry & Endodontics cytotoxicity macrophages dental cement resin cement |
| title | Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages |
| title_full | Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages |
| title_fullStr | Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages |
| title_full_unstemmed | Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages |
| title_short | Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages |
| title_sort | cytotoxicity of two self adhesive resin cements and their interference in the phagocytic activity of murine macrophages |
| topic | cytotoxicity macrophages dental cement resin cement |
| url | http://www.rde.ac/upload/pdf/rde-47-e31.pdf |
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