Comparative study of the antioxidant effect of three Aloe species

Research into antioxidants in plants has expanded greatly in recent years, to find the best posible antioxidants to prevent and even treat disease. This study represents the first comparative investigation of the antioxidant activity of these three Aloe species (Aloe vera, arborescens and brevifolia...

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Main Authors: Derouiche Mourad, Chkird Fadoua, Ziani Anas, Boukhaffa Chaymae, Lafdil Mohamed, Alla Anass, Arabi Mourad, Abdelbasset Berrichi, Noureddine Kouddane
Format: Article
Language:English
Published: EDP Sciences 2025-01-01
Series:E3S Web of Conferences
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Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/32/e3sconf_joe52025_01016.pdf
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author Derouiche Mourad
Chkird Fadoua
Ziani Anas
Boukhaffa Chaymae
Lafdil Mohamed
Alla Anass
Arabi Mourad
Abdelbasset Berrichi
Noureddine Kouddane
author_facet Derouiche Mourad
Chkird Fadoua
Ziani Anas
Boukhaffa Chaymae
Lafdil Mohamed
Alla Anass
Arabi Mourad
Abdelbasset Berrichi
Noureddine Kouddane
author_sort Derouiche Mourad
collection DOAJ
description Research into antioxidants in plants has expanded greatly in recent years, to find the best posible antioxidants to prevent and even treat disease. This study represents the first comparative investigation of the antioxidant activity of these three Aloe species (Aloe vera, arborescens and brevifolia). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was used to assess the antioxidant activity of methanolic extracts of the three Aloe species. A calibration range of a standard antioxidant represents the positive control, Butylhydroxyanisol (BHA) (0-100 µg/ml). The results showed a significant difference in the free radical scavenging activity of the three Aloe species and the standard (BHA) for DPPH, with respective IC50 values from lowest to highest of 0.040 mg/ml for standard BHA, 0.100 mg/ml for Aloe vera, 0.270 mg/ml for Aloe arborescens and 0.370 mg/ml for Aloe brevifolia. The percentage of inhibition in Aloe vera is higher than in the other two species (Aloe arborescens and brevifolia). Aloe vera, which is very rich in active substances, showed high antioxidant potential comparable to that of the standard. Leaf extracts from Aloe arborescens and Aloe brevifolia also had a lower antioxidant potential than Aloe vera, but this was not negligible.
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spelling doaj-art-53c9d145a89b42e6b02de0d494a034132025-08-20T02:33:43ZengEDP SciencesE3S Web of Conferences2267-12422025-01-016320101610.1051/e3sconf/202563201016e3sconf_joe52025_01016Comparative study of the antioxidant effect of three Aloe speciesDerouiche Mourad0Chkird Fadoua1Ziani Anas2Boukhaffa Chaymae3Lafdil Mohamed4Alla Anass5Arabi Mourad6Abdelbasset Berrichi7Noureddine Kouddane8Laboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityLaboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityLaboratory of Bioresource, Biotechnology, Ethnopharmacology and Health, Faculty of Science Oujda, Mohammed First UniversityLaboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityLaboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityLaboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityLaboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityLaboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityLaboratory for improving agricultural production, biotechnology, and the environment (LAPABE), Faculty of Science Oujda, Mohammed First UniversityResearch into antioxidants in plants has expanded greatly in recent years, to find the best posible antioxidants to prevent and even treat disease. This study represents the first comparative investigation of the antioxidant activity of these three Aloe species (Aloe vera, arborescens and brevifolia). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay was used to assess the antioxidant activity of methanolic extracts of the three Aloe species. A calibration range of a standard antioxidant represents the positive control, Butylhydroxyanisol (BHA) (0-100 µg/ml). The results showed a significant difference in the free radical scavenging activity of the three Aloe species and the standard (BHA) for DPPH, with respective IC50 values from lowest to highest of 0.040 mg/ml for standard BHA, 0.100 mg/ml for Aloe vera, 0.270 mg/ml for Aloe arborescens and 0.370 mg/ml for Aloe brevifolia. The percentage of inhibition in Aloe vera is higher than in the other two species (Aloe arborescens and brevifolia). Aloe vera, which is very rich in active substances, showed high antioxidant potential comparable to that of the standard. Leaf extracts from Aloe arborescens and Aloe brevifolia also had a lower antioxidant potential than Aloe vera, but this was not negligible.https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/32/e3sconf_joe52025_01016.pdfantioxidantaloe arborescensaloe brevifoliaaloe vera.
spellingShingle Derouiche Mourad
Chkird Fadoua
Ziani Anas
Boukhaffa Chaymae
Lafdil Mohamed
Alla Anass
Arabi Mourad
Abdelbasset Berrichi
Noureddine Kouddane
Comparative study of the antioxidant effect of three Aloe species
E3S Web of Conferences
antioxidant
aloe arborescens
aloe brevifolia
aloe vera.
title Comparative study of the antioxidant effect of three Aloe species
title_full Comparative study of the antioxidant effect of three Aloe species
title_fullStr Comparative study of the antioxidant effect of three Aloe species
title_full_unstemmed Comparative study of the antioxidant effect of three Aloe species
title_short Comparative study of the antioxidant effect of three Aloe species
title_sort comparative study of the antioxidant effect of three aloe species
topic antioxidant
aloe arborescens
aloe brevifolia
aloe vera.
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/32/e3sconf_joe52025_01016.pdf
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