Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology

This study aimed to determine the agreement between margin status in feline mammary tumors’ surgery by comparing 3 margin assessment techniques and to determine the predictive value of prognostic clinical and histological parameters for margin status using intraoperative margin assessment techniques...

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Main Authors: João Martins, Rute Penim, José Dos-Santos, Joana Fonseca, Pedro Faísca, Liege Martins, Nuno Leal, José Catarino, Patrícia C. Cabral, Sónia Campos
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Veterinary Medicine International
Online Access:http://dx.doi.org/10.1155/vmi/5596985
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author João Martins
Rute Penim
José Dos-Santos
Joana Fonseca
Pedro Faísca
Liege Martins
Nuno Leal
José Catarino
Patrícia C. Cabral
Sónia Campos
author_facet João Martins
Rute Penim
José Dos-Santos
Joana Fonseca
Pedro Faísca
Liege Martins
Nuno Leal
José Catarino
Patrícia C. Cabral
Sónia Campos
author_sort João Martins
collection DOAJ
description This study aimed to determine the agreement between margin status in feline mammary tumors’ surgery by comparing 3 margin assessment techniques and to determine the predictive value of prognostic clinical and histological parameters for margin status using intraoperative margin assessment techniques. During the intraoperative period, 69 surgical margins from 12 female cats undergoing unilateral radical mastectomy were assessed by imprint cytology (IC) and scrape cytology (SC), both intraoperative cytological techniques. The excised mastectomy specimens were then evaluated postoperatively by histopathology (HP). Cochran’s Q test was used to determine differences in positive margin detection for the three methods. The agreement between the three methods was assessed using the Fleiss kappa coefficient. A binary logistic regression model was used to assess whether clinical and histological prognostic parameters could predict intraoperative margin status. There was no significant difference in positive margins between the three methods (p=0.174). The agreement between the three methods was poor (Fleiss kappa = −0.020; 95% CI −0.156 to 0.117). Lymphovascular invasion is a significant predictor of a positive intraoperative margin, with an OD of 18.652 (95% CI = 1.742–199.652). These findings suggest that IC and SC are viable methods for assessing the status of surgical margins during surgery for feline mammary tumors. Intraoperative cytology may be complementary to HP, particularly in cases of lymphovascular invasion.
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spelling doaj-art-53c2b70df8b24ebfad4bab72a56a72f02025-08-20T02:13:48ZengWileyVeterinary Medicine International2042-00482025-01-01202510.1155/vmi/5596985Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and HistopathologyJoão Martins0Rute Penim1José Dos-Santos2Joana Fonseca3Pedro Faísca4Liege Martins5Nuno Leal6José Catarino7Patrícia C. Cabral8Sónia Campos9Faculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineFaculty of Veterinary MedicineThis study aimed to determine the agreement between margin status in feline mammary tumors’ surgery by comparing 3 margin assessment techniques and to determine the predictive value of prognostic clinical and histological parameters for margin status using intraoperative margin assessment techniques. During the intraoperative period, 69 surgical margins from 12 female cats undergoing unilateral radical mastectomy were assessed by imprint cytology (IC) and scrape cytology (SC), both intraoperative cytological techniques. The excised mastectomy specimens were then evaluated postoperatively by histopathology (HP). Cochran’s Q test was used to determine differences in positive margin detection for the three methods. The agreement between the three methods was assessed using the Fleiss kappa coefficient. A binary logistic regression model was used to assess whether clinical and histological prognostic parameters could predict intraoperative margin status. There was no significant difference in positive margins between the three methods (p=0.174). The agreement between the three methods was poor (Fleiss kappa = −0.020; 95% CI −0.156 to 0.117). Lymphovascular invasion is a significant predictor of a positive intraoperative margin, with an OD of 18.652 (95% CI = 1.742–199.652). These findings suggest that IC and SC are viable methods for assessing the status of surgical margins during surgery for feline mammary tumors. Intraoperative cytology may be complementary to HP, particularly in cases of lymphovascular invasion.http://dx.doi.org/10.1155/vmi/5596985
spellingShingle João Martins
Rute Penim
José Dos-Santos
Joana Fonseca
Pedro Faísca
Liege Martins
Nuno Leal
José Catarino
Patrícia C. Cabral
Sónia Campos
Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology
Veterinary Medicine International
title Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology
title_full Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology
title_fullStr Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology
title_full_unstemmed Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology
title_short Assessment of Margin Status in Feline Mastectomy: Predictive Factors and Agreement Between Intraoperative Cytology and Histopathology
title_sort assessment of margin status in feline mastectomy predictive factors and agreement between intraoperative cytology and histopathology
url http://dx.doi.org/10.1155/vmi/5596985
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