Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights
This study focuses on a multifunctional fluorescence probe JFT based on the FRET (Fluorescence Resonance Energy Transfer) and TICT (Twisted Intramolecular Charge Transfer) mechanism. JFT combines an electron donor and an acceptor, enabling it to detect sulfite and monitor intracellular viscosity. Wh...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2025.1642191/full |
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| author | Hua Han Bin Han Yongjin Peng Yongjin Peng Yuling Liu Yuling Liu |
| author_facet | Hua Han Bin Han Yongjin Peng Yongjin Peng Yuling Liu Yuling Liu |
| author_sort | Hua Han |
| collection | DOAJ |
| description | This study focuses on a multifunctional fluorescence probe JFT based on the FRET (Fluorescence Resonance Energy Transfer) and TICT (Twisted Intramolecular Charge Transfer) mechanism. JFT combines an electron donor and an acceptor, enabling it to detect sulfite and monitor intracellular viscosity. When reacting with sulfite, its electronic structure changes, turning off FRET and altering fluorescence wavelength and intensity. In different viscosity environments, the rotation of carbon-carbon bonds in the electron acceptor structure of JFT varies, affecting non-radiative transition pathways and fluorescence intensity. Theoretical calculations based on TDDFT reveal the electron distribution changes before and after the reaction with sulfite species, consistent with experimental phenomena. These findings deepen the understanding of the FRET mechanism of fluorescence probes and offer theoretical guidance for the design of more efficient fluorescence probes. |
| format | Article |
| id | doaj-art-53d39b49b2a44e4591cda176bee0c41d |
| institution | Kabale University |
| issn | 2296-2646 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj-art-53d39b49b2a44e4591cda176bee0c41d2025-08-20T03:51:53ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462025-07-011310.3389/fchem.2025.16421911642191Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insightsHua Han0Bin Han1Yongjin Peng2Yongjin Peng3Yuling Liu4Yuling Liu53rd Affiliated Hospital of Jinzhou Medical University, Jinzhou, ChinaDepartment of Physics, Tianjin Renai College, Tianjin, ChinaCollege of Modern Industry of Health Management, Jinzhou Medical University, Jinzhou, ChinaLiaoning Province Key Laboratory of Human Phenome Research, Jinzhou Medical University, Jinzhou, ChinaCollege of Modern Industry of Health Management, Jinzhou Medical University, Jinzhou, ChinaLiaoning Province Key Laboratory of Human Phenome Research, Jinzhou Medical University, Jinzhou, ChinaThis study focuses on a multifunctional fluorescence probe JFT based on the FRET (Fluorescence Resonance Energy Transfer) and TICT (Twisted Intramolecular Charge Transfer) mechanism. JFT combines an electron donor and an acceptor, enabling it to detect sulfite and monitor intracellular viscosity. When reacting with sulfite, its electronic structure changes, turning off FRET and altering fluorescence wavelength and intensity. In different viscosity environments, the rotation of carbon-carbon bonds in the electron acceptor structure of JFT varies, affecting non-radiative transition pathways and fluorescence intensity. Theoretical calculations based on TDDFT reveal the electron distribution changes before and after the reaction with sulfite species, consistent with experimental phenomena. These findings deepen the understanding of the FRET mechanism of fluorescence probes and offer theoretical guidance for the design of more efficient fluorescence probes.https://www.frontiersin.org/articles/10.3389/fchem.2025.1642191/fullmultifunctionalfluorescent probesulfiteintracellular viscositytheoretical investigation |
| spellingShingle | Hua Han Bin Han Yongjin Peng Yongjin Peng Yuling Liu Yuling Liu Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights Frontiers in Chemistry multifunctional fluorescent probe sulfite intracellular viscosity theoretical investigation |
| title | Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights |
| title_full | Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights |
| title_fullStr | Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights |
| title_full_unstemmed | Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights |
| title_short | Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights |
| title_sort | decoding jft a multifunctional fluorescence probe for sulfite and viscosity insights |
| topic | multifunctional fluorescent probe sulfite intracellular viscosity theoretical investigation |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2025.1642191/full |
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