Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin
Isolation and transplantation of rodent islets are frequently used as a tool for predicting the behavior of new protocols for islet allotransplants in type 1 diabetes patients. Bovine serum albumin (BSA) is recognized as a protease inhibitor possibly protecting function and viability in islets. For...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2012/856386 |
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| author | Suzanne Bertera A. N. Balamurugan Rita Bottino Jing He Massimo Trucco |
| author_facet | Suzanne Bertera A. N. Balamurugan Rita Bottino Jing He Massimo Trucco |
| author_sort | Suzanne Bertera |
| collection | DOAJ |
| description | Isolation and transplantation of rodent islets are frequently used as a tool for predicting the behavior of new protocols for islet allotransplants in type 1 diabetes patients. Bovine serum albumin (BSA) is recognized as a protease inhibitor possibly protecting function and viability in islets. For this study, the addition of 0.2% BSA to the isolation protocol resulted in a 30% increase in islet yields while other parameters, such as viability and function, retained high islet quality. In vivo, a minimal mass of 70 BSA treated islets showed their ability to control glycemia levels in diabetic mice by bringing the average blood glucose to 153±13.2 mg/dL compared to 288±22.6 mg/dL without BSA. Our results show that the simple addition of BSA to the isolation protocol constitutes a reliable and reproducible method for increasing islet yield. Also adding BSA to the transplantation medium improves islet function in vivo. The method outlined here can reduce the overall number of animals needed per experiment and also reduce the time and resources needed for islet preparation. |
| format | Article |
| id | doaj-art-53b0a38166f940d3a4ba537539873251 |
| institution | Kabale University |
| issn | 2090-0007 2090-0015 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Transplantation |
| spelling | doaj-art-53b0a38166f940d3a4ba5375398732512025-02-03T01:11:28ZengWileyJournal of Transplantation2090-00072090-00152012-01-01201210.1155/2012/856386856386Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum AlbuminSuzanne Bertera0A. N. Balamurugan1Rita Bottino2Jing He3Massimo Trucco4Division of Immunogenetics, Department of Pediatrics, John G. Rangos Research Center, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USADepartment of Surgery, University of Minnesota, Minneapolis, MN 55455, USADivision of Immunogenetics, Department of Pediatrics, John G. Rangos Research Center, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USADivision of Immunogenetics, Department of Pediatrics, John G. Rangos Research Center, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USADivision of Immunogenetics, Department of Pediatrics, John G. Rangos Research Center, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201, USAIsolation and transplantation of rodent islets are frequently used as a tool for predicting the behavior of new protocols for islet allotransplants in type 1 diabetes patients. Bovine serum albumin (BSA) is recognized as a protease inhibitor possibly protecting function and viability in islets. For this study, the addition of 0.2% BSA to the isolation protocol resulted in a 30% increase in islet yields while other parameters, such as viability and function, retained high islet quality. In vivo, a minimal mass of 70 BSA treated islets showed their ability to control glycemia levels in diabetic mice by bringing the average blood glucose to 153±13.2 mg/dL compared to 288±22.6 mg/dL without BSA. Our results show that the simple addition of BSA to the isolation protocol constitutes a reliable and reproducible method for increasing islet yield. Also adding BSA to the transplantation medium improves islet function in vivo. The method outlined here can reduce the overall number of animals needed per experiment and also reduce the time and resources needed for islet preparation.http://dx.doi.org/10.1155/2012/856386 |
| spellingShingle | Suzanne Bertera A. N. Balamurugan Rita Bottino Jing He Massimo Trucco Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin Journal of Transplantation |
| title | Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin |
| title_full | Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin |
| title_fullStr | Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin |
| title_full_unstemmed | Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin |
| title_short | Increased Yield and Improved Transplantation Outcome of Mouse Islets with Bovine Serum Albumin |
| title_sort | increased yield and improved transplantation outcome of mouse islets with bovine serum albumin |
| url | http://dx.doi.org/10.1155/2012/856386 |
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