Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar
Poplar is a tree species used in urban greening, and colored leaf poplar is in great demand because of its higher ornamental value and function. In this study, we generated a golden leaf 84 K poplar (Populus alba × Populus glandulosa) by overexpressing the AtSep2 (stress-enhanced protein) gene from...
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Maximum Academic Press
2024-01-01
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| Series: | Ornamental Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/opr-0024-0016 |
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| author | Donghui Chao Xiaoou Zhai Lulu Ren Shan Fu Jin Zhang Shuang Feng Haizhen Zhang Aimin Zhou |
| author_facet | Donghui Chao Xiaoou Zhai Lulu Ren Shan Fu Jin Zhang Shuang Feng Haizhen Zhang Aimin Zhou |
| author_sort | Donghui Chao |
| collection | DOAJ |
| description | Poplar is a tree species used in urban greening, and colored leaf poplar is in great demand because of its higher ornamental value and function. In this study, we generated a golden leaf 84 K poplar (Populus alba × Populus glandulosa) by overexpressing the AtSep2 (stress-enhanced protein) gene from Arabidopsis. AtSep2 belongs to the light-harvesting chlorophyll (Chl)-binding complex (LHC)-like family and contains a conserved Chl-binding motif. Subcellular localization analysis showed that AtSep2 was mainly localized in the chloroplasts of poplar leaves. AtSep2 overexpression caused the golden leaf phenotype in poplar by reducing total Chl content. Before and after high light stress, the relative reduction in total Chl content and maximum quantum yield of PSII photochemistry (Fv/Fm) in transgenic poplar leaves was lower than that in wild-type (WT) leaves. Transgenic poplar leaves accumulated less reactive oxygen species (ROS) and had a higher net photosynthetic rate (Pn) than WT leaves under high light stress. Overall, AtSep2 overexpression caused the golden leaf phenotype and enhanced the tolerance of poplar leaves to high light stress. The present study suggests that AtSep2 is ideal for the genetic improvement of the golden leaf phenotype in poplar and other ornamental plants. |
| format | Article |
| id | doaj-art-53ca9ba3a73e4f9daf97529c53c22b29 |
| institution | DOAJ |
| issn | 2769-2094 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Ornamental Plant Research |
| spelling | doaj-art-53ca9ba3a73e4f9daf97529c53c22b292025-08-20T03:18:34ZengMaximum Academic PressOrnamental Plant Research2769-20942024-01-01411710.48130/opr-0024-0016opr-0024-0016Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplarDonghui Chao0Xiaoou Zhai1Lulu Ren2Shan Fu3Jin Zhang4Shuang Feng5Haizhen Zhang6Aimin Zhou7College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Forest Botanical Garden, Harbin 150046, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaLarge-Scale Instrument and Equipment Sharing Service Platform, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaPoplar is a tree species used in urban greening, and colored leaf poplar is in great demand because of its higher ornamental value and function. In this study, we generated a golden leaf 84 K poplar (Populus alba × Populus glandulosa) by overexpressing the AtSep2 (stress-enhanced protein) gene from Arabidopsis. AtSep2 belongs to the light-harvesting chlorophyll (Chl)-binding complex (LHC)-like family and contains a conserved Chl-binding motif. Subcellular localization analysis showed that AtSep2 was mainly localized in the chloroplasts of poplar leaves. AtSep2 overexpression caused the golden leaf phenotype in poplar by reducing total Chl content. Before and after high light stress, the relative reduction in total Chl content and maximum quantum yield of PSII photochemistry (Fv/Fm) in transgenic poplar leaves was lower than that in wild-type (WT) leaves. Transgenic poplar leaves accumulated less reactive oxygen species (ROS) and had a higher net photosynthetic rate (Pn) than WT leaves under high light stress. Overall, AtSep2 overexpression caused the golden leaf phenotype and enhanced the tolerance of poplar leaves to high light stress. The present study suggests that AtSep2 is ideal for the genetic improvement of the golden leaf phenotype in poplar and other ornamental plants.https://www.maxapress.com/article/doi/10.48130/opr-0024-0016stress-enhanced proteinpoplargolden leafhigh light tolerance |
| spellingShingle | Donghui Chao Xiaoou Zhai Lulu Ren Shan Fu Jin Zhang Shuang Feng Haizhen Zhang Aimin Zhou Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar Ornamental Plant Research stress-enhanced protein poplar golden leaf high light tolerance |
| title | Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar |
| title_full | Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar |
| title_fullStr | Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar |
| title_full_unstemmed | Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar |
| title_short | Chlorophyll-binding protein AtSep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar |
| title_sort | chlorophyll binding protein atsep2 overexpression causes leaf yellowing and enhances tolerance to high light stress in poplar |
| topic | stress-enhanced protein poplar golden leaf high light tolerance |
| url | https://www.maxapress.com/article/doi/10.48130/opr-0024-0016 |
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